دورية أكاديمية
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Comparison of Optical and Electrical Sensor Characteristics for Efficient Analysis of Attachment and Detachment of Aptamer.

التفاصيل البيبلوغرافية
العنوان: Comparison of Optical and Electrical Sensor Characteristics for Efficient Analysis of Attachment and Detachment of Aptamer.
المؤلفون: Park Y; Department of Biomedical Engineering, College of Life Science and Biotechnology, Dongguk University, Seoul 04620, Korea., Dang TV; Department of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi, Korea., Jeong U; SKhynix, Gyeongchung-daero 2091, Bubal-eup, Incheon-si 17336, Gyeonggi-do, Korea., Kim MI; Department of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi, Korea., Kim J; Department of Biomedical Engineering, College of Life Science and Biotechnology, Dongguk University, Seoul 04620, Korea.
المصدر: Biosensors [Biosensors (Basel)] 2022 Nov 07; Vol. 12 (11). Date of Electronic Publication: 2022 Nov 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI Pub.
مواضيع طبية MeSH: Aptamers, Nucleotide* , Biosensing Techniques* , Graphite* , Nucleic Acids*, Thrombin/analysis ; Limit of Detection
مستخلص: Nucleic acid aptamer-based research has focused on achieving the highest performance for bioassays. However, there are limitations in evaluating the affinity for the target analytes in these nucleic acid aptamer-based bioassays. In this study, we mainly propose graphene oxide (GO)-based electrical and optical analyses to efficiently evaluate the affinity between an aptamer and its target. We found that an aptamer-coupled GO-based chip with an electrical resistance induced by a field-effect transistor, with aptamers as low as 100 pM, can detect the target, thrombin, at yields as low as 250 pM within five minutes. In the optical approach, the fluorescent dye-linked aptamer, as low as 100 nM, was efficiently used with GO, enabling the sensitive detection of thrombin at yields as low as 5 nM. The cantilever type of mechanical analysis also demonstrated the intuitive aptamer-thrombin reaction in the signal using dBm units. Finally, a comparison of electrical and optical sensors' characteristics was introduced in the attachment and detachment of aptamer to propose an efficient analysis that can be utilized for various aptamer-based research fields.
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معلومات مُعتمدة: 20015793 Ministry of Trade, Industry and Energy; NRF-2019R1A2C1087459 Ministry of Science ICT and Future Planning
فهرسة مساهمة: Keywords: aptamer; cantilever-integrated waveguide; field-effect transistor; fluorescence quenching; graphene oxide; thrombin detection
المشرفين على المادة: 0 (Aptamers, Nucleotide)
EC 3.4.21.5 (Thrombin)
0 (graphene oxide)
7782-42-5 (Graphite)
0 (Nucleic Acids)
تواريخ الأحداث: Date Created: 20221110 Date Completed: 20221114 Latest Revision: 20221126
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9688426
DOI: 10.3390/bios12110979
PMID: 36354488
قاعدة البيانات: MEDLINE
الوصف
تدمد:2079-6374
DOI:10.3390/bios12110979