دورية أكاديمية
أعرض في EDS

Calcium-dependent subquantal peptide release from single docked lawn-resident vesicles of pituitary lactotrophs.

التفاصيل البيبلوغرافية
العنوان: Calcium-dependent subquantal peptide release from single docked lawn-resident vesicles of pituitary lactotrophs.
المؤلفون: Gonçalves PP; CESAM - Centre for Environmental and Marine Studies, Department of Biology, University of Aveiro, Campus Universitário de Santiago, 3810-193, Aveiro, Portugal., Stenovec M; Laboratory of Neuroendocrinology-Molecular Cell Physiology, Institute of Pathophysiology, University of Ljubljana, Faculty of Medicine, Zaloška 4, 1000, Ljubljana, Slovenia; Laboratory of Cell Engineering, Celica Biomedical, Tehnološki Park 24, 1000, Ljubljana, Slovenia., Grácio L; CRACS & INESC-TEC - Centre for Research in Advanced Computing Systems & Institute for Systems and Computer Engineering, Technology and Science, Department of Computer Science, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007 Porto, Portugal., Kreft M; Laboratory of Neuroendocrinology-Molecular Cell Physiology, Institute of Pathophysiology, University of Ljubljana, Faculty of Medicine, Zaloška 4, 1000, Ljubljana, Slovenia; Laboratory of Cell Engineering, Celica Biomedical, Tehnološki Park 24, 1000, Ljubljana, Slovenia; Department of Biology, University of Ljubljana, Biotechnical Faculty, 1000 Ljubljana, Slovenia., Zorec R; Laboratory of Neuroendocrinology-Molecular Cell Physiology, Institute of Pathophysiology, University of Ljubljana, Faculty of Medicine, Zaloška 4, 1000, Ljubljana, Slovenia; Laboratory of Cell Engineering, Celica Biomedical, Tehnološki Park 24, 1000, Ljubljana, Slovenia. Electronic address: robert.zorec@mf.uni-lj.si.
المصدر: Cell calcium [Cell Calcium] 2023 Jan; Vol. 109, pp. 102687. Date of Electronic Publication: 2022 Dec 10.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8006226 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-1991 (Electronic) Linking ISSN: 01434160 NLM ISO Abbreviation: Cell Calcium Subsets: MEDLINE
أسماء مطبوعة: Publication: <2003->: Amsterdam : Elsevier
Original Publication: [Edinburgh, New York] Churchill Livingston
مواضيع طبية MeSH: Lactotrophs*/metabolism, Rats ; Animals ; Calcium/metabolism ; Prolactin/metabolism ; Rats, Wistar ; Membrane Fusion/physiology ; Peptides/metabolism ; Secretory Vesicles/metabolism ; Exocytosis/physiology
مستخلص: Regulated exocytosis consists of the fusion between vesicles and the plasma membranes, leading to the formation of a narrow fusion pore through which secretions exit the vesicle lumen into the extracellular space. An increase in the cytosolic concentration of free Ca 2+ ([Ca 2+ ] i ) is considered the stimulus of this process. However, whether this mechanism can be preserved in a simplified system of membrane lawns with docked secretory vesicles, devoid of cellular components, is poorly understood. Here, we studied peptide discharge from individual secretory vesicles docked at the plasma membrane, prepared from primary endocrine pituitary cells (the lactotrophs), releasing hormone prolactin. To label secretory vesicles, we transfected lactotrophs to express the fluorescent atrial natriuretic peptide (ANP.emd), previously shown to be expressed in and released from prolactin-containing vesicles. We used stimulating solutions containing different [Ca 2+ ] to evoke vesicle peptide discharge, which appeared similar in membrane lawns and in intact stimulated lactotrophs. All vesicles examined discharged peptides in a subquantal manner, either exhibiting a unitary or sequential time course. In the membrane lawns, the unitary vesicle peptide discharge was predominant and slightly slower than that recorded in intact cells, but with a shorter delay with respect to the stimulation onset. This study revealed directly that Ca 2+ triggers peptide discharge from docked single vesicles in the membrane lawns with a half-maximal response of ∼8 µM [Ca 2+ ], consistent with previous whole-cell patch-clamp studies in endocrine cells where the rapid component of exocytosis, interpreted to represent docked vesicles, was fully activated at <10 µM [Ca 2+ ]. Interestingly, the sequential subquantal peptide vesicle discharge indicates that fluctuations between constricted and dilated fusion pore states are preserved in membrane lawns and that fusion pore regulation appears to be an autonomously controlled process.
Competing Interests: Declaration of Competing Interest None.
(Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
فهرسة مساهمة: Keywords: ANP.emd; Ca(2+) signalling; Confocal microscopy; Fusion pore; Lactotroph; Secretory vesicle; Stimulus-secretion coupling
المشرفين على المادة: SY7Q814VUP (Calcium)
9002-62-4 (Prolactin)
0 (Peptides)
تواريخ الأحداث: Date Created: 20221218 Date Completed: 20230110 Latest Revision: 20230227
رمز التحديث: 20230228
DOI: 10.1016/j.ceca.2022.102687
PMID: 36528978
قاعدة البيانات: MEDLINE
الوصف
تدمد:1532-1991
DOI:10.1016/j.ceca.2022.102687