دورية أكاديمية
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Pathogenesis and Genomic Analysis of a Virulent Leptospira Interrogans Serovar Copenhageni Isolated from a Dog with Lethal Infection.

التفاصيل البيبلوغرافية
العنوان: Pathogenesis and Genomic Analysis of a Virulent Leptospira Interrogans Serovar Copenhageni Isolated from a Dog with Lethal Infection.
المؤلفون: de Oliveira NR; Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil., Kremer FS; Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil., de Brito RSA; Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil., Zamboni R; Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil., Dellagostin OA; Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil., Jorge S; Faculdade de Veterinária, Universidade Federal de Pelotas, Pelotas 96160-000, RS, Brazil.
المصدر: Tropical medicine and infectious disease [Trop Med Infect Dis] 2022 Oct 28; Vol. 7 (11). Date of Electronic Publication: 2022 Oct 28.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101709042 Publication Model: Electronic Cited Medium: Internet ISSN: 2414-6366 (Electronic) Linking ISSN: 24146366 NLM ISO Abbreviation: Trop Med Infect Dis Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2016]-
مستخلص: Dogs are highly susceptible to leptospirosis and are a public health concern due to their important role as a source of spreading disease, particularly in urban settings. In this study, we present the pathogenesis, serological characterization, and complete genome sequencing of a virulent Brazilian strain (NEG7) of L. interrogans serovar Copenhageni isolated from the urine of a dog that died due to acute leptospirosis. Clinical investigation showed that the dog was presented with icteric mucous membranes, weakness, dehydration, anorexia, and kidney and liver failures. Necropsy followed by histopathological evaluation revealed lesions compatible with liver and kidney leptospirosis. The leptospires recovered from the urine were further characterized by genome analysis, which confirmed that the isolate belonged to L. interrogans serogroup icterohaemorrhagiae serovar Copenhageni. Multiple bioinformatics tools were used to characterize the genomic features, and comparisons with other available Copenhageni strains were performed. Characterization based on absence of an INDEL in the gene lic12008, associated with phylogenetic and ANI (99.99% identity) analyses, confirmed the genetic relatedness of the isolate with L. interrogans serovar Copenhageni. A better understanding of the diversity of the pathogenic Leptospira isolates could help in identifying genotypes responsible for severe infections. Moreover, it can be used to develop control and prevention strategies for Leptospira serovars associated with particular animal reservoirs.
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فهرسة مساهمة: Keywords: bioinformatics; canine leptospirosis; infection; jaundice; whole-genome sequencing
تواريخ الأحداث: Date Created: 20221110 Latest Revision: 20221129
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9698576
DOI: 10.3390/tropicalmed7110333
PMID: 36355875
قاعدة البيانات: MEDLINE
الوصف
تدمد:2414-6366
DOI:10.3390/tropicalmed7110333