دورية أكاديمية
أعرض في EDS

Tocotrienol-Rich Fraction and Levodopa Regulate Proteins Involved in Parkinson's Disease-Associated Pathways in Differentiated Neuroblastoma Cells: Insights from Quantitative Proteomic Analysis.

التفاصيل البيبلوغرافية
العنوان: Tocotrienol-Rich Fraction and Levodopa Regulate Proteins Involved in Parkinson's Disease-Associated Pathways in Differentiated Neuroblastoma Cells: Insights from Quantitative Proteomic Analysis.
المؤلفون: Magalingam KB; Jeffrey Cheah School of Medicine and Health Sciences, Monash University, Bandar Sunway 47500, Malaysia.; School of Postgraduate Studies, International Medical University, Kuala Lumpur 57000, Malaysia., Ramdas P; Division of Applied Biomedical Sciences and Biotechnology, School of Health Sciences, International Medical University, Kuala Lumpur 57000, Malaysia., Somanath SD; Pathology Division, School of Medicine, International Medical University, Kuala Lumpur 57000, Malaysia., Selvaduray KR; Product Development and Advisory Services Division, Malaysian Palm Oil Board, Bandar Baru Bangi 43000, Malaysia., Bhuvanendran S; Jeffrey Cheah School of Medicine and Health Sciences, Monash University, Bandar Sunway 47500, Malaysia., Radhakrishnan AK; Jeffrey Cheah School of Medicine and Health Sciences, Monash University, Bandar Sunway 47500, Malaysia.; Monash-Industry Palm Oil Education and Research Platform (MIPO), Monash University Malaysia, Bandar Sunway 47500, Malaysia.
المصدر: Nutrients [Nutrients] 2022 Nov 03; Vol. 14 (21). Date of Electronic Publication: 2022 Nov 03.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Publishing Country of Publication: Switzerland NLM ID: 101521595 Publication Model: Electronic Cited Medium: Internet ISSN: 2072-6643 (Electronic) Linking ISSN: 20726643 NLM ISO Abbreviation: Nutrients Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI Publishing
مواضيع طبية MeSH: Tocotrienols*/pharmacology , Tocotrienols*/therapeutic use , Parkinson Disease*/drug therapy , Neuroblastoma*/drug therapy, Humans ; Levodopa/pharmacology ; Proteomics ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Vitamin E
مستخلص: Tocotrienol-rich fraction (TRF), a palm oil-derived vitamin E fraction, is reported to possess potent neuroprotective effects. However, the modulation of proteomes in differentiated human neuroblastoma SH-SY5Y cells (diff-neural cells) by TRF has not yet been reported. This study aims to investigate the proteomic changes implicated by TRF in human neural cells using a label-free liquid-chromatography-double mass spectrometry (LC-MS/MS) approach. Levodopa, a drug used in the treatment of Parkinson's disease (PD), was used as a drug control. The human SH-SY5Y neuroblastoma cells were differentiated for six days and treated with TRF or levodopa for 24 h prior to quantitative proteomic analysis. A total of 81 and 57 proteins were differentially expressed in diff-neural cells following treatment with TRF or levodopa, respectively. Among these proteins, 32 similar proteins were detected in both TRF and levodopa-treated neural cells, with 30 of these proteins showing similar expression pattern. The pathway enrichment analysis revealed that most of the proteins regulated by TRF and levodopa are key players in the ubiquitin-proteasome, calcium signalling, protein processing in the endoplasmic reticulum, mitochondrial pathway and axonal transport system. In conclusion, TRF is an essential functional food that affects differential protein expression in human neuronal cells at the cellular and molecular levels.
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معلومات مُعتمدة: MU R 194 2016 Malaysian Palm Oil Board
فهرسة مساهمة: Keywords: Parkinson’s disease; SH-SY5Y neuroblastoma cells; proteomics; tocotrienol-rich fraction (TRF)
المشرفين على المادة: 0 (Tocotrienols)
46627O600J (Levodopa)
1406-18-4 (Vitamin E)
تواريخ الأحداث: Date Created: 20221111 Date Completed: 20221114 Latest Revision: 20221117
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9658582
DOI: 10.3390/nu14214632
PMID: 36364894
قاعدة البيانات: MEDLINE
الوصف
تدمد:2072-6643
DOI:10.3390/nu14214632