دورية أكاديمية
Electrophoretic mobilization in capillary isoelectric focusing by a weak acid or an acidic ampholyte as catholyte assessed by computer simulation.
العنوان: | Electrophoretic mobilization in capillary isoelectric focusing by a weak acid or an acidic ampholyte as catholyte assessed by computer simulation. |
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المؤلفون: | Thormann W; Institute for Infectious Diseases, University of Bern, Bern, Switzerland. |
المصدر: | Electrophoresis [Electrophoresis] 2023 Apr; Vol. 44 (7-8), pp. 656-666. Date of Electronic Publication: 2022 Dec 14. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 8204476 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1522-2683 (Electronic) Linking ISSN: 01730835 NLM ISO Abbreviation: Electrophoresis Subsets: MEDLINE |
أسماء مطبوعة: | Publication: Original Publication: [Weinheim, Germany] : Verlag Chemie, [1980- |
مواضيع طبية MeSH: | Ampholyte Mixtures*/chemistry , Capillary Isoelectric Focusing*, Computer Simulation ; Isoelectric Focusing/methods ; Aspartic Acid ; Glutamic Acid ; Acetates ; Hydrogen-Ion Concentration |
مستخلص: | Capillary isoelectric focusing (CIEF) with cationic electrophoretic mobilization induced via replacing the catholyte with the anolyte or a solution of another acid or amino acid was investigated by computer simulation for a wide range pH gradient bracketed between two amphoteric spacers and short electrode vials with a higher id than the capillary. Dynamic simulations provide insight into the complexity of the mobilizing process in a hitherto inaccessible way. The electrophoretic mobilizing process begins with the penetration of the mobilizing compound through the entire capillary, is followed by a gradual or steplike decrease of pH, and ends in an environment with a non-homogenous solution of the mobilizer. Analytes do not necessarily pass the point of detection in the order of decreasing pI values. Cationic mobilization encompasses an inherent zone dispersing and refocusing process toward the capillary end. This behavior is rather strong with phosphoric acid and citric acid, moderate with aspartic acid, glutamic acid (GLU), formic acid, and acetic acid and less pronounced in the absence of the cathodic spacer. The data reveal that optical detectors should not be placed before 90% of capillary length. Aspartic acid, GLU, formic acid, and acetic acid provide an environment with a continuously decreasing pH that explains their successful use in optimized two-step CIEF protocols. (© 2022 Wiley-VCH GmbH.) |
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فهرسة مساهمة: | Keywords: capillary isoelectric focusing; chemical mobilization; computer simulation; electrophoretic mobilization |
المشرفين على المادة: | 0 (Ampholyte Mixtures) 0YIW783RG1 (formic acid) 30KYC7MIAI (Aspartic Acid) 3KX376GY7L (Glutamic Acid) 0 (Acetates) |
تواريخ الأحداث: | Date Created: 20221130 Date Completed: 20230419 Latest Revision: 20230420 |
رمز التحديث: | 20230421 |
DOI: | 10.1002/elps.202200262 |
PMID: | 36448503 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1522-2683 |
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DOI: | 10.1002/elps.202200262 |