دورية أكاديمية
أعرض في EDS

Differential bone adaptation to mechanical unloading and reloading in young, old, and osteocyte deficient mice.

التفاصيل البيبلوغرافية
العنوان: Differential bone adaptation to mechanical unloading and reloading in young, old, and osteocyte deficient mice.
المؤلفون: Cunningham HC; University of California Davis Health, Department of Orthopaedic Surgery, 2700 Stockton Blvd, Suite 2301, Sacramento, CA 95817, United States of America., Orr S; University of California Davis Health, Department of Orthopaedic Surgery, 2700 Stockton Blvd, Suite 2301, Sacramento, CA 95817, United States of America., Murugesh DK; Lawrence Livermore National Laboratory, 7000 East Avenue, L-452, Livermore, CA 94550, United States of America., Hsia AW; University of California Davis Health, Department of Orthopaedic Surgery, 2700 Stockton Blvd, Suite 2301, Sacramento, CA 95817, United States of America., Osipov B; University of California Davis Health, Department of Orthopaedic Surgery, 2700 Stockton Blvd, Suite 2301, Sacramento, CA 95817, United States of America., Go L; University of California San Francisco, Department of Radiology & Biomedical Imaging, 185 Berry Street, Bldg B, San Francisco, CA 94158, United States of America., Wu PH; University of California San Francisco, Department of Radiology & Biomedical Imaging, 185 Berry Street, Bldg B, San Francisco, CA 94158, United States of America., Wong A; University of California Davis, School of Veterinary Medicine, 1285 Veterinary Medicine Dr, Bldg VM3A, Rm 4206, Davis, CA 95616, United States of America., Loots GG; University of California Davis Health, Department of Orthopaedic Surgery, 2700 Stockton Blvd, Suite 2301, Sacramento, CA 95817, United States of America; Lawrence Livermore National Laboratory, 7000 East Avenue, L-452, Livermore, CA 94550, United States of America., Kazakia GJ; University of California San Francisco, Department of Radiology & Biomedical Imaging, 185 Berry Street, Bldg B, San Francisco, CA 94158, United States of America., Christiansen BA; University of California Davis Health, Department of Orthopaedic Surgery, 2700 Stockton Blvd, Suite 2301, Sacramento, CA 95817, United States of America. Electronic address: bchristiansen@ucdavis.edu.
المصدر: Bone [Bone] 2023 Feb; Vol. 167, pp. 116646. Date of Electronic Publication: 2022 Dec 16.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: United States NLM ID: 8504048 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2763 (Electronic) Linking ISSN: 18732763 NLM ISO Abbreviation: Bone Subsets: MEDLINE
أسماء مطبوعة: Publication: New York : Elsevier Science
Original Publication: Elmsford, NY : Pergamon Press, c1985-
مواضيع طبية MeSH: Osteocytes*/metabolism , Bone and Bones*, Mice ; Animals ; Cortical Bone ; Femur/metabolism ; Hindlimb Suspension ; Mice, Transgenic
مستخلص: Mechanical unloading causes rapid loss of bone structure and strength, which gradually recovers after resuming normal loading. However, it is not well established how this adaptation to unloading and reloading changes with age. Clinically, elderly patients are more prone to musculoskeletal injury and longer periods of bedrest, therefore it is important to understand how periods of disuse will affect overall skeletal health of aged subjects. Bone also undergoes an age-related decrease in osteocyte density, which may impair mechanoresponsiveness. In this study, we examined bone adaptation during unloading and subsequent reloading in mice. Specifically, we examined the differences in bone adaptation between young mice (3-month-old), old mice (18-month-old), and transgenic mice that exhibit diminished osteocyte density at a young age (3-month-old BCL-2 transgenic mice). Mice underwent 14 days of hindlimb unloading followed by up to 14 days of reloading. We analyzed trabecular and cortical bone structure in the femur, mechanical properties of the femoral cortical diaphysis, osteocyte density and cell death in cortical bone, and serum levels of inflammatory cytokines. We found that young mice lost ~10% cortical bone volume and 27-42% trabecular bone volume during unloading and early reloading, with modest recovery of metaphyseal trabecular bone and near total recovery of epiphyseal trabecular bone, but no recovery of cortical bone after 14 days of reloading. Old mice lost 12-14% cortical bone volume and 35-50% trabecular bone volume during unloading and early reloading but had diminished recovery of trabecular bone during reloading and no recovery of cortical bone. In BCL-2 transgenic mice, no cortical bone loss was observed during unloading or reloading, but 28-31% trabecular bone loss occurred during unloading and early reloading, with little to no recovery during reloading. No significant differences in circulating inflammatory cytokine levels were observed due to unloading and reloading in any of the experimental groups. These results illustrate important differences in bone adaptation in older and osteocyte deficient mice, suggesting a possible period of vulnerability in skeletal health in older subjects during and following a period of disuse that may affect skeletal health in elderly patients.
Competing Interests: Declaration of competing interest The authors have no conflicts of interest to disclose.
(Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: R01 AR071459 United States AR NIAMS NIH HHS; R01 AR076159 United States AR NIAMS NIH HHS; R01 AR075013 United States AR NIAMS NIH HHS; P30 DK098722 United States DK NIDDK NIH HHS; T32 AR079099 United States AR NIAMS NIH HHS; P30 AR075055 United States AR NIAMS NIH HHS
فهرسة مساهمة: Keywords: Aging; Bone adaptation; Cortical porosity; Hindlimb unloading; Osteocytes
تواريخ الأحداث: Date Created: 20221218 Date Completed: 20230109 Latest Revision: 20240607
رمز التحديث: 20240607
مُعرف محوري في PubMed: PMC10077944
DOI: 10.1016/j.bone.2022.116646
PMID: 36529445
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-2763
DOI:10.1016/j.bone.2022.116646