دورية أكاديمية
أعرض في EDS

ROS scavenging of SOD/CAT mimics probed by EPR and reduction of lipid peroxidation in S. cerevisiae and mouse liver, under severe hydroxyl radical stress condition.

التفاصيل البيبلوغرافية
العنوان: ROS scavenging of SOD/CAT mimics probed by EPR and reduction of lipid peroxidation in S. cerevisiae and mouse liver, under severe hydroxyl radical stress condition.
المؤلفون: Menezes LB; Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil., Segat BB; Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil., Tolentino H; LABOX, Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil., Pires DC; Rede Micologia RJ- FAPERJ; Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, RJ, Brazil., Mattos LMM; Rede Micologia RJ- FAPERJ; Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, RJ, Brazil., Hottum HM; Rede Micologia RJ- FAPERJ; Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, RJ, Brazil., Pereira MD; Rede Micologia RJ- FAPERJ; Departamento de Bioquímica, Instituto de Química, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-909, RJ, Brazil., Latini A; LABOX, Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil., Horn A Jr; Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil. Electronic address: adolfo.junior@ufsc.br., Fernandes C; Departamento de Química, Universidade Federal de Santa Catarina, Florianópolis 88040-900, SC, Brazil. Electronic address: christiane.horn@ufsc.br.
المصدر: Journal of inorganic biochemistry [J Inorg Biochem] 2023 Feb; Vol. 239, pp. 112062. Date of Electronic Publication: 2022 Nov 05.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: United States NLM ID: 7905788 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3344 (Electronic) Linking ISSN: 01620134 NLM ISO Abbreviation: J Inorg Biochem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, Elsevier.
مواضيع طبية MeSH: Saccharomyces cerevisiae*/metabolism , Superoxides*, Mice ; Animals ; Lipid Peroxidation ; Reactive Oxygen Species ; Hydroxyl Radical ; Copper/chemistry ; Ferric Compounds ; Hydrogen Peroxide ; Free Radicals ; Superoxide Dismutase/metabolism ; Liver/metabolism ; Pyridines
مستخلص: The interaction between Cu II , Fe III and Mn II complexes, derived from the ligands 1-[bis(pyridine-2-ylmethyl)amino]-3-chloropropan-2-ol (hpclnol) and bis(pyridine-2-ylmethyl)amine (bpma), and the free radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl (DPPH) and reactive oxygen species (ROS), was investigated by colorimetric and EPR (Electron Paramagnetic Resonance) techniques. A comparison between these results and those reported to [Mn(salen)Cl] or EUK-8 was also addressed. EPR studies allowed us the identification of intermediates species such as superoxide‑copper(I) and superoxide‑copper(II), a mixed-valence Fe III Fe II species and a 16-line feature attributed to Mn III -oxo-Mn IV species. The biomarker malondialdehyde (MDA) was determined by TBARS assay in S. cerevisiae cells, and the determination of the IC 50 indicate that the antioxidant activity shown dependence on the metal center (Cu II  ≈ Fe III  > Mn II  ≈ [Mn(salen)Cl]. The lipid peroxidation attenuation was also investigated in liver homogenates obtained from Swiss mice and the IC 50 values were in the nanomolar concentrations. We demonstrated here that all the complexes interact with the free radical DPPH and with ROS (H 2 O 2 , O 2 •- and hydroxyl radical), enhancing the cellular protection against oxidative stress generated by hydroxyl radical, employing two experimental model systems, S. cerevisiae (in vivo) and mouse liver (ex vivo).
Competing Interests: Declaration of Competing Interest The authors declare that they have no any conflict of interest.
(Copyright © 2022. Published by Elsevier Inc.)
فهرسة مساهمة: Keywords: EPR; Lipid peroxidation; Mouse liver homogenate; ROS; S. cerevisiae; SOD and CAT mimics
المشرفين على المادة: 94-93-9 (disalicylaldehyde ethylenediamine)
0 (Reactive Oxygen Species)
11062-77-4 (Superoxides)
3352-57-6 (Hydroxyl Radical)
789U1901C5 (Copper)
0 (Ferric Compounds)
BBX060AN9V (Hydrogen Peroxide)
0 (Free Radicals)
EC 1.15.1.1 (Superoxide Dismutase)
0 (Pyridines)
تواريخ الأحداث: Date Created: 20221120 Date Completed: 20221226 Latest Revision: 20230223
رمز التحديث: 20240628
DOI: 10.1016/j.jinorgbio.2022.112062
PMID: 36403436
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-3344
DOI:10.1016/j.jinorgbio.2022.112062