Liquid chromatography-tandem mass spectrometry method for the bioanalysis of N,N-dimethyltryptamine (DMT) and its metabolites DMT-N-oxide and indole-3-acetic acid in human plasma.

التفاصيل البيبلوغرافية
العنوان:	Liquid chromatography-tandem mass spectrometry method for the bioanalysis of N,N-dimethyltryptamine (DMT) and its metabolites DMT-N-oxide and indole-3-acetic acid in human plasma.
المؤلفون:	Luethi D; Division of Clinical Pharmacology and Toxicology, Department of Biomedicine and Department of Pharmaceutical Sciences, University Hospital Basel and University of Basel, Basel, Switzerland., Kolaczynska KE; Division of Clinical Pharmacology and Toxicology, Department of Biomedicine and Department of Pharmaceutical Sciences, University Hospital Basel and University of Basel, Basel, Switzerland., Vogt SB; Division of Clinical Pharmacology and Toxicology, Department of Biomedicine and Department of Pharmaceutical Sciences, University Hospital Basel and University of Basel, Basel, Switzerland., Ley L; Division of Clinical Pharmacology and Toxicology, Department of Biomedicine and Department of Pharmaceutical Sciences, University Hospital Basel and University of Basel, Basel, Switzerland., Erne L; Division of Clinical Pharmacology and Toxicology, Department of Biomedicine and Department of Pharmaceutical Sciences, University Hospital Basel and University of Basel, Basel, Switzerland., Liechti ME; Division of Clinical Pharmacology and Toxicology, Department of Biomedicine and Department of Pharmaceutical Sciences, University Hospital Basel and University of Basel, Basel, Switzerland. Electronic address: matthias.liechti@usb.ch., Duthaler U; Division of Clinical Pharmacology and Toxicology, Department of Biomedicine and Department of Pharmaceutical Sciences, University Hospital Basel and University of Basel, Basel, Switzerland. Electronic address: urs.duthaler@unibas.ch.
المصدر:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2022 Dec 15; Vol. 1213, pp. 123534. Date of Electronic Publication: 2022 Nov 11.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101139554 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-376X (Electronic) Linking ISSN: 15700232 NLM ISO Abbreviation: J Chromatogr B Analyt Technol Biomed Life Sci Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Amsterdam ; New York : Elsevier, c2002-
مواضيع طبية MeSH:	N,N-Dimethyltryptamine* , Oxides*, Humans ; Chromatography, Liquid ; Methanol ; Tandem Mass Spectrometry
مستخلص:	The indole alkaloid N,N-dimethyltryptamine (DMT) induces psychedelic effects in humans. In addition to ceremonial and recreational use, DMT is subject to clinical investigations. Sensitive bioanalytical methods are required to assess the pharmacokinetics of DMT and its metabolites in human plasma. Here, a high performance liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of DMT and its major metabolites indole-3-acetic acid (IAA) and DMT-N-oxide (DMT-NO) was developed and validated. As IAA is an endogenous component of human plasma, ¹³ C 6 -IAA was used to determine IAA concentrations. After simple protein precipitation with methanol, analytes were separated on a pentafluorophenyl column. A gradient consisting of 0.1% (v/v) formic acid in a methanol-water mixture was applied for analyte separation. The analytes were detected by positive electrospray ionization followed by multiple reaction monitoring. The calibration range of the assay was 0.25-250 ng/mL for DMT, 0.1-100 ng/mL for DMT-NO, and 25-25,000 ng/mL for ¹³ C 6 -IAA. The intra- and inter-assay accuracy was 93-113% for all analytes at all quality control levels, with coefficient of variation ≤ 11%. All analytes were stable under storage conditions relevant for the analysis of large batches of study samples. The validated method was capable of assessing pharmacokinetic (PK) parameters of DMT and its metabolites in study participants intravenously perfused with 1 mg/min DMT for 90 min. Overall, the developed method is easy-to-use, has a short run time, and qualifies for PK and metabolism studies of DMT in clinical settings. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
فهرسة مساهمة:	Keywords: Ayahuasca; Bioanalysis; HPLC; Metabolism; Pharmacokinetics; Psychedelic
المشرفين على المادة:	WUB601BHAA (N,N-Dimethyltryptamine) 6U1S09C61L (indoleacetic acid) 0 (Oxides) Y4S76JWI15 (Methanol)
تواريخ الأحداث:	Date Created: 20221126 Date Completed: 20221206 Latest Revision: 20221206
رمز التحديث:	20231215
DOI:	10.1016/j.jchromb.2022.123534
PMID:	36434963
قاعدة البيانات:	MEDLINE

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الوصف
تدمد:	1873-376X
DOI:	10.1016/j.jchromb.2022.123534