دورية أكاديمية
A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates.
| العنوان: | A human fetal lung cell atlas uncovers proximal-distal gradients of differentiation and key regulators of epithelial fates. |
|---|---|
| المؤلفون: | He P; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Cambridge, UK., Lim K; Wellcome Trust/CRUK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK., Sun D; Wellcome Trust/CRUK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK., Pett JP; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK., Jeng Q; Wellcome Trust/CRUK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK., Polanski K; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK., Dong Z; Wellcome Trust/CRUK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK., Bolt L; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK., Richardson L; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK., Mamanova L; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK., Dabrowska M; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK., Wilbrey-Clark A; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK., Madissoon E; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Cambridge, UK., Tuong ZK; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; Molecular Immunity Unit, University of Cambridge Department of Medicine, Cambridge, UK., Dann E; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK., Suo C; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; Department of Paediatrics, Cambridge University Hospitals, Hills Road, Cambridge CB2 0 QQ, UK., Goh I; Biosciences Institute, Newcastle University, Newcastle upon Tyne, NE2 4HH, UK., Yoshida M; Lungs for Living Research Centre, UCL Respiratory, University College London, London, UK., Nikolić MZ; Lungs for Living Research Centre, UCL Respiratory, University College London, London, UK., Janes SM; Lungs for Living Research Centre, UCL Respiratory, University College London, London, UK., He X; John van Geest Centre for Brain Repair, Department of Clinical Neurosciences and Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK., Barker RA; John van Geest Centre for Brain Repair, Department of Clinical Neurosciences and Wellcome-MRC Cambridge Stem Cell Institute, University of Cambridge, Cambridge, UK., Teichmann SA; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; Department of Physics, Cavendish Laboratory, University of Cambridge, Cambridge CB3 0HE, UK., Marioni JC; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK; European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Wellcome Genome Campus, Cambridge, UK; Cancer Research UK Cambridge Institute, University of Cambridge, Cambridge, UK., Meyer KB; Wellcome Sanger Institute, Hinxton, Cambridge CB10 1SA, UK., Rawlins EL; Wellcome Trust/CRUK Gurdon Institute, Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge CB2 1QN, UK. Electronic address: e.rawlins@gurdon.cam.ac.uk. |
| المصدر: | Cell [Cell] 2022 Dec 08; Vol. 185 (25), pp. 4841-4860.e25. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 0413066 Publication Model: Print Cited Medium: Internet ISSN: 1097-4172 (Electronic) Linking ISSN: 00928674 NLM ISO Abbreviation: Cell Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge, Ma : Cell Press Original Publication: Cambridge, MIT Press. |
| مواضيع طبية MeSH: | Lung*/cytology , Fetus*/cytology, Humans ; Cell Differentiation ; Gene Expression Profiling ; Organogenesis ; Organoids ; Atlases as Topic |
| مستخلص: | We present a multiomic cell atlas of human lung development that combines single-cell RNA and ATAC sequencing, high-throughput spatial transcriptomics, and single-cell imaging. Coupling single-cell methods with spatial analysis has allowed a comprehensive cellular survey of the epithelial, mesenchymal, endothelial, and erythrocyte/leukocyte compartments from 5-22 post-conception weeks. We identify previously uncharacterized cell states in all compartments. These include developmental-specific secretory progenitors and a subtype of neuroendocrine cell related to human small cell lung cancer. Our datasets are available through our web interface (https://lungcellatlas.org). To illustrate its general utility, we use our cell atlas to generate predictions about cell-cell signaling and transcription factor hierarchies which we rigorously test using organoid models. Competing Interests: Declaration of interests S.A.T. is a member of the Scientific Advisory Board for the following companies: Biogen, Foresite Labs, GSK, Qiagen, CRG Barcelona, Jax Labs, SciLife Lab, and Allen Institute. She is a consultant for Genentech and Roche. She is co-founder of Transition Bio and a member of the Board. Z.K.T. has received consulting fees from Synteny Biotechnologies for activities unrelated to this work. (Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| التعليقات: | Comment in: Cell Stem Cell. 2023 Jan 5;30(1):5-6. (PMID: 36608678) |
| معلومات مُعتمدة: | MC_PC_17230 United Kingdom MRC_ Medical Research Council; MR/S035907/1 United Kingdom MRC_ Medical Research Council; MR/S036334/1 United Kingdom MRC_ Medical Research Council; G108/596 United Kingdom MRC_ Medical Research Council; MR/R006237/1 United Kingdom MRC_ Medical Research Council; MR/P009581/1 United Kingdom MRC_ Medical Research Council; MR/S005579/1 United Kingdom MRC_ Medical Research Council; MR/W00111X/1 United Kingdom MRC_ Medical Research Council; MR/R015635/1 United Kingdom MRC_ Medical Research Council |
| فهرسة مساهمة: | Keywords: differentiation; epigenetics; fibroblast; genomics; multiomic; organoids; perturbation; single-cell; spatial; transcription factor |
| تواريخ الأحداث: | Date Created: 20221209 Date Completed: 20221219 Latest Revision: 20240313 |
| رمز التحديث: | 20240313 |
| DOI: | 10.1016/j.cell.2022.11.005 |
| PMID: | 36493756 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1097-4172 |
|---|---|
| DOI: | 10.1016/j.cell.2022.11.005 |