دورية أكاديمية
أعرض في EDS

Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA.

التفاصيل البيبلوغرافية
العنوان: Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA.
المؤلفون: Husser MC; Biology Department, Concordia University, Montreal, Quebec, Canada., Ozugergin I; Biology Department, Concordia University, Montreal, Quebec, Canada., Resta T; Biology Department, Concordia University, Montreal, Quebec, Canada., Martin VJJ; Biology Department, Concordia University, Montreal, Quebec, Canada.; Center for Applied Synthetic Biology, Concordia University, Montreal, Quebec, Canada., Piekny AJ; Biology Department, Concordia University, Montreal, Quebec, Canada.; Center for Applied Synthetic Biology, Concordia University, Montreal, Quebec, Canada.; Center for Microscopy and Cellular Imaging, Concordia University, Montreal, Quebec, Canada.
المصدر: Open biology [Open Biol] 2022 Nov; Vol. 12 (11), pp. 220247. Date of Electronic Publication: 2022 Nov 23.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Royal Society Pub Country of Publication: England NLM ID: 101580419 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2046-2441 (Electronic) Linking ISSN: 20462441 NLM ISO Abbreviation: Open Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Royal Society Pub., [2011]-
مواضيع طبية MeSH: Actomyosin*/metabolism , Contractile Proteins*/genetics , Contractile Proteins*/metabolism , Cytokinesis* , Proto-Oncogene Proteins*/genetics , Proto-Oncogene Proteins*/metabolism , rhoA GTP-Binding Protein*/genetics , rhoA GTP-Binding Protein*/metabolism, Humans ; HeLa Cells
مستخلص: Cytokinesis is required to physically separate the daughter cells at the end of mitosis. This crucial process requires the assembly and ingression of an actomyosin ring, which must occur with high fidelity to avoid aneuploidy and cell fate changes. Most of our knowledge of mammalian cytokinesis was generated using over-expressed transgenes in HeLa cells. Over-expression can introduce artefacts, while HeLa are cancerous human cells that have lost their epithelial identity, and the mechanisms controlling cytokinesis in these cells could be vastly different from other cell types. Here, we tagged endogenous anillin, Ect2 and RhoA with mNeonGreen and characterized their localization during cytokinesis for the first time in live human cells. Comparing anillin localization in multiple cell types revealed cytokinetic diversity with differences in the duration and symmetry of ring closure, and the timing of cortical recruitment. Our findings show that the breadth of anillin correlates with the rate of ring closure, and support models where cell size or ploidy affects the cortical organization, and intrinsic mechanisms control the symmetry of ring closure. This work highlights the need to study cytokinesis in more diverse cell types, which will be facilitated by the reagents generated for this study.
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فهرسة مساهمة: Keywords: CRISPR; RhoA; actomyosin; cytokinesis; microscopy
المشرفين على المادة: 9013-26-7 (Actomyosin)
0 (anillin)
0 (Contractile Proteins)
0 (ECT2 protein, human)
0 (Proto-Oncogene Proteins)
EC 3.6.5.2 (rhoA GTP-Binding Protein)
124671-05-2 (RHOA protein, human)
تواريخ الأحداث: Date Created: 20221123 Date Completed: 20221128 Latest Revision: 20230418
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9683116
DOI: 10.1098/rsob.220247
PMID: 36416720
قاعدة البيانات: MEDLINE
الوصف
تدمد:2046-2441
DOI:10.1098/rsob.220247