دورية أكاديمية
A dual-labeled fluorescent probe for visualization of dextranase activity in a simulated food digestion system.
العنوان: | A dual-labeled fluorescent probe for visualization of dextranase activity in a simulated food digestion system. |
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المؤلفون: | Sun R; Life Quality Engineering Interest Group, School of Chemical and Environmental Engineering, Soochow University, Suzhou Industrial Park Campus, Jiangsu Province 215123, China., Liu W; Life Quality Engineering Interest Group, School of Chemical and Environmental Engineering, Soochow University, Suzhou Industrial Park Campus, Jiangsu Province 215123, China., Kirk TV; Life Quality Engineering Interest Group, School of Chemical and Environmental Engineering, Soochow University, Suzhou Industrial Park Campus, Jiangsu Province 215123, China. Electronic address: tim@suda.edu.cn., Chen XD; Life Quality Engineering Interest Group, School of Chemical and Environmental Engineering, Soochow University, Suzhou Industrial Park Campus, Jiangsu Province 215123, China. Electronic address: xdchen@mail.suda.edu.cn. |
المصدر: | Food chemistry [Food Chem] 2023 Mar 30; Vol. 405 (Pt A), pp. 134744. Date of Electronic Publication: 2022 Oct 29. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Barking : Elsevier Applied Science Publishers Original Publication: Barking, Eng., Applied Science Publishers. |
مواضيع طبية MeSH: | Dextranase* , Fluorescent Dyes*, Biotin ; Sepharose ; Digestion |
مستخلص: | Molecular bioimaging of enzyme activity is rapidly emerging as a powerful strategy for accurate disease diagnostics. This work aims to prove that bioimaging of enzyme activity in food digestion with a fluorescent probe is feasible. In this study, a dual-labeled fluorescent probe with dextran-tetramethylrhodamine (TMR)-biotin conjugate (DTB) as the enzyme-cleavable unit, and biotin-(5-fluorescein) conjugate (FB) as the reference unit, was developed. It was immobilized in the agarose gel (the model food matrix) for the fluorescence quantification of dextranase activity. The probe manifested significantly ratiometric fluorescent signals (I Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022 Elsevier Ltd. All rights reserved.) |
فهرسة مساهمة: | Keywords: Diffusion; Enzyme imaging; Fluorescent probe; Food digestion; Ratiometric |
المشرفين على المادة: | EC 3.2.1.11 (Dextranase) 0 (Fluorescent Dyes) 6SO6U10H04 (Biotin) 9012-36-6 (Sepharose) |
تواريخ الأحداث: | Date Created: 20221113 Date Completed: 20221207 Latest Revision: 20221207 |
رمز التحديث: | 20231215 |
DOI: | 10.1016/j.foodchem.2022.134744 |
PMID: | 36371837 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1873-7072 |
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DOI: | 10.1016/j.foodchem.2022.134744 |