دورية أكاديمية
Host-delivered RNAi-mediated silencing using fusion cassettes of different functional groups of genes precludes Meloidogyne incognita multiplication in Nicotiana tabacum.
| العنوان: | Host-delivered RNAi-mediated silencing using fusion cassettes of different functional groups of genes precludes Meloidogyne incognita multiplication in Nicotiana tabacum. |
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| المؤلفون: | Hada A; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Singh D; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Banakar P; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India. prakashbanakar@hau.ac.in.; Department of Nematology and Centre for Bio-Nanotechnology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004, India. prakashbanakar@hau.ac.in., Papolu PK; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Kassam R; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Chatterjee M; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Yadav J; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India., Rao U; Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012, India. umarao@iari.res.in. |
| المصدر: | Plant cell reports [Plant Cell Rep] 2023 Jan; Vol. 42 (1), pp. 29-43. Date of Electronic Publication: 2022 Dec 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer Country of Publication: Germany NLM ID: 9880970 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-203X (Electronic) Linking ISSN: 07217714 NLM ISO Abbreviation: Plant Cell Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Berlin ; New York : Springer, 1981- |
| مواضيع طبية MeSH: | Nicotiana*/genetics , Tylenchoidea*/genetics, Animals ; Female ; RNA Interference ; Gene Silencing ; Plants, Genetically Modified/genetics ; RNA, Double-Stranded/genetics ; Plant Diseases/genetics |
| مستخلص: | Key Message: This study demonstrates multi-gene silencing approach for simultaneous silencing of several functional genes through a fusion gene strategy for protecting plants against root-knot nematode, Meloidogyne incognita. The ability of root-knot nematode (RKN), Meloidogyne incognita, to cause extensive yield decline in a wide range of cultivated crops is well-documented. Due to the inadequacies of current management approaches, the alternatively employed contemporary RNA interference (RNAi)-based host-delivered gene silencing (HD-RNAi) strategy targeting different functional effectors/genes has shown substantial potential to combat RKNs. In this direction, we have explored the possibility of simultaneous silencing of four esophageal gland genes, six plant cell-wall modifying enzymes (PCWMEs) and a serine protease gene of M. incognita using the fusion approach. In vitro RNAi showed that combinatorial gene silencing is the most effective in affecting nematode behavior in terms of reduced attraction, penetration, development, and reproduction in tomato and adzuki beans. In addition, qRT-PCR analysis of M. incognita J2s soaked in fusion-dsRNA showed perturbed expression of all the genes comprising the fusion construct confirming successful dsRNA processing which is also supported by increased mRNA abundance of five key-RNAi pathway genes. In addition, hairpin RNA expressing constructs of multi-gene fusion cassettes were developed and used for generation of Nicotiana tabacum transgenic plants. The integration of gene constructs and expression of siRNAs in transgenic events were confirmed by Southern and Northern blot analyses. Besides, bio-efficacy analyses of transgenic events, conferred up to 87% reduction in M. incognita multiplication. Correspondingly, reduced transcript accumulation of the target genes in the M. incognita females extracted from transgenic events confirmed successful gene silencing. (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| معلومات مُعتمدة: | BT/PR5908/AGR/36/727/2012 Department of Biotechnology, Ministry of Science and Technology, India; BT/PR-18924/COE/34/48/2017 Department of Biotechnology, Ministry of Science and Technology, India |
| فهرسة مساهمة: | Keywords: Combinatorial RNA-interference; Effectors; Gene silencing; HD-RNAi; Meloidogyne incognita; Plant–parasitic nematodes |
| المشرفين على المادة: | 0 (RNA, Double-Stranded) |
| تواريخ الأحداث: | Date Created: 20221203 Date Completed: 20230124 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s00299-022-02934-2 |
| PMID: | 36462028 |
| قاعدة البيانات: | MEDLINE |
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| DOI: | 10.1007/s00299-022-02934-2 |