دورية أكاديمية
أعرض في EDS

Colorimetric detection of Pseudomonas aeruginosa by aptamer-functionalized gold nanoparticles.

التفاصيل البيبلوغرافية
العنوان: Colorimetric detection of Pseudomonas aeruginosa by aptamer-functionalized gold nanoparticles.
المؤلفون: Schmitz FRW; Department of Chemical Engineering and Food Engineering (EQA), Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil., Cesca K; Department of Chemical Engineering and Food Engineering (EQA), Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil. karinacesca@gmail.com., Valério A; Department of Chemical Engineering and Food Engineering (EQA), Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil., de Oliveira D; Department of Chemical Engineering and Food Engineering (EQA), Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil., Hotza D; Department of Chemical Engineering and Food Engineering (EQA), Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.
المصدر: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2023 Jan; Vol. 107 (1), pp. 71-80. Date of Electronic Publication: 2022 Nov 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer International Country of Publication: Germany NLM ID: 8406612 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0614 (Electronic) Linking ISSN: 01757598 NLM ISO Abbreviation: Appl Microbiol Biotechnol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin ; New York : Springer International, c1984-
مواضيع طبية MeSH: Aptamers, Nucleotide* , Metal Nanoparticles*/chemistry , Biosensing Techniques*/methods, Gold/chemistry ; Colorimetry/methods ; Pseudomonas aeruginosa ; Sodium Chloride/chemistry ; Escherichia coli ; Limit of Detection
مستخلص: Novel rapid methodologies for the detection of bacteria have been recently investigated and applied. In hospital environments, infections by pathogens are very common and can cause serious health problems. Pseudomonas aeruginosa is one of the most common bacteria, which can grow in hospital equipment such as catheters and respirators. Even at low concentrations, it can cause severe infections as it is resistant to antibiotics and other treatments. Based on this subject's relevance, this work aimed to develop a colorimetric biosensor using aptamer-functionalized gold nanoparticles for identifying P. aeruginosa. The detection mechanism is based on the color change of gold nanoparticles (AuNPs) from red to blue-purple through NaCl induction after bacteria incubation and aptamer-target binding. First, AuNPs were synthesized and characterized. The influence of aptamer and sodium chloride concentration on the agglomeration of AuNPs was investigated. Optimization of aptamer concentration and salt addition were performed. The best condition for detection was 5 µM aptamers and 200 mM of NaCl. In this case, P. aeruginosa was detected after 5 h for concentrations from 10 8 to 10 5  CFU mL -1 , being 10 5 and 10 4  CFU mL -1 the detection limit for color change by the naked eye and UV-Vis spectrometry, respectively. In addition, other bacteria such as E. coli, S. typhimurium, and Enterobacteriaceae bacterium were also detected with color changing from red to gray. Finally, it was confirmed that the salt incubation time can be 2 h, and that the ideal aptamer concentration is 5 µM. Thus, the colorimetric analysis can be a simple and fast detection method for P. aeruginosa in the range of 10 8 to 10 5  CFU mL -1 to the naked eye. KEY POINTS: • A new method for rapid detection of Pseudomonas aeruginosa • Aptamers conjugated with gold nanoparticles allow pathogen detection by colorimetry • No need for previous surface modification of nanoparticles.
(© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
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فهرسة مساهمة: Keywords: Aptasensor; Bacteria; Biosensor; DNA aptamers; Gold nanoparticles
المشرفين على المادة: 7440-57-5 (Gold)
0 (Aptamers, Nucleotide)
451W47IQ8X (Sodium Chloride)
تواريخ الأحداث: Date Created: 20221123 Date Completed: 20221216 Latest Revision: 20221222
رمز التحديث: 20231215
DOI: 10.1007/s00253-022-12283-5
PMID: 36418544
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-0614
DOI:10.1007/s00253-022-12283-5