دورية أكاديمية
Rapid and low-cost liquid biopsy with ATR-FTIR spectroscopy to discriminate the molecular subtypes of breast cancer.
| العنوان: | Rapid and low-cost liquid biopsy with ATR-FTIR spectroscopy to discriminate the molecular subtypes of breast cancer. |
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| المؤلفون: | Pereira de Souza NM; Department of Life Sciences, University of Santa Cruz do Sul, Santa Cruz do Sul, RS Brazil. Electronic address: nikolas1@mx2.unisc.br., Machado BH; International affairs, International University Center, Santa Cruz do Sul, RS, Brazil. Electronic address: brendamachado664@gmail.com., Padoin LV; Mastology Service at the Hospital of the Federal University of Santa Maria, Santa Maria, RS, Brazil. Electronic address: drpadoin@gmail.com., Prá D; Department of Life Sciences, University of Santa Cruz do Sul, Santa Cruz do Sul, RS Brazil; Postgraduate Program in Health Promotion, University of Santa Cruz do Sul, Santa Cruz do Sul, RS, Brazil. Electronic address: daniel_pra@yahoo.com., Fay AP; Postgraduate Program in Medicine and Health Sciences, School of Medicine, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: andre.fay@pucrs.br., Corbellini VA; Postgraduate Program in Health Promotion, University of Santa Cruz do Sul, Santa Cruz do Sul, RS, Brazil; Department of Sciences, Humanities and Education, University of Santa Cruz do Sul, Santa Cruz do Sul, RS, Brazil; Postgraduate Program in Environmental Technology, University of Santa Cruz do Sul, RS, Brazil. Electronic address: valer@unisc.br., Rieger A; Department of Life Sciences, University of Santa Cruz do Sul, Santa Cruz do Sul, RS Brazil; Postgraduate Program in Health Promotion, University of Santa Cruz do Sul, Santa Cruz do Sul, RS, Brazil; Postgraduate Program in Environmental Technology, University of Santa Cruz do Sul, RS, Brazil. Electronic address: rieger@unisc.br. |
| المصدر: | Talanta [Talanta] 2023 Mar 01; Vol. 254, pp. 123858. Date of Electronic Publication: 2022 Aug 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 2984816R Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3573 (Electronic) Linking ISSN: 00399140 NLM ISO Abbreviation: Talanta Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Oxford : Pergamon Press |
| مواضيع طبية MeSH: | Breast Neoplasms*/diagnosis, Female ; Humans ; Discriminant Analysis ; Least-Squares Analysis ; Multivariate Analysis ; Spectroscopy, Fourier Transform Infrared/methods |
| مستخلص: | Breast cancer (BC) is the most prevalent cancer worldwide. The prognosis and survival of these patients are directly related to the diagnostic stage. Even so, the gold standard screening method (mammography) has a long waiting period, high rates of false positives, anxiety for patients, and consequently delays the diagnosis by core needle biopsy (invasive method). Alternatively, the Attenuated Total Reflection Fourier Transform Infrared (ATR-FTIR) spectroscopy is a noninvasive, low-cost, rapid, and reagent-free technique that generates the spectral metabolomic profile of biomolecules. This makes it possible to assess systemic repercussions, such as the BC carcinogenesis process. Blood plasma samples (n = 56 BC and n = 18 controls) were analyzed in the spectrophotometer in the ATR-FTIR mode. For the exploratory analysis of the data, interval Principal Component Analysis (iPCA) was used, and for predictive chemometric modeling, the Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) algorithm with validation by leave-one-out cross-validation. iPCA in the region of 1118-1052 cm -1 (predominantly DNA/RNA bands) showed significant clustering of molecular subtypes and control. The OPLS-DA model achieved 100% accuracy with only 1 latent variable and Root Mean Square Error of Cross-Validation (RMSECV) < 0.005 for all molecular subtypes and control. The wavenumbers (cm -1 ) with the highest iPCA peaks (loadings: 1117, 1089, 1081, 1075, 1057, and 1052) were used as input to MANOVA (Wilks' Lambda, p < 0.001 between molecular subtypes and control). The rapid and low-cost detection of BC molecular subtypes by ATR-FTIR spectroscopy would plausibly allow initial screening and clinical management, improving prognosis, reducing mortality and costs for the health system. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Breast cancer; Chemometrics; Infrared spectroscopy; Liquid biopsy; Orthogonal partial least squares discriminant analysis |
| المشرفين على المادة: | I03LI0901Y (isopropyl cyanoacrylate) |
| تواريخ الأحداث: | Date Created: 20221205 Date Completed: 20230106 Latest Revision: 20230106 |
| رمز التحديث: | 20230106 |
| DOI: | 10.1016/j.talanta.2022.123858 |
| PMID: | 36470017 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3573 |
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| DOI: | 10.1016/j.talanta.2022.123858 |