دورية أكاديمية
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Dietary higenamine from Annonaceae family fruits as a possible source of unintentional doping.

التفاصيل البيبلوغرافية
العنوان: Dietary higenamine from Annonaceae family fruits as a possible source of unintentional doping.
المؤلفون: Muniz-Santos R; Laboratory of Protein Biochemistry, The Federal University of State of Rio de Janeiro (UNIRIO), Av. Pasteur, 296 - Urca, Rio de Janeiro, RJ, Brazil. Electronic address: renanmuniz@edu.unirio.br., Avezum J; Bichara e Motta Advogados, Av. Delfim Moreira, 120, Leblon, Rio de Janeiro, RJ, Brazil. Electronic address: juliana.avezum@bicharaemotta.com., Abidão-Neto B; Bichara e Motta Advogados, Av. Delfim Moreira, 120, Leblon, Rio de Janeiro, RJ, Brazil. Electronic address: bichara@bicharaemotta.com.br., Cameron LC; Laboratory of Protein Biochemistry, The Federal University of State of Rio de Janeiro (UNIRIO), Av. Pasteur, 296 - Urca, Rio de Janeiro, RJ, Brazil. Electronic address: cameron@unirio.br.
المصدر: Forensic science international [Forensic Sci Int] 2023 Jan; Vol. 342, pp. 111539. Date of Electronic Publication: 2022 Dec 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Ireland Country of Publication: Ireland NLM ID: 7902034 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-6283 (Electronic) Linking ISSN: 03790738 NLM ISO Abbreviation: Forensic Sci Int Subsets: MEDLINE
أسماء مطبوعة: Publication: Limerick : Elsevier Science Ireland
Original Publication: Lausanne, Elsevier Sequoia.
مواضيع طبية MeSH: Fruit* , Annonaceae*, Male ; Humans ; Young Adult ; Adult ; Tandem Mass Spectrometry/methods ; Diet ; Chromatography, Liquid
مستخلص: Members of the genus Aconitum have been used for millennia, both as poisons and medicines, in Eastern culture. Higenamine has non-selective beta-agonist effects, activating both β 1 and β 2 adrenoreceptors, and is present in a variety of plants. The World Anti-Doping Agency has banned Higenamine both in competition and out of competition. Due to the common uses of higenamine in Brazilian culture, both as medicine and food, we studied the urinary concentrations of higenamine after the consumption of fruits of the Annona genus. We evaluated whether the ingestion of these fruits has the potential to cause anti-doping code violations. We measured higenamine concentrations for a 72 h period in the urine of ten healthy, physically active males (age 20-30; weight 70-80 kg; not consuming supplements or medications) after eating a unique meal containing fruits. Fruit consumption ranges were: Carica papaya (control) 348 ± 98 g; A. muricata 450 ± 282 g; and A. squamosa 314 ± 60 g. (all mean± SD). Higenamine was measured using ultra-performance liquid chromatography coupled with electrospray-tandem mass spectrometry. The appearance of urinary higenamine occurred within the first 12 h after eating A. muricata (n = 3), and the maximum concentration found was 1.9 ng/mL. The ingestion of A. squamosa has also been shown to cause higenamine urinary excretion. The elimination kinetics of the subjects who ingested A. squamosa (n = 4) were different from each other. After ingestion of the control fruit, C. papaya, we detected no higenamine in the urine of any participants (n = 3). Although the kinetics varied by individuals and fruits, A. muricata ingestion produced higher higenamine excretion; however, the A. squamosa portion weighed ∼66 % of the A. muricata portion. We conclude that eating Annonaceae family fruits cause detectable higenamine excretion. Conversely, single ingestion did not reach the WADA's threshold to cause adverse analytical findings.
Competing Interests: Declaration of Interest Juliana Avezum and Bichara Abidão-Neto are attorneys, and L. C. Cameron is a biochemist and consultant working in doping cases.
(Copyright © 2022. Published by Elsevier B.V.)
فهرسة مساهمة: Keywords: Aconitum; Beta-agonist; Herbal medicine; Lotus; Norcoclaurine; Pharmacokinetics; Supplement; WADA
تواريخ الأحداث: Date Created: 20221218 Date Completed: 20230110 Latest Revision: 20230111
رمز التحديث: 20240628
DOI: 10.1016/j.forsciint.2022.111539
PMID: 36529085
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-6283
DOI:10.1016/j.forsciint.2022.111539