دورية أكاديمية
أعرض في EDS

A dual-labeled fluorescent probe for visualization of dextranase activity in a simulated food digestion system.

التفاصيل البيبلوغرافية
العنوان: A dual-labeled fluorescent probe for visualization of dextranase activity in a simulated food digestion system.
المؤلفون: Sun R; Life Quality Engineering Interest Group, School of Chemical and Environmental Engineering, Soochow University, Suzhou Industrial Park Campus, Jiangsu Province 215123, China., Liu W; Life Quality Engineering Interest Group, School of Chemical and Environmental Engineering, Soochow University, Suzhou Industrial Park Campus, Jiangsu Province 215123, China., Kirk TV; Life Quality Engineering Interest Group, School of Chemical and Environmental Engineering, Soochow University, Suzhou Industrial Park Campus, Jiangsu Province 215123, China. Electronic address: tim@suda.edu.cn., Chen XD; Life Quality Engineering Interest Group, School of Chemical and Environmental Engineering, Soochow University, Suzhou Industrial Park Campus, Jiangsu Province 215123, China. Electronic address: xdchen@mail.suda.edu.cn.
المصدر: Food chemistry [Food Chem] 2023 Mar 30; Vol. 405 (Pt A), pp. 134744. Date of Electronic Publication: 2022 Oct 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE
أسماء مطبوعة: Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
مواضيع طبية MeSH: Dextranase* , Fluorescent Dyes*, Biotin ; Sepharose ; Digestion
مستخلص: Molecular bioimaging of enzyme activity is rapidly emerging as a powerful strategy for accurate disease diagnostics. This work aims to prove that bioimaging of enzyme activity in food digestion with a fluorescent probe is feasible. In this study, a dual-labeled fluorescent probe with dextran-tetramethylrhodamine (TMR)-biotin conjugate (DTB) as the enzyme-cleavable unit, and biotin-(5-fluorescein) conjugate (FB) as the reference unit, was developed. It was immobilized in the agarose gel (the model food matrix) for the fluorescence quantification of dextranase activity. The probe manifested significantly ratiometric fluorescent signals (I green /I red ) in response to the enzyme-active reaction. Linear relationships of I green /I red were obtained against the dextranase concentration ratio (C/C 0 ). I green /I red increased more rapidly with a greater dextranase diffusion rate, also supported by the more significant diffusion coefficient of fluorescently labeled dextranase in 0.5 wt% agarose gel (1.87 × 10 -6 cm 2 s -1 ). Our work provides more mechanistic evidence for enzyme activity imaging in food digestion.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Diffusion; Enzyme imaging; Fluorescent probe; Food digestion; Ratiometric
المشرفين على المادة: EC 3.2.1.11 (Dextranase)
0 (Fluorescent Dyes)
6SO6U10H04 (Biotin)
9012-36-6 (Sepharose)
تواريخ الأحداث: Date Created: 20221113 Date Completed: 20221207 Latest Revision: 20221207
رمز التحديث: 20231215
DOI: 10.1016/j.foodchem.2022.134744
PMID: 36371837
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-7072
DOI:10.1016/j.foodchem.2022.134744