دورية أكاديمية
Bacterial Interactions as Detected by Pooled Antibiotic Susceptibility Testing (P-AST) in Polymicrobial Urine Specimens.
| العنوان: | Bacterial Interactions as Detected by Pooled Antibiotic Susceptibility Testing (P-AST) in Polymicrobial Urine Specimens. |
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| المؤلفون: | Vollstedt A; Beaumont Hospital, 3601 W. Thirteen Mile Rd, Royal Oak, MI 48073, USA., Baunoch D; Pathnostics, 17661 Cowan, Irvine, CA 92614, USA., Wolfe A; Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL, USA., Luke N; Pathnostics, 17661 Cowan, Irvine, CA 92614, USA., Wojno KJ; Comprehensive Urology-A Division of Michigan Healthcare Professionals, 31157 Woodward Ave, Royal Oak, MI 48073, USA., Cline K; Regional Urology, LLC, 255 Bert Kouns, Shreveport, LA 71106, USA., Belkoff L; Urologic Consultants of SE PA, 1 Presidential Blvd, Suite 115, Bala Cynwyd, PA 19004, USA., Milbank A; Minnesota Urology, 11850 Blackfoot Street NW, Suite #470, Coon Rapids, MN 55125, USA., Sherman N; Premier Urology, 10 Parsonage Road, Suite # 118, Edison, NJ, 08837, USA., Haverkorn R; Urology San Antonio, 7909 Fredericksburg Rd, Suite 150, San Antonio, TX, 78229, USA., Gaines N; Urology San Antonio, 7909 Fredericksburg Rd, Suite 150, San Antonio, TX, 78229, USA., Yore L; Advanced Urology of SO FL, LLC, 5350 W Atlantic Blvd, #102, Delray Beach, FL 33484, USA., Shore N; Carolina Urologic Research Center, 823 82nd Parkway, Suite B, Myrtle Beach, SC 29572, USA., Opel M; Pathnostics, 17661 Cowan, Irvine, CA 92614, USA., Korman H; Comprehensive Urology-A Division of Michigan Healthcare Professionals, 31157 Woodward Ave, Royal Oak, MI 48073, USA., Kelly C; Kelly Statistical Consulting, Palomar Airport Rd, Carlsbad, CA 92011, USA., Jafri M; Comprehensive Urology-A Division of Michigan Healthcare Professionals, 31157 Woodward Ave, Royal Oak, MI 48073, USA., Campbell M; Pathnostics, 17661 Cowan, Irvine, CA 92614, USA.; University of Kentucky College of Medicine, 800 Rose Street MN 150, Lexington, KY 40506, USA., Keating P; Comprehensive Urology-A Division of Michigan Healthcare Professionals, 31157 Woodward Ave, Royal Oak, MI 48073, USA., Hazelton D; Comprehensive Urology-A Division of Michigan Healthcare Professionals, 31157 Woodward Ave, Royal Oak, MI 48073, USA., Makhlouf B; Comprehensive Urology-A Division of Michigan Healthcare Professionals, 31157 Woodward Ave, Royal Oak, MI 48073, USA., Wenzler D; Comprehensive Urology-A Division of Michigan Healthcare Professionals, 31157 Woodward Ave, Royal Oak, MI 48073, USA., Sabry M; Comprehensive Urology-A Division of Michigan Healthcare Professionals, 31157 Woodward Ave, Royal Oak, MI 48073, USA., Burks F; Comprehensive Urology-A Division of Michigan Healthcare Professionals, 31157 Woodward Ave, Royal Oak, MI 48073, USA., Penaranda M; Pathnostics, 17661 Cowan, Irvine, CA 92614, USA., Smith DE; Pathnostics, 17661 Cowan, Irvine, CA 92614, USA., Cacdac P; Beaumont Hospital, 3601 W. Thirteen Mile Rd, Royal Oak, MI 48073, USA.; Pathnostics, 17661 Cowan, Irvine, CA 92614, USA.; Department of Microbiology and Immunology, Loyola University Chicago, Maywood, IL, USA.; Comprehensive Urology-A Division of Michigan Healthcare Professionals, 31157 Woodward Ave, Royal Oak, MI 48073, USA.; Regional Urology, LLC, 255 Bert Kouns, Shreveport, LA 71106, USA.; Urologic Consultants of SE PA, 1 Presidential Blvd, Suite 115, Bala Cynwyd, PA 19004, USA.; Minnesota Urology, 11850 Blackfoot Street NW, Suite #470, Coon Rapids, MN 55125, USA.; Premier Urology, 10 Parsonage Road, Suite # 118, Edison, NJ, 08837, USA.; Urology San Antonio, 7909 Fredericksburg Rd, Suite 150, San Antonio, TX, 78229, USA.; Advanced Urology of SO FL, LLC, 5350 W Atlantic Blvd, #102, Delray Beach, FL 33484, USA.; Carolina Urologic Research Center, 823 82nd Parkway, Suite B, Myrtle Beach, SC 29572, USA.; Kelly Statistical Consulting, Palomar Airport Rd, Carlsbad, CA 92011, USA.; University of Kentucky College of Medicine, 800 Rose Street MN 150, Lexington, KY 40506, USA., Sirls L; Comprehensive Urology-A Division of Michigan Healthcare Professionals, 31157 Woodward Ave, Royal Oak, MI 48073, USA. |
| المصدر: | Journal of surgical urology [J Surg Urol] 2020; Vol. 1. Date of Electronic Publication: 2020 May 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Asteroid Publishers Country of Publication: United States NLM ID: 101768126 Publication Model: Print-Electronic Cited Medium: Internet NLM ISO Abbreviation: J Surg Urol Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Lisle, IL : Asteroid Publishers |
| مستخلص: | Introduction: Antimicrobial susceptibility is well characterized in monomicrobial infections, but bacterial species often coexist with other bacterial species. Antimicrobial susceptibility is often tested against single bacterial isolates; this approach ignores interactions between cohabiting bacteria that could impact susceptibility. Here, we use Pooled Antibiotic Susceptibility Testing to compare antimicrobial susceptibility patterns exhibited by polymicrobial and monomicrobial urine specimens obtained from patients with urinary tract infection symptoms. Methods: Urine samples were collected from patients who had symptoms consistent with a urinary tract infection. Multiplex polymerase chain reaction testing was performed to identify and quantify 31 bacterial species. Antibiotic susceptibility was determined using a novel Pooled Antibiotic Susceptibility Testing method. Antibiotic resistance rates in polymicrobial specimens were compared with those in monomicrobial infections. Using a logistic model, resistance rates were estimated when specific bacterial species were present. To assess interactions between pairs of bacteria, the predicted resistance rates were compared when a pair of bacterial species were present versus when just one bacterial species was present. Results: Urine specimens were collected from 3,124 patients with symptoms of urinary tract infection. Of these, multiplex polymerase chain reaction testing detected bacteria in 61.1% (1910) of specimens. Pooled Antibiotic Susceptibility Testing results were available for 70.8% (1352) of these positive specimens. Of these positive specimens, 43.9% (594) were monomicrobial, while 56.1% (758) were polymicrobial. The odds of resistance to ampicillin (p = 0.005), amoxicillin/clavulanate (p = 0.008), five different cephalosporins, vancomycin (p = <0.0001), and tetracycline (p = 0.010) increased with each additional species present in a polymicrobial specimen. In contrast, the odds of resistance to piperacillin/tazobactam decreased by 75% for each additional species present (95% CI 0.61, 0.94, p = 0.010). For one or more antibiotics tested, thirteen pairs of bacterial species exhibited statistically significant interactions compared with the expected resistance rate obtained with the Highest Single Agent Principle and Union Principle. Conclusion: Bacterial interactions in polymicrobial specimens can result in antimicrobial susceptibility patterns that are not detected when bacterial isolates are tested by themselves. Optimizing an effective treatment regimen for patients with polymicrobial infections may depend on accurate identification of the constituent species, as well as results obtained by Pooled Antibiotic Susceptibility Testing. |
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| معلومات مُعتمدة: | R01 DK104718 United States DK NIDDK NIH HHS |
| فهرسة مساهمة: | Keywords: Antibiotic Susceptibility; Bacterial Interactions; Clinical Microbiology; Diagnostic Test; Microbiome; Microbiota; Polymicrobial; Statistical Models; Urinary Tract Infection |
| تواريخ الأحداث: | Date Created: 20221123 Latest Revision: 20221126 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9678350 |
| PMID: | 36416755 |
| قاعدة البيانات: | MEDLINE |
| الوصف غير متاح. |