دورية أكاديمية
Genetics tools for corpora allata specific gene expression in Aedes aegypti mosquitoes.
| العنوان: | Genetics tools for corpora allata specific gene expression in Aedes aegypti mosquitoes. |
|---|---|
| المؤلفون: | Nouzova M; Department of Biological Sciences and Biomolecular Science Institute, Florida International University, Miami, FL, 33199, USA. marcela.nouzova@paru.cas.cz.; Institute of Parasitology, Biology Center of the Academy of Sciences of the Czech Republic, 37005, České Budějovice, Czech Republic. marcela.nouzova@paru.cas.cz., Edwards MJ; Department of Biology, Muhlenberg College, Allentown, PA, 18104, USA., DeGennaro M; Department of Biological Sciences and Biomolecular Science Institute, Florida International University, Miami, FL, 33199, USA., Leyva D; Department of Chemistry and Biochemistry and Biomolecular Science Institute, Florida International University, Miami, FL, 33199, USA., Tose LV; Department of Chemistry and Biochemistry and Biomolecular Science Institute, Florida International University, Miami, FL, 33199, USA., Fernandez-Lima F; Department of Chemistry and Biochemistry and Biomolecular Science Institute, Florida International University, Miami, FL, 33199, USA., Noriega FG; Department of Biological Sciences and Biomolecular Science Institute, Florida International University, Miami, FL, 33199, USA. noriegaf@fiu.edu.; Department of Parasitology, University of South Bohemia, České Budějovice, Czech Republic. noriegaf@fiu.edu. |
| المصدر: | Scientific reports [Sci Rep] 2022 Nov 28; Vol. 12 (1), pp. 20426. Date of Electronic Publication: 2022 Nov 28. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Corpora Allata* , Aedes*/genetics, Animals ; Juvenile Hormones ; Animals, Genetically Modified ; Drosophila ; Green Fluorescent Proteins/genetics ; Gene Expression |
| مستخلص: | Juvenile hormone (JH) is synthesized by the corpora allata (CA) and controls development and reproduction in insects. Therefore, achieving tissue-specific expression of transgenes in the CA would be beneficial for mosquito research and control. Different CA promoters have been used to drive transgene expression in Drosophila, but mosquito CA-specific promoters have not been identified. Using the CRISPR/Cas9 system, we integrated transgenes encoding the reporter green fluorescent protein (GFP) close to the transcription start site of juvenile hormone acid methyl transferase (JHAMT), a locus encoding a JH biosynthetic enzyme, specifically and highly expressed in the CA of Aedes aegypti mosquitoes. Transgenic individuals showed specific GFP expression in the CA but failed to reproduce the full pattern of jhamt spatiotemporal expression. In addition, we created GeneSwitch driver and responder mosquito lines expressing an inducible fluorescent marker, enabling the temporal regulation of the transgene via the presence or absence of an inducer drug. The use of the GeneSwitch system has not previously been reported in mosquitoes and provides a new inducible binary system that can control transgene expression in Aedes aegypti. (© 2022. The Author(s).) |
| References: | Curr Biol. 2005 Oct 25;15(20):1796-807. (PMID: 16182527) Insect Biochem Mol Biol. 2014 Aug;51:1-9. (PMID: 24833260) Int Sch Res Notices. 2014 Oct 19;2014:967361. (PMID: 27382622) Nat Rev Genet. 2016 Jul;17(7):407-421. (PMID: 27240813) Cell. 2010 Apr 30;141(3):536-48. (PMID: 20434990) J Genet Genomics. 2019 Apr 20;46(4):213-220. (PMID: 31060819) G3 (Bethesda). 2012 Nov;2(11):1305-15. (PMID: 23173082) Insect Biochem Mol Biol. 2020 Dec;127:103490. (PMID: 33169702) Methods Mol Biol. 2016;1478:53-78. (PMID: 27730575) Science. 2007 Jun 22;316(5832):1718-23. (PMID: 17510324) Insect Mol Biol. 2007 Feb;16(1):61-71. (PMID: 17257209) Genetics. 2010 Oct;186(2):735-55. (PMID: 20697123) PLoS One. 2012;7(2):e31552. (PMID: 22348104) Proc Natl Acad Sci U S A. 2000 Sep 26;97(20):10895-8. (PMID: 11005862) BMC Bioinformatics. 2006 Dec 18;7 Suppl 5:S15. (PMID: 17254299) Insect Biochem Mol Biol. 2009 Jan;39(1):31-7. (PMID: 18984053) Genetics. 2012 Mar;190(3):1139-44. (PMID: 22209908) J Comp Neurol. 2017 May 1;525(7):1743-1755. (PMID: 28152579) Insect Mol Biol. 2006 Aug;15(4):403-10. (PMID: 16907827) Dev Genet. 1994;15(2):188-200. (PMID: 8205725) Genesis. 2007 Apr;45(4):208-17. (PMID: 17417802) Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10775-80. (PMID: 11517299) Insect Biochem Mol Biol. 2006 Sep;36(9):694-700. (PMID: 16935218) Trends Parasitol. 2022 Jan;38(1):54-66. (PMID: 34483052) Mol Gen Genet. 1997 Apr 16;254(3):291-6. (PMID: 9150263) Genetics. 2004 Aug;167(4):1781-90. (PMID: 15342516) Nat Commun. 2016 Oct 03;7:13010. (PMID: 27694947) Proc Natl Acad Sci U S A. 2021 Nov 9;118(45):. (PMID: 34697248) PLoS One. 2016 Aug 29;11(8):e0161817. (PMID: 27570965) PLoS Genet. 2015 Mar 16;11(3):e1005038. (PMID: 25774983) Nature. 2018 Nov;563(7732):501-507. (PMID: 30429615) Insect Biochem Mol Biol. 2020 Jan;116:103287. (PMID: 31760138) Insect Biochem Mol Biol. 2003 Dec;33(12):1307-15. (PMID: 14599502) Curr Opin Insect Sci. 2018 Dec;30:8-18. (PMID: 30553490) Insect Biochem Mol Biol. 2011 Sep;41(9):660-9. (PMID: 21554954) Insect Mol Biol. 2002 Feb;11(1):87-95. (PMID: 11841506) Development. 1993 Jun;118(2):401-15. (PMID: 8223268) Nat Methods. 2011 Dec 28;9(1):47-55. (PMID: 22205518) Insect Biochem Mol Biol. 2011 Dec;41(12):938-45. (PMID: 21968404) Nat Commun. 2021 Jul 19;12(1):4388. (PMID: 34282149) Nat Rev Genet. 2005 Mar;6(3):167-78. (PMID: 15738961) Proc Natl Acad Sci U S A. 2001 Oct 23;98(22):12596-601. (PMID: 11675495) Elife. 2019 May 21;8:. (PMID: 31112133) Insect Biochem Mol Biol. 2011 Aug;41(8):637-44. (PMID: 21536128) Cell Rep. 2015 Mar 17;10(10):1681-1691. (PMID: 25772356) |
| معلومات مُعتمدة: | 22-21244S Grantová Agentura České Republiky; R21 AI167849 United States AI NIAID NIH HHS; R21 AI153689 United States AI NIAID NIH HHS |
| المشرفين على المادة: | 0 (Juvenile Hormones) 147336-22-9 (Green Fluorescent Proteins) |
| تواريخ الأحداث: | Date Created: 20221128 Date Completed: 20221130 Latest Revision: 20230113 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC9705396 |
| DOI: | 10.1038/s41598-022-25009-4 |
| PMID: | 36443489 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 2045-2322 |
|---|---|
| DOI: | 10.1038/s41598-022-25009-4 |