دورية أكاديمية
Macrophage In Vitro and In Vivo Tracking via Anchored Microcapsules.
| العنوان: | Macrophage In Vitro and In Vivo Tracking via Anchored Microcapsules. |
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| المؤلفون: | Sapach AY; Skolkovo Institute of Science and Technology, Moscow 143005, Russia.; Sechenov First State Medical University, Moscow 119991, Russia., Sindeeva OA; Skolkovo Institute of Science and Technology, Moscow 143005, Russia., Nesterchuk MV; Skolkovo Institute of Science and Technology, Moscow 143005, Russia., Tsitrina AA; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow 119334, Russia., Mayorova OA; Saratov State University, Saratov 410012, Russia., Prikhozhdenko ES; Saratov State University, Saratov 410012, Russia., Verkhovskii RA; Saratov State University, Saratov 410012, Russia., Mikaelyan AS; Koltzov Institute of Developmental Biology of Russian Academy of Sciences, Moscow 119334, Russia., Kotelevtsev YV; Skolkovo Institute of Science and Technology, Moscow 143005, Russia., Sukhorukov GB; Skolkovo Institute of Science and Technology, Moscow 143005, Russia.; Siberian State Medical University, Tomsk 634050, Russia.; Queen Mary University of London, London E1 4NS, U.K. |
| المصدر: | ACS applied materials & interfaces [ACS Appl Mater Interfaces] 2022 Nov 23; Vol. 14 (46), pp. 51579-51592. Date of Electronic Publication: 2022 Nov 11. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101504991 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1944-8252 (Electronic) Linking ISSN: 19448244 NLM ISO Abbreviation: ACS Appl Mater Interfaces Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society |
| مواضيع طبية MeSH: | Drug Carriers* , Drug Delivery Systems*, Capsules ; Macrophages ; Cell Tracking |
| مستخلص: | A new promising trend in personalized medicine is the use of autologous cells (macrophages or stem cells) for cell-based therapy and also as a "Trojan horse" for targeted delivery of a drug carrier. The natural ability of macrophages for chemotaxis allows them to deliver cargo to the damaged area, significantly reducing side effects on healthy organ tissues. Therefore, it is important to develop tools to track their behavior in the organism. While labeled containers can serve as anchored tags for imaging macrophages in vivo , they can affect the properties and functions of macrophages. This work demonstrates that 3 μm sized capsules based on biocompatible polyelectrolytes and fluorescently labeled with both Cy7 and RITC dyes do not affect cell functionalization in vitro , such as viability, proliferation, and movement of transformed monocyte/macrophage-like cells (RAW 264.7) and primary bone marrow derived macrophages (BMDM) at maximal loading of five capsules per cell. In addition, capsules allowed fluorescent detection of ex vivo loaded cells 24 h after the tail vein injection in vivo and visualization of microcapsule-laden macrophages ex vivo using confocal microscopy. We have delivered about 62.5% of injected BMDM containing 12.5 million capsules with 3.75 μg of high-molecular-weight cargo (0.3 pg/capsule) to the liver. Our results demonstrate that 3 μm polyelectrolyte fluorescently labeled microcapsules can be used for safe macrophage loading, allowing cell tracking and drug delivery, which will facilitate development of macrophage-based cell therapy protocols. |
| فهرسة مساهمة: | Keywords: cell tracking; fluorescent label; macrophage-mediated drug delivery system; macrophages; microcapsule-laden macrophages; microcapsules; nanomaterials |
| المشرفين على المادة: | 0 (Capsules) 0 (Drug Carriers) |
| تواريخ الأحداث: | Date Created: 20221111 Date Completed: 20221124 Latest Revision: 20221124 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1021/acsami.2c12004 |
| PMID: | 36367877 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1944-8252 |
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| DOI: | 10.1021/acsami.2c12004 |