دورية أكاديمية
Impact of Hyaluronic Acid on the Viability of Mesenchymal Cells Derived from Adipose Tissue Grown in Collagen Type I/III Membrane.
| العنوان: | Impact of Hyaluronic Acid on the Viability of Mesenchymal Cells Derived from Adipose Tissue Grown in Collagen Type I/III Membrane. |
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| المؤلفون: | Kaleka CC; Departamento de Ortopedia e Traumatologia, Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Debieux P; Departamento de Ortopedia e Traumatologia, Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Antonioli E; Ortopedia Multiprofissional, Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Zucconi E; Laboratório StemCorp de Tecnologia em Células-Tronco, São Paulo, SP, Brasil., Cohen M; Departamento de Ortopedia e Traumatologia, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brasil.; Programa de Pós-graduação em Ciências da Saúde, Hospital Israelita Albert Einstein, São Paulo, SP, Brasil., Ferretti M; Programa de Pós-graduação em Ciências da Saúde, Hospital Israelita Albert Einstein, São Paulo, SP, Brasil. |
| المصدر: | Revista brasileira de ortopedia [Rev Bras Ortop (Sao Paulo)] 2022 Feb 09; Vol. 57 (6), pp. 1022-1029. Date of Electronic Publication: 2022 Feb 09 (Print Publication: 2022). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Thieme Country of Publication: Germany NLM ID: 0123326 Publication Model: eCollection Cited Medium: Print ISSN: 0102-3616 (Print) Linking ISSN: 01023616 NLM ISO Abbreviation: Rev Bras Ortop (Sao Paulo) Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : [Stuttgart] : Thieme Original Publication: São Paulo : Sociedade Brasileira de Ortopedia e Traumatologia |
| مستخلص: | Objective To evaluate in vitro the viability of mesenchymal stem cells derived from adipose tissue (AD-MSCs) in different commercial solutions of hyaluronic acid (HA) before and after being sowed in collagen I/III membrane. Methods In the first stage, the interaction between AD-MSCs was analyzed with seven different commercial products of HA, phosphate buffered saline (PBS), and bovine fetal serum (BFS), performed by counting living and dead cells after 24, 48 and 72 hours. Five products with a higher number of living cells were selected and the interaction between HA with AD-MSCs and type I/III collagen membrane was evaluated by counting living and dead cells in the same time interval (24, 48 and 72 hours). Results In both situations analyzed (HA + AD-MSCs and HA + AD-MSCs + membrane), BFS presented the highest percentage of living cells after 24, 48 and 72 hours, a result higher than that of HA. Conclusion The association of HA with AD-MSCs, with or without membrane, showed no superiority in cell viability when compared with BFS. Competing Interests: Conflito de Interesses Os autores declaram não haver conflito de interesses. (Sociedade Brasileira de Ortopedia e Traumatologia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. ( https://creativecommons.org/licenses/by-nc-nd/4.0/ ).) |
| References: | Arthroscopy. 2010 Jun;26(6):841-52. (PMID: 20511044) Rev Esp Cir Ortop Traumatol. 2015 Jul-Aug;59(4):215-21. (PMID: 25481699) Curr Rev Musculoskelet Med. 2015 Mar;8(1):1-8. (PMID: 25591739) Stem Cell Rev Rep. 2010 Dec;6(4):560-6. (PMID: 20821076) Rev Bras Ortop. 2018 Sep 21;53(6):733-739. (PMID: 30377608) Curr Stem Cell Res Ther. 2012 Mar;7(2):103-9. (PMID: 22023629) Cytotherapy. 2014 Apr;16(4):485-95. (PMID: 24119649) Stem Cells Int. 2016;2016:9364213. (PMID: 26981136) Cell Transplant. 2015;24(7):1221-32. (PMID: 24853696) N Engl J Med. 1994 Oct 6;331(14):889-95. (PMID: 8078550) Front Bioeng Biotechnol. 2020 Mar 31;8:243. (PMID: 32296689) Stem Cells Int. 2015;2015:421253. (PMID: 26257790) J Clin Med. 2019 Mar 21;8(3):. (PMID: 30901900) Stem Cells Transl Med. 2017 Jun;6(6):1445-1451. (PMID: 28452204) Cell Commun Signal. 2011 May 14;9:12. (PMID: 21569606) Arthritis Rheum. 2013 May;65(5):1271-81. (PMID: 23613363) Acta Biomater. 2014 Apr;10(4):1571-1580. (PMID: 23899481) Cytotherapy. 2006;8(4):315-7. (PMID: 16923606) Rev Bras Ortop. 2018 Aug 02;53(5):557-563. (PMID: 30245994) J Cell Biochem. 2006 Aug 1;98(5):1076-84. (PMID: 16619257) |
| فهرسة مساهمة: | Keywords: articular; cartilage; hyaluronic acid; mesenchymal stem cell transplantation; mesenchymal stem cells |
| تواريخ الأحداث: | Date Created: 20221221 Latest Revision: 20230214 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC9757957 |
| DOI: | 10.1055/s-0041-1740198 |
| PMID: | 36540744 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 0102-3616 |
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| DOI: | 10.1055/s-0041-1740198 |