دورية أكاديمية
Prevalence of extended spectrum Beta-Lactamase producing Klebsiella species from patients' specimens in a tertiary teaching hospital in Ile-Ife, Southwest Nigeria.
| العنوان: | Prevalence of extended spectrum Beta-Lactamase producing Klebsiella species from patients' specimens in a tertiary teaching hospital in Ile-Ife, Southwest Nigeria. |
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| المؤلفون: | Ajimuda OE; Department of Medical Microbiology and Parasitology, Obafemi Awolowo University, Ile-Ife, Nigeria., Sanmi-Kayode I; Department of Medical Microbiology and Parasitology, Obafemi Awolowo University, Ile-Ife, Nigeria., Adeniyi OO; Department of Medical Microbiology and Parasitology, Obafemi Awolowo University, Ile-Ife, Nigeria., Alaka OO; Department of Medical Microbiology and Parasitology, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria., Onipede A; Department of Medical Microbiology and Parasitology, Obafemi Awolowo University, Ile-Ife, Nigeria.; Department of Medical Microbiology and Parasitology, Obafemi Awolowo University Teaching Hospitals Complex, Ile-Ife, Nigeria. |
| المصدر: | African health sciences [Afr Health Sci] 2022 Jun; Vol. 22 (2), pp. 146-155. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Faculty of Medicine, Makerere University Country of Publication: Uganda NLM ID: 101149451 Publication Model: Print Cited Medium: Internet ISSN: 1729-0503 (Electronic) Linking ISSN: 16806905 NLM ISO Abbreviation: Afr Health Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Kampala, Uganda : Faculty of Medicine, Makerere University, [2001- |
| مواضيع طبية MeSH: | beta-Lactamases*/genetics , Klebsiella*/genetics, Humans ; Prevalence ; Cross-Sectional Studies ; Nigeria/epidemiology ; Hospitals, Teaching |
| مستخلص: | Objective: This study determined the prevalence of ESBL genes amongst Klebsiella species isolated from patients' specimens attending a Tertiary Teaching Hospital, in Ile-Ife, Southwest Nigeria. Methods: A cross sectional study of presumptive isolates of Klebsiella (n=180) were collected, after ethical approval in the microbiology laboratory. Isolates were identified to species level by conventional biochemical tests and MicrobactTM 24E Identification Kits. Antibiotic susceptibility testing was performed by the Kirby Bauer's disk diffusion method. ESBL production was detected by the double disc synergy and ESBL genes by the multiplex PCR protocol. Results: The Klebsiella species identified were Klebsiella pneumoniae 95.6%, Klebsiella oxytoca 3.3%, Klebsiella ornithinolytica 0.6% and Klebsiella terrigena 0.6%. The prevalence of ESBL genes among the Klebsiella isolates was 47.2%, and the most common ESBL gene was blaSHV (38.9%), also 75% of the study isolates had MAR index greater than 0.2. Conclusions: The study establishes the prevalence of Extended Spectrum Beta-Lactamase producing Klebsiella sp in the hospital and identified the most prevalent ESBL genes circulating as blaSHV followed by blaTEM and blaCTX-M in this environment. This underscores the need for regular and continuous surveillance of antimicrobial resistance trend as a strong component of the antibiotics stewardship and infection prevention and control programs in the hospital. (© 2022 Ajimuda OE et al.) |
| References: | BMC Res Notes. 2018 Jul 13;11(1):471. (PMID: 30005695) Microbiologyopen. 2019 Sep;8(9):e00816. (PMID: 30801967) Jundishapur J Microbiol. 2014 Feb;7(2):e8756. (PMID: 25147671) Clin Infect Dis. 2015 May 1;60(9):1319-25. (PMID: 25586681) Afr Health Sci. 2013 Dec;13(4):1091-7. (PMID: 24940337) J Clin Diagn Res. 2013 Sep;7(9):1975-8. (PMID: 24179913) Antimicrob Agents Chemother. 2012 Apr;56(4):2108-13. (PMID: 22252816) Clin Infect Dis. 2014 Jan;58(2):225-32. (PMID: 24099919) Perspect Medicin Chem. 2014 Aug 28;6:25-64. (PMID: 25232278) Antimicrob Agents Chemother. 2012 Mar;56(3):1609-12. (PMID: 22155836) Int J Med Microbiol. 2010 Aug;300(6):371-9. (PMID: 20537585) Int J Microbiol. 2017;2017:8486742. (PMID: 28250772) Bosn J Basic Med Sci. 2011 May;11(2):91-6. (PMID: 21619555) J Clin Diagn Res. 2016 Feb;10(2):DC07-10. (PMID: 27042460) J Infect Dev Ctries. 2010 Nov 24;4(11):717-22. (PMID: 21252449) |
| فهرسة مساهمة: | Keywords: Antibiotic Resistance; ESBL; Klebsiella |
| المشرفين على المادة: | EC 3.5.2.6 (beta-Lactamases) |
| تواريخ الأحداث: | Date Created: 20221121 Date Completed: 20221122 Latest Revision: 20221122 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9652647 |
| DOI: | 10.4314/ahs.v22i2.17 |
| PMID: | 36407350 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1729-0503 |
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| DOI: | 10.4314/ahs.v22i2.17 |