دورية أكاديمية
Comparison of Optical and Electrical Sensor Characteristics for Efficient Analysis of Attachment and Detachment of Aptamer.
العنوان: | Comparison of Optical and Electrical Sensor Characteristics for Efficient Analysis of Attachment and Detachment of Aptamer. |
---|---|
المؤلفون: | Park Y; Department of Biomedical Engineering, College of Life Science and Biotechnology, Dongguk University, Seoul 04620, Korea., Dang TV; Department of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi, Korea., Jeong U; SKhynix, Gyeongchung-daero 2091, Bubal-eup, Incheon-si 17336, Gyeonggi-do, Korea., Kim MI; Department of BioNano Technology, Gachon University, Seongnam 13120, Gyeonggi, Korea., Kim J; Department of Biomedical Engineering, College of Life Science and Biotechnology, Dongguk University, Seoul 04620, Korea. |
المصدر: | Biosensors [Biosensors (Basel)] 2022 Nov 07; Vol. 12 (11). Date of Electronic Publication: 2022 Nov 07. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Pub Country of Publication: Switzerland NLM ID: 101609191 Publication Model: Electronic Cited Medium: Internet ISSN: 2079-6374 (Electronic) Linking ISSN: 20796374 NLM ISO Abbreviation: Biosensors (Basel) Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI Pub. |
مواضيع طبية MeSH: | Aptamers, Nucleotide* , Biosensing Techniques* , Graphite* , Nucleic Acids*, Thrombin/analysis ; Limit of Detection |
مستخلص: | Nucleic acid aptamer-based research has focused on achieving the highest performance for bioassays. However, there are limitations in evaluating the affinity for the target analytes in these nucleic acid aptamer-based bioassays. In this study, we mainly propose graphene oxide (GO)-based electrical and optical analyses to efficiently evaluate the affinity between an aptamer and its target. We found that an aptamer-coupled GO-based chip with an electrical resistance induced by a field-effect transistor, with aptamers as low as 100 pM, can detect the target, thrombin, at yields as low as 250 pM within five minutes. In the optical approach, the fluorescent dye-linked aptamer, as low as 100 nM, was efficiently used with GO, enabling the sensitive detection of thrombin at yields as low as 5 nM. The cantilever type of mechanical analysis also demonstrated the intuitive aptamer-thrombin reaction in the signal using dBm units. Finally, a comparison of electrical and optical sensors' characteristics was introduced in the attachment and detachment of aptamer to propose an efficient analysis that can be utilized for various aptamer-based research fields. |
References: | Anal Biochem. 2016 Sep 1;508:58-64. (PMID: 27318239) Anal Chim Acta. 2022 Apr 15;1202:339677. (PMID: 35341514) Biosens Bioelectron. 2016 Dec 15;86:764-769. (PMID: 27476058) Anal Chem. 2021 Oct 12;93(40):13673-13679. (PMID: 34597019) Chem Commun (Camb). 2014 Sep 18;50(72):10513-6. (PMID: 25072407) Sensors (Basel). 2018 May 28;18(6):. (PMID: 29843431) Anal Methods. 2020 Aug 14;12(30):3823-3835. (PMID: 32676627) J Biol Eng. 2020 Jan 13;14:1. (PMID: 31956340) Trends Biotechnol. 2011 May;29(5):205-12. (PMID: 21397350) Sci Rep. 2017 Mar 21;7(1):283. (PMID: 28325909) Nanoscale. 2012 Jan 7;4(1):143-7. (PMID: 22068751) Biochim Biophys Acta. 1973 May 17;310(1):289-94. (PMID: 4736336) Biosens Bioelectron. 2014 Oct 15;60:22-9. (PMID: 24768760) Appl Biochem Biotechnol. 2014 Jan;172(2):1018-26. (PMID: 24142359) Opt Express. 2017 Jan 9;25(1):39-58. (PMID: 28085810) Biosens Bioelectron. 2015 Feb 15;64:392-403. (PMID: 25278480) J Agric Food Chem. 2017 Mar 1;65(8):1771-1777. (PMID: 28161951) Biosens Bioelectron. 2019 Feb 1;126:88-95. (PMID: 30396022) Biosens Bioelectron. 2013 Oct 15;48:120-5. (PMID: 23665577) Biosens Bioelectron. 2013 Feb 15;40(1):24-31. (PMID: 22704840) Acta Naturae. 2013 Oct;5(4):34-43. (PMID: 24455181) Chem Commun (Camb). 2012 Feb 18;48(15):2071-3. (PMID: 22143382) Mikrochim Acta. 2018 Jun 28;185(7):344. (PMID: 29955964) Nanoscale. 2015 Jul 7;7(25):10903-7. (PMID: 25939390) Analyst. 2021 Jan 7;145(24):7932-7940. (PMID: 33025955) Fresenius J Anal Chem. 2000 Mar-Apr;366(6-7):540-51. (PMID: 11225766) Anal Chem. 2010 Mar 15;82(6):2341-6. (PMID: 20180560) Biosens Bioelectron. 2001 Jan;16(1-2):121-31. (PMID: 11261847) Arterioscler Thromb Vasc Biol. 2003 Jan 1;23(1):17-25. (PMID: 12524220) |
معلومات مُعتمدة: | 20015793 Ministry of Trade, Industry and Energy; NRF-2019R1A2C1087459 Ministry of Science ICT and Future Planning |
فهرسة مساهمة: | Keywords: aptamer; cantilever-integrated waveguide; field-effect transistor; fluorescence quenching; graphene oxide; thrombin detection |
المشرفين على المادة: | 0 (Aptamers, Nucleotide) EC 3.4.21.5 (Thrombin) 0 (graphene oxide) 7782-42-5 (Graphite) 0 (Nucleic Acids) |
تواريخ الأحداث: | Date Created: 20221110 Date Completed: 20221114 Latest Revision: 20221126 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC9688426 |
DOI: | 10.3390/bios12110979 |
PMID: | 36354488 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2079-6374 |
---|---|
DOI: | 10.3390/bios12110979 |