دورية أكاديمية
Utilization of novel lectin-conjugated Au nanoparticles as Thomsen-Friedenreich onco-antigen target for in vitro cytotoxicity and apoptosis induction in leukemic cell line.
| العنوان: | Utilization of novel lectin-conjugated Au nanoparticles as Thomsen-Friedenreich onco-antigen target for in vitro cytotoxicity and apoptosis induction in leukemic cell line. |
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| المؤلفون: | Akram SM; Al-Nahrain University, College of Biotechnology, Dept. of Molecular and Medical Biotechnology, Baghdad, Iraq., Al-Saffar AZ; Al-Nahrain University, College of Biotechnology, Dept. of Molecular and Medical Biotechnology, Baghdad, Iraq. Electronic address: ali.saffar@nahrainuniv.edu.iq., Hadi NA; Al-Nahrain University, College of Biotechnology, Dept. of Plant Biotechnology, Baghdad, Iraq. Electronic address: noora.adil@nahrainuniv.edu.iq., Akram SM; Ashur University College, Dept. of Medical Laboratory Techniques, Baghdad, Iraq. |
| المصدر: | Life sciences [Life Sci] 2022 Dec 15; Vol. 311 (Pt A), pp. 121163. Date of Electronic Publication: 2022 Nov 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2008->: Amsterdam : Elsevier Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Gold*/pharmacology , Gold*/chemistry , Metal Nanoparticles*/chemistry, Humans ; Lectins/pharmacology ; Molecular Docking Simulation ; Apoptosis ; HL-60 Cells ; Cell Line, Tumor |
| مستخلص: | Leukemia is a tumor of blood-forming tissues including bone marrow and lymphatic nodes, which comprise biologically distinct subgroups. In the present study, Au NPs-PEG-Lectin was prepared as a drug targeting system for potential Thomsen-Friedenreich antigen (TF-Ag) presented on the surface of leukemic cells to induce cytotoxicity. Gold nanoparticles were prepared using citrate reduction method and conjugated with lectin via SH-PEG-COOH. The conjugate was characterized using UV/Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), Zeta potential, and Scanning electron microscopy (SEM) with subsequent applications for cytotoxicity, cell cycle analysis, and apoptosis. Immunophenotypically blood samples from patients with acute lymphoblastic leukemia (ALL) were positively expressed CD45, CD95 dim expression, and low CD176 (TF-Ag) expression. Samples of acute myeloid leukemia (AML) confirmed the expression of all markers. Au NPs-PEG-Lectin conjugate showed an average size of 35.82 nm with zeta potential of -27.33 with accelerated lectin release from the conjugate at acidic pH. Au NPs-PEG-Lectin demonstrated the highest and most significant cytotoxic activity against HL-60 and K562 with IC Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest that might influence the publication of this article. (Copyright © 2022 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Apoptosis; Au NPs; Cytotoxicity; Lectin; Leukemia; Thomsen-Friedenreich antigen (TF-Ag) |
| المشرفين على المادة: | 7440-57-5 (Gold) 3554-90-3 (Thomsen-Friedenreich antigen) 0 (Lectins) |
| تواريخ الأحداث: | Date Created: 20221111 Date Completed: 20221129 Latest Revision: 20221129 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.lfs.2022.121163 |
| PMID: | 36368415 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0631 |
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| DOI: | 10.1016/j.lfs.2022.121163 |