دورية أكاديمية
أعرض في EDS

1D micro-nanopatterned integrin ligand surfaces for directed cell movement.

التفاصيل البيبلوغرافية
العنوان: 1D micro-nanopatterned integrin ligand surfaces for directed cell movement.
المؤلفون: Levario-Diaz V; Department of Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, Germany., Alvarado RE; Department of Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, Germany., Rodriguez-Quinteros CM; Department of Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, Germany., Fink A; Department of Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, Germany., Christian J; Department of Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, Germany., Feng W; Department of Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, Germany.; College of Polymer Science and Engineering, Sichuan University, Chengdu, China., Cavalcanti-Adam EA; Department of Cellular Biophysics, Max Planck Institute for Medical Research, Heidelberg, Germany.
المصدر: Frontiers in cell and developmental biology [Front Cell Dev Biol] 2022 Dec 02; Vol. 10, pp. 972624. Date of Electronic Publication: 2022 Dec 02 (Print Publication: 2022).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Media S.A Country of Publication: Switzerland NLM ID: 101630250 Publication Model: eCollection Cited Medium: Print ISSN: 2296-634X (Print) Linking ISSN: 2296634X NLM ISO Abbreviation: Front Cell Dev Biol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Media S.A., [2013]-
مستخلص: Cell-extracellular matrix (ECM) adhesion mediated by integrins is a highly regulated process involved in many vital cellular functions such as motility, proliferation and survival. However, the influence of lateral integrin clustering in the coordination of cell front and rear dynamics during cell migration remains unresolved. For this purpose, we describe a novel protocol to fabricate 1D micro-nanopatterned stripes by integrating the block copolymer micelle nanolithography (BCMNL) technique and maskless near UV lithography-based photopatterning. The photopatterned 10 μm-wide stripes consist of a quasi-perfect hexagonal arrangement of gold nanoparticles, decorated with the RGD (arginine-glycine-aspartate) motif for single integrin heterodimer binding, and placed at a distance of 50, 80, and 100 nm to regulate integrin clustering and focal adhesion dynamics. By employing time-lapse microscopy and immunostaining, we show that the displacement and speed of fibroblasts changes according to the nanoscale spacing of adhesion sites. We found that as the lateral spacing of adhesive peptides increased, fibroblast morphology was more elongated. This was accompanied by a decreased formation of mature focal adhesions and stress fibers, which increased cell displacement and speed. These results provide new insights into the migratory behavior of fibroblasts in 1D environments and our protocol offers a new platform to design and manufacture confined environments in 1D for integrin-mediated cell adhesion.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2022 Levario-Diaz, Alvarado, Rodriguez-Quinteros, Fink, Christian, Feng and Cavalcanti-Adam.)
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فهرسة مساهمة: Keywords: cell adhesion; focal adhesion (FA); integrins; micropatterning of surface; nanotechnology/nanomaterials
تواريخ الأحداث: Date Created: 20221219 Latest Revision: 20221222
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9755580
DOI: 10.3389/fcell.2022.972624
PMID: 36531964
قاعدة البيانات: MEDLINE
الوصف
تدمد:2296-634X
DOI:10.3389/fcell.2022.972624