دورية أكاديمية
أعرض في EDS

Diagnostic testing of chronic wasting disease in white-tailed deer (Odocoileus virginianus) by RT-QuIC using multiple tissues.

التفاصيل البيبلوغرافية
العنوان: Diagnostic testing of chronic wasting disease in white-tailed deer (Odocoileus virginianus) by RT-QuIC using multiple tissues.
المؤلفون: Burgener KR; Molecular and Environmental Toxicology Program, University of Wisconsin-Madison, Madison, Wisconsin, United Sates of America., Lichtenberg SS; Department of Soil Science, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Lomax A; Department of Soil Science, University of Wisconsin-Madison, Madison, Wisconsin, United States of America., Storm DJ; Wisconsin Department of Natural Resources, Eau Claire, Wisconsin, United States of America., Walsh DP; U.S. Geological Survey, National Wildlife Health Center, Madison, Wisconsin, United States of America., Pedersen JA; Molecular and Environmental Toxicology Program, University of Wisconsin-Madison, Madison, Wisconsin, United Sates of America.; Department of Soil Science, University of Wisconsin-Madison, Madison, Wisconsin, United States of America.
المصدر: PloS one [PLoS One] 2022 Nov 16; Vol. 17 (11), pp. e0274531. Date of Electronic Publication: 2022 Nov 16 (Print Publication: 2022).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Wasting Disease, Chronic*/diagnosis , Wasting Disease, Chronic*/pathology , Deer* , Prions*, Animals ; Biopsy
مستخلص: Chronic wasting disease (CWD) is a fatal prion disease affecting cervids (deer, elk, moose). Current methods to monitor individual disease state include highly invasive antemortem rectal biopsy or postmortem brain biopsy. Efficient, sensitive, and selective antemortem and postmortem testing of populations would increase knowledge of the dynamics of CWD epizootics as well as provide a means to track CWD progression into previously unaffected areas. Here, we analyzed the presence of CWD prions in skin samples from two easily accessed locations (ear and belly) from 30 deceased white-tailed deer (Odocoileus viginianus). The skin samples were enzymatically digested and analyzed by real-time quaking-induced conversion (RT-QuIC). The diagnostic sensitivity of the ear and belly skin samples were both 95%, and the diagnostic specificity of the ear and belly skin were both 100%. Additionally, the location of the skin biopsy on the ear does not affect specificity or sensitivity. These results demonstrate the efficacy of CWD diagnosis with skin biopsies using RT-QuIC. This method could be useful for large scale antemortem population testing.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.)
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المشرفين على المادة: 0 (Prions)
SCR Organism: Odocoileus virginianus
تواريخ الأحداث: Date Created: 20221116 Date Completed: 20221118 Latest Revision: 20221121
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC9668146
DOI: 10.1371/journal.pone.0274531
PMID: 36383520
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0274531