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Increasing buffering capacity enhances rumen fermentation characteristics and alters rumen microbiota composition of high-concentrate fed Hanwoo steers.

التفاصيل البيبلوغرافية
العنوان: Increasing buffering capacity enhances rumen fermentation characteristics and alters rumen microbiota composition of high-concentrate fed Hanwoo steers.
المؤلفون: Ramos SC; Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon, 57922, Republic of Korea., Kim SH; Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon, 57922, Republic of Korea., Jeong CD; Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon, 57922, Republic of Korea., Mamuad LL; Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon, 57922, Republic of Korea., Son AR; Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon, 57922, Republic of Korea., Kang SH; Faculty of Medicine, The University of Queensland Diamantina Institute, Brisbane, Australia., Cho YI; Animal Disease and Diagnostic Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon, 57922, Republic of Korea., Kim TG; Rupromin Balance™, 5th. Bonsol Blg. 445, Teheran-ro, Gangnam-gu, Seoul, 06158, Republic of Korea., Lee JS; Rupromin Balance™, 5th. Bonsol Blg. 445, Teheran-ro, Gangnam-gu, Seoul, 06158, Republic of Korea., Cho KK; Department of Animal Resources Technology, Gyeongnam National University of Science and Technology, Jinju, 52725, Republic of Korea., Lee SS; Institute of Agriculture and Life Science and University-Centered Labs, Gyeongsang National University, Jinju, 52828, Republic of Korea., Lee SS; Ruminant Nutrition and Anaerobe Laboratory, Department of Animal Science and Technology, Sunchon National University, 413 Jungangno, Jeonnam, Suncheon, 57922, Republic of Korea. rumen@scnu.ac.kr.
المصدر: Scientific reports [Sci Rep] 2022 Dec 01; Vol. 12 (1), pp. 20739. Date of Electronic Publication: 2022 Dec 01.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Rumen* , Microbiota*, Humans ; Animals ; Fermentation ; Proteobacteria ; Firmicutes
مستخلص: The buffering capacity of buffer agents and their effects on in vitro and in vivo rumen fermentation characteristics, and bacterial composition of a high-concentrate fed Hanwoo steers were investigated in this study. Treatments were comprised of CON (no buffer added), BC 0.3% (low buffering capacity, 0.3% buffer), BC 0.5% (medium buffering capacity, 0.5% buffer), and BC 0.9% (high buffering capacity, 0.9% buffer). Four Hanwoo steers in a 4 × 4 Latin square design were used for the in vivo trial to assess the effect of treatments. Results on in vitro experiment showed that buffering capacity, pH, and ammonia-nitrogen concentration (NH 3 -N) were significantly higher in BC 0.9% and BC 0.5% than the other treatments after 24 h incubation. Individual and total volatile fatty acids (VFA) concentration of CON were lowest compared to treatment groups. Meanwhile, in vivo experiment revealed that Bacteroidetes were dominant for all treatments followed by Firmicutes and Proteobacteria. The abundances of Barnesiella intestinihominis, Treponema porcinum, and Vibrio marisflavi were relatively highest under BC 0.9%, Ruminoccocus bromii and Succiniclasticum ruminis under BC 0.5% , and Bacteroides massiliensis under BC 0.3%. The normalized data of relative abundance of observed OTUs' representative families have grouped the CON with BC 0.3% in the same cluster, whereas BC 0.5% and BC 0.9% were clustered separately which indicates the effect of varying buffering capacity of buffer agents. Principal coordinate analysis (PCoA) on unweighted UniFrac distances revealed close similarity of bacterial community structures within and between treatments and control, in which BC 0.9% and BC 0.3% groups showed dispersed community distribution. Overall, increasing the buffering capacity by supplementation of BC 0.5% and and BC 0.9% buffer agents enhanced rumen fermentation characteristics and altered the rumen bacterial community, which could help prevent ruminal acidosis during a high-concentrate diet.
(© 2022. The Author(s).)
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تواريخ الأحداث: Date Created: 20221201 Date Completed: 20221205 Latest Revision: 20230111
رمز التحديث: 20230111
مُعرف محوري في PubMed: PMC9715728
DOI: 10.1038/s41598-022-24777-3
PMID: 36456638
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-022-24777-3