دورية أكاديمية
Comprehensive proteomic profiling of early antral follicles from sheep.
| العنوان: | Comprehensive proteomic profiling of early antral follicles from sheep. |
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| المؤلفون: | Otávio KS; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil., Passos JRS; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil., Silva RF; School of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Lima LF; School of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Cadenas J; School of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Paes VM; School of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Correia HHV; School of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Ferreira ACA; School of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Canafístula FG; School of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Bezerra MJB; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil., Oliveira LLB; Drug Research and Development Center, School of Medicine, Federal University of Ceará, Fortaleza, Brazil., Carvalho GGC; Drug Research and Development Center, School of Medicine, Federal University of Ceará, Fortaleza, Brazil., Paier CRK; Drug Research and Development Center, School of Medicine, Federal University of Ceará, Fortaleza, Brazil., Pessoa C; Drug Research and Development Center, School of Medicine, Federal University of Ceará, Fortaleza, Brazil., Guerreiro DG; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil., Moraes MEA; Drug Research and Development Center, School of Medicine, Federal University of Ceará, Fortaleza, Brazil., Figueiredo JR; School of Veterinary Medicine, State University of Ceará, Fortaleza, Brazil., Moura AA; Department of Animal Science, Federal University of Ceará, Fortaleza, Brazil; Drug Research and Development Center, School of Medicine, Federal University of Ceará, Fortaleza, Brazil. Electronic address: arlindo.moura@gmail.com. |
| المصدر: | Animal reproduction science [Anim Reprod Sci] 2023 Jan; Vol. 248, pp. 107153. Date of Electronic Publication: 2022 Nov 21. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Scientific Pub. Co Country of Publication: Netherlands NLM ID: 7807205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2232 (Electronic) Linking ISSN: 03784320 NLM ISO Abbreviation: Anim Reprod Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam, Elsevier Scientific Pub. Co. |
| مواضيع طبية MeSH: | Proteome* , MicroRNAs*, Animals ; Sheep ; Female ; Chromatography, Liquid/veterinary ; Proteomics ; Tandem Mass Spectrometry/veterinary |
| مستخلص: | The present study evaluates the proteome of early antral follicles from Ovis aries. Fifty follicles were collected from ovaries of adult ewes and extracted proteins were trypsin-digested, desalted and analyzed by LC-MS/MS. Genes were screened for potential modulation by miRNAs and protein data, subjected to functional enrichment analysis. Label-free mass spectrometry allowed the identification of 2503 follicle proteins, confirming vimentin, actin, lamin, heat shock proteins and histones as the most abundant ones. In silico analyses indicated that miRNAs modulate the expression of genes coding proteins of the sheep follicles involved in cell cycle, cell differentiation, aging, apoptosis, cell death, adipocyte differentiation, cell division. The most important biological processes associated with the follicle proteins were innate immune response, translation, adaptive immune response and protein folding, while molecular functions linked to the proteome of sheep antral follicles related to metal ion binding, ATP binding, oxygen binding, RNA binding and GTP binding, among others. Upload of 2503 Uniport accession codes through DAVID platform matched 1274 genes, associated with translation, metabolic process, proteolysis involved in cellular protein catabolic process, zona pellucida receptor complex and others. KEEG pathways analysis indicated genes correlated with ovine follicular development, with major pathways listed as carbon metabolism, biosynthesis of amino acids, glutathione metabolism, oxidative phosphorylation, fatty acid degradation and oocyte meiosis. This represents a comprehensive atlas of proteins expressed in sheep early antral follicles and will contribute to future identification of biomarkers for follicular development and oocyte maturation. Competing Interests: Conflict of interest The authors declare no conflicts of interest. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Antral follicles; Ewes; Mass spectrometry; Proteome |
| المشرفين على المادة: | 0 (Proteome) 0 (MicroRNAs) |
| تواريخ الأحداث: | Date Created: 20221212 Date Completed: 20230111 Latest Revision: 20230111 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1016/j.anireprosci.2022.107153 |
| PMID: | 36502761 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-2232 |
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| DOI: | 10.1016/j.anireprosci.2022.107153 |