دورية أكاديمية
أعرض في EDS

Plasma metabolomics and gene regulatory networks analysis reveal the role of nonstructural SARS-CoV-2 viral proteins in metabolic dysregulation in COVID-19 patients.

التفاصيل البيبلوغرافية
العنوان: Plasma metabolomics and gene regulatory networks analysis reveal the role of nonstructural SARS-CoV-2 viral proteins in metabolic dysregulation in COVID-19 patients.
المؤلفون: Ivanisenko VA; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia. salix@bionet.nsc.ru.; Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia. salix@bionet.nsc.ru.; Kurchatov Genomics Center of Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia. salix@bionet.nsc.ru., Gaisler EV; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia., Basov NV; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia., Rogachev AD; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.; N. N. Vorozhtsov, Novosibirsk Institute of Organic Chemistry, Acad. Lavrentiev Ave., 9, 630090, Novosibirsk, Russia., Cheresiz SV; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia., Ivanisenko TV; Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia.; Kurchatov Genomics Center of Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia., Demenkov PS; Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia.; Kurchatov Genomics Center of Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia., Mishchenko EL; Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia., Khripko OP; Federal Research Center for Fundamental and Translational Medicine, Timakov Str., 2, 630117, Novosibirsk, Russia., Khripko YI; Federal Research Center for Fundamental and Translational Medicine, Timakov Str., 2, 630117, Novosibirsk, Russia., Voevoda SM; Federal Research Center for Fundamental and Translational Medicine, Timakov Str., 2, 630117, Novosibirsk, Russia.; Research Institute of Internal and Preventive Medicine-Branch of the Institute of Cytology and Genetics, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia., Karpenko TN; State Budgetary Healthcare Institution of Novosibirsk Region 'City Clinical Hospital No. 11', Tankistov str., 23, 630120, Novosibirsk, Russia., Velichko AJ; State Budgetary Healthcare Institution of Novosibirsk Region 'City Clinical Hospital No. 11', Tankistov str., 23, 630120, Novosibirsk, Russia., Voevoda MI; Federal Research Center for Fundamental and Translational Medicine, Timakov Str., 2, 630117, Novosibirsk, Russia., Kolchanov NA; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.; Institute of Cytology and Genetics of Siberian Branch of Russian Academy of Sciences, Acad. Lavrentiev Ave., 10, 630090, Novosibirsk, Russia., Pokrovsky AG; Novosibirsk State University, Pirogova Str., 2, 630090, Novosibirsk, Russia.
المصدر: Scientific reports [Sci Rep] 2022 Nov 20; Vol. 12 (1), pp. 19977. Date of Electronic Publication: 2022 Nov 20.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: SARS-CoV-2*/genetics , COVID-19*, Humans ; Gene Regulatory Networks ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Plasma ; Viral Proteins/genetics ; Disease Progression
مستخلص: Metabolomic analysis of blood plasma samples from COVID-19 patients is a promising approach allowing for the evaluation of disease progression. We performed the metabolomic analysis of plasma samples of 30 COVID-19 patients and the 19 controls using the high-performance liquid chromatography (HPLC) coupled with tandem mass spectrometric detection (LC-MS/MS). In our analysis, we identified 103 metabolites enriched in KEGG metabolic pathways such as amino acid metabolism and the biosynthesis of aminoacyl-tRNAs, which differed significantly between the COVID-19 patients and the controls. Using ANDSystem software, we performed the reconstruction of gene networks describing the potential genetic regulation of metabolic pathways perturbed in COVID-19 patients by SARS-CoV-2 proteins. The nonstructural proteins of SARS-CoV-2 (orf8 and nsp5) and structural protein E were involved in the greater number of regulatory pathways. The reconstructed gene networks suggest the hypotheses on the molecular mechanisms of virus-host interactions in COVID-19 pathology and provide a basis for the further experimental and computer studies of the regulation of metabolic pathways by SARS-CoV-2 proteins. Our metabolomic analysis suggests the need for nonstructural protein-based vaccines and the control strategy to reduce the disease progression of COVID-19.
(© 2022. The Author(s).)
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معلومات مُعتمدة: FWNR-2022-0020 Ministry of Science and Higher Education of the Russian Federation; FSUS-2020-0035 Ministry of Science and Higher Education of the Russian Federation; 075-15-2021-944 Ministry of Science and Higher Education of the Russian Federation; FSUS-2020-0035 Ministry of Science and Higher Education of the Russian Federation; 20-04-60314 Russian Foundation for Basic Research
المشرفين على المادة: 0 (Viral Proteins)
تواريخ الأحداث: Date Created: 20221120 Date Completed: 20221122 Latest Revision: 20230110
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9676188
DOI: 10.1038/s41598-022-24170-0
PMID: 36404352
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-022-24170-0