دورية أكاديمية
Biochemical characterization of mitochondria from adult worms and plerocercoid larvae of Spirometra mansoni shows mixed populations of anaerobic and aerobic mitochondria.
| العنوان: | Biochemical characterization of mitochondria from adult worms and plerocercoid larvae of Spirometra mansoni shows mixed populations of anaerobic and aerobic mitochondria. |
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| المؤلفون: | Takamiya S; Department of Tropical Medicine and Parasitology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan. Electronic address: stakamiy@juntendo.ac.jp., Nakamura T; Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara 252-0374, Japan., Tsubokawa D; Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara 252-0374, Japan., Tsuji N; Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Minami-ku, Sagamihara 252-0374, Japan., Fukuda K; Center for Laboratory Animal Science, National Defense Medical College, 3-2 Namiki, Tokorozawa 359-8513, Japan., Yamasaki H; Department of Tropical Medicine and Parasitology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan; Department of Parasitology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan., Fujimura T; Laboratory of Proteomics and Biomolecular Science, Biomedical Research Core Facilities, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan., Taka H; Laboratory of Proteomics and Biomolecular Science, Biomedical Research Core Facilities, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan., Kaga N; Laboratory of Proteomics and Biomolecular Science, Biomedical Research Core Facilities, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan., Kobayashi-Tanabe M; Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, 1-17-71 Fuchinobe Chuou-ku, Sagamihara 252-5201, Japan., Matsuda M; Laboratory of Molecular Biology, Graduate School of Environmental Health Sciences, Azabu University, 1-17-71 Fuchinobe Chuou-ku, Sagamihara 252-5201, Japan., Mita T; Department of Tropical Medicine and Parasitology, Faculty of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan. |
| المصدر: | Parasitology international [Parasitol Int] 2023 Feb; Vol. 92, pp. 102695. Date of Electronic Publication: 2022 Oct 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9708549 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-0329 (Electronic) Linking ISSN: 13835769 NLM ISO Abbreviation: Parasitol Int Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, c1997- |
| مواضيع طبية MeSH: | Sparganum* , Spirometra*, Animals ; Hydrogen Peroxide ; Anaerobiosis ; NAD ; Mitochondria ; Succinates |
| مستخلص: | The mitochondria of adult and plerocercoid Spirometra mansoni were characterized in isolated mitochondria and in situ by electron microscopic histochemistry with special attention to the respiratory chain. Although the specific activities of the constituent enzyme complexes of succinate oxidase are fairly similar in adult and plerocercoid mitochondria, those of succinate oxidase and NADH-FRD are approximately 4- and 25-fold higher in adult mitochondria than in plerocercoid mitochondria, respectively. Quinone analysis by high performance liquid chromatography and mass spectrometry showed that adult and plerocercoid mitochondria contained both rhodoquinone-10 and ubiquinone-10 at concentrations of 4.98 and 0.106 nmol mg -1 for adult, and 0.677 and 0.137 nmol mg -1 for plerocercoid, respectively. Inhibition studies on the succinate-oxidase system of adult mitochondria showed that they possessed both cyanide-sensitive and -insensitive succinate oxidases, the latter of which produces hydrogen peroxide. Adult mitochondria, when NADH was used as a substrate, were shown to produce hydrogen peroxide, and the production of hydrogen peroxide decreased to undetectable levels in the presence of fumarate. The specific activities of NADH-fumarate reductase and cytochrome c oxidase were significantly higher in mature proglottids than in immature and gravid proglottids. Isopycnic density-gradient centrifugation analyses and in situ electron microscopic histochemistry revealed that both adult and plerocercoid mitochondria were heterogeneous in terms of respiratory function and physicochemical properties. The physiological significance of adult and plerocercoid mitochondria is discussed in relation to the oxygen tension of their parasitic habitats. Competing Interests: Declaration of Competing Interest The authors declare that no conflict of interest exists. (Copyright © 2022. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: Adult; Aerobic mitochondria; Anaerobic mitochondria; NADH-fumarate reductase; Plerocercoid; Respiratory chain; Spirometra mansoni |
| المشرفين على المادة: | BBX060AN9V (Hydrogen Peroxide) 0U46U6E8UK (NAD) 0 (Succinates) |
| تواريخ الأحداث: | Date Created: 20221109 Date Completed: 20221122 Latest Revision: 20221122 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.parint.2022.102695 |
| PMID: | 36349608 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1873-0329 |
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| DOI: | 10.1016/j.parint.2022.102695 |