دورية أكاديمية
أعرض في EDS

New substances of Equisetum hyemale L. extracts and their in vivo antitumoral effect against oral squamous cell carcinoma.

التفاصيل البيبلوغرافية
العنوان: New substances of Equisetum hyemale L. extracts and their in vivo antitumoral effect against oral squamous cell carcinoma.
المؤلفون: de Queiroz LN; Programa de Pós-Graduação em Ciências Aplicadas a Produtos para Saúde, Faculdade de Farmácia, Universidade Federal Fluminense, Niterói, RJ, Brazil. Electronic address: lucasnicolaunf@gmail.com., Da Fonseca ACC; Programa de Pós-graduação em Odontologia, Instituto de Saúde de Nova Friburgo, Universidade Federal Fluminense, CEP 28625-650, Nova Friburgo, RJ, Brazil. Electronic address: fonseca.anna@gmail.com., Wermelinger GF; Departamento de Ciência Básica, Instituto de Saúde de Nova Friburgo, Universidade Federal Fluminense, CEP 28625-650, Nova Friburgo, RJ, Brazil. Electronic address: guilhermefwermelinger@gmail.com., da Silva DPD; Departamento de Ciência Básica, Instituto de Saúde de Nova Friburgo, Universidade Federal Fluminense, CEP 28625-650, Nova Friburgo, RJ, Brazil. Electronic address: diegopdutra@hotmail.com., Pascoal ACRF; Departamento de Ciência Básica, Instituto de Saúde de Nova Friburgo, Universidade Federal Fluminense, CEP 28625-650, Nova Friburgo, RJ, Brazil. Electronic address: aislanfagundes@id.uff.br., Sawaya ACHF; Faculdade de Ciências Farmacêuticas, Universidade Estadual de Campinas, Campinas, São Paulo, CEP 13083-872, Brazil. Electronic address: alexandra.sawaya@fcf.unicamp.br., de Almeida ECP; Departamento de Ciência Básica, Instituto de Saúde de Nova Friburgo, Universidade Federal Fluminense, CEP 28625-650, Nova Friburgo, RJ, Brazil. Electronic address: elancardozo@id.uff.br., do Amaral BS; Instituto Federal de Educação, Ciência e Tecnologia de São Paulo, São Paulo, CEP 05110-000, Brazil., de Lima Moreira D; Laboratório de Produtos Naturais, Departamento de Pesquisa, Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rio de Janeiro, CEP 22460-030, Brazil. Electronic address: davysonmoreira@hotmail.com., Robbs BK; Departamento de Ciência Básica, Instituto de Saúde de Nova Friburgo, Universidade Federal Fluminense, CEP 28625-650, Nova Friburgo, RJ, Brazil. Electronic address: brunokr@id.uff.br.
المصدر: Journal of ethnopharmacology [J Ethnopharmacol] 2023 Mar 01; Vol. 303, pp. 116043. Date of Electronic Publication: 2022 Dec 16.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Sequoia Country of Publication: Ireland NLM ID: 7903310 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-7573 (Electronic) Linking ISSN: 03788741 NLM ISO Abbreviation: J Ethnopharmacol Subsets: MEDLINE
أسماء مطبوعة: Publication: Limerick : Elsevier Sequoia
Original Publication: Lausanne, Elsevier Sequoia.
مواضيع طبية MeSH: Equisetum*/chemistry , Carcinoma, Squamous Cell*/drug therapy , Mouth Neoplasms*/drug therapy , Head and Neck Neoplasms*, Mice ; Humans ; Animals ; Plant Extracts/pharmacology ; Plant Extracts/therapeutic use ; Plant Extracts/chemistry ; Squamous Cell Carcinoma of Head and Neck ; Tandem Mass Spectrometry ; Mice, Nude ; Ethanol ; Phytochemicals/pharmacology ; Flavonoids
مستخلص: Ethnobotanical Relevance: Equisetum hyemale is used in traditional medicine as an anti-inflammatory, antioxidant, diuretic and anticancer agent. Recent studies have observed antiproliferative activity of this species in some tumor cell lines.
Aim of the Study: The aim of this study was to evaluate the antiproliferative activity of the ethanol extract of E. hyemale and its partitions in oral squamous carcinoma cell lines, the death pathways induced by the most active partition, the acute toxicity and therapeutic activity, and the identification of the main compounds.
Materials and Methods: The ethanol crude extract was prepared from the stems of E. hyemale and partitions were obtained from this extract with n-hexane, dichloromethane and ethyl acetate. Cytotoxicity assays were performed using MTT on human oral tumor lines SCC-9, SCC4 and SCC-25, and normal primary fibroblasts. The main pathways of programmed cell death were analyzed. Acute toxicity in mice was performed using the most active partition, ethyl acetate. Antitumor activity was accessed in xenotransplants grafts of SCC-9 cells in Balb/nude mice. Phytochemical analysis was performed using UHPLC-MS/MS and dereplication was done using Global Natural Product Social Molecular Networking (GNPS) analysis.
Results: Ethanol extract, n-hexane and ethyl acetate partitions showed dose-dependent activity and selectivity towards oral tumor cells, with the ethyl acetate being the most bioactive. This medium polarity partition was shown to induce tumor cell death through apoptosis due to the presence of activated caspase 3/7, DNA fragmentation, chromatin condensation and phosphatidylserine exposure. The ethyl acetate partition also produced low toxicity in mice, provoking mild hepatic changes, but without causing necrosis and significantly reduced tumors volume and weight in xenotransplants of SCC-9 cells. Phytochemical analysis allowed identification of kaempferol glycosides and cinnamic acid derivatives previously described for E. hyemale. In addition it was possible to identify 6 new non-glycolyzed flavonoids 5-Hydroxy-3',4',7,8-tetramethoxyflavone (14), 5,4'-Dihydroxy-7,8,3'-trimethoxyflavone (15), 5,7-Dihydroxy-3',4'-dimethoxyflavone (16), 3',4,5,7-Tretramethoxyflavone (17), 5-Hydroxy-3'4',7-trimethoxyflavone (18), and 5,4'-Dihydroxy-3'-7'-dimethoxyflavone (19); besides 5 compounds already determined to be cytotoxic in other species, Isoferulic acid (1), Ferulic acid (2), Atractylenolide III (6), Dihydroxy-3',4'-dimethoxyflavone (16), and 5-Hydroxy-3'4 ',7-trimethoxyflavone (18).
Conclusion: The results indicate that the E. hyemale extract and partitions inhibited 3 different cell lines of OSCC in a highly selective nontoxic way by inducing apoptosis of the cells. We identified 6 new non-glycosylated flavonoids and 5 other substances in this species.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2022 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Apoptosis; Cancer therapy; Cytotoxicity; Equisetum hyemale; Phytochemical analysis
المشرفين على المادة: 0 (Plant Extracts)
76845O8NMZ (ethyl acetate)
2DDG612ED8 (n-hexane)
3K9958V90M (Ethanol)
0 (Phytochemicals)
0 (Flavonoids)
تواريخ الأحداث: Date Created: 20221219 Date Completed: 20221228 Latest Revision: 20230103
رمز التحديث: 20231215
DOI: 10.1016/j.jep.2022.116043
PMID: 36535330
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-7573
DOI:10.1016/j.jep.2022.116043