دورية أكاديمية
Mass spectrometry captures biased signalling and allosteric modulation of a G-protein-coupled receptor.
العنوان: | Mass spectrometry captures biased signalling and allosteric modulation of a G-protein-coupled receptor. |
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المؤلفون: | Yen HY; Chemical Research Laboratory, University of Oxford, Oxford, UK. hsinyungyen@gate.sinica.edu.tw.; OMass Therapeutics, Oxford, UK. hsinyungyen@gate.sinica.edu.tw.; Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan. hsinyungyen@gate.sinica.edu.tw., Liko I; Chemical Research Laboratory, University of Oxford, Oxford, UK.; OMass Therapeutics, Oxford, UK., Song W; Department of Biochemistry, University of Oxford, Oxford, UK.; Rahko, London, UK., Kapoor P; OMass Therapeutics, Oxford, UK., Almeida F; OMass Therapeutics, Oxford, UK., Toporowska J; Department of Chemistry, King's College London, London, UK., Gherbi K; OMass Therapeutics, Oxford, UK., Hopper JTS; OMass Therapeutics, Oxford, UK., Charlton SJ; OMass Therapeutics, Oxford, UK.; School of Life Sciences, Queen's Medical Centre, University of Nottingham, Nottingham, UK., Politis A; Department of Chemistry, King's College London, London, UK.; Faculty of Biology, Medicine and Health, School of Biological Sciences, The University of Manchester, Manchester, UK., Sansom MSP; Department of Biochemistry, University of Oxford, Oxford, UK., Jazayeri A; OMass Therapeutics, Oxford, UK. ali.jazayeri@omass.com., Robinson CV; Chemical Research Laboratory, University of Oxford, Oxford, UK. carol.robinson@chem.ox.ac.uk.; Kavli Institute for Nanoscience Discovery, Oxford, UK. carol.robinson@chem.ox.ac.uk. |
المصدر: | Nature chemistry [Nat Chem] 2022 Dec; Vol. 14 (12), pp. 1375-1382. Date of Electronic Publication: 2022 Nov 10. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101499734 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1755-4349 (Electronic) Linking ISSN: 17554330 NLM ISO Abbreviation: Nat Chem Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Nature Pub. Group |
مواضيع طبية MeSH: | Receptors, Adrenergic, beta-2*/chemistry , Receptors, Adrenergic, beta-2*/metabolism , Receptors, G-Protein-Coupled*/metabolism, Allosteric Regulation ; Isoproterenol/pharmacology ; Ligands ; GTP-Binding Proteins/metabolism ; Ions ; Mass Spectrometry ; Zinc/metabolism |
مستخلص: | G-protein-coupled receptors signal through cognate G proteins. Despite the widespread importance of these receptors, their regulatory mechanisms for G-protein selectivity are not fully understood. Here we present a native mass spectrometry-based approach to interrogate both biased signalling and allosteric modulation of the β (© 2022. The Author(s).) |
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معلومات مُعتمدة: | 221795 United Kingdom WT_ Wellcome Trust; 092970/Z/10/Z United Kingdom WT_ Wellcome Trust; BB/L002558/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; 092970 United Kingdom WT_ Wellcome Trust; United Kingdom WT_ Wellcome Trust; 221795/Z/20/Z United Kingdom WT_ Wellcome Trust |
سلسلة جزيئية: | figshare 10.6084/m9.figshare.19688640; 10.6084/m9.figshare.19754662.v1 |
المشرفين على المادة: | 0 (Receptors, Adrenergic, beta-2) L628TT009W (Isoproterenol) 0 (Receptors, G-Protein-Coupled) 0 (Ligands) EC 3.6.1.- (GTP-Binding Proteins) 0 (Ions) J41CSQ7QDS (Zinc) |
تواريخ الأحداث: | Date Created: 20221111 Date Completed: 20221220 Latest Revision: 20240706 |
رمز التحديث: | 20240706 |
مُعرف محوري في PubMed: | PMC9758051 |
DOI: | 10.1038/s41557-022-01041-9 |
PMID: | 36357787 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1755-4349 |
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DOI: | 10.1038/s41557-022-01041-9 |