دورية أكاديمية

Acute responsiveness to single leg cycling in adults with obesity.

التفاصيل البيبلوغرافية
العنوان: Acute responsiveness to single leg cycling in adults with obesity.
المؤلفون: Gries KJ; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA.; Department of Physical Therapy, School of Health Professions, Concordia University of Wisconsin, Mequon, Wisconsin, USA., Hart CR; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA.; Air Force Research Laboratory, 711th Human Performance Wing, Wright Patterson Air Force Base, Dayton, Ohio, USA., Kunz HE; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA., Ryan Z; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA., Zhang X; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA.; Department of Geriatrics, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai, China., Parvizi M; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA., Liu Y; Department of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota, USA., Dasari S; Department of Biomedical Statistics and Informatics, Mayo Clinic, Rochester, Minnesota, USA., Lanza IR; Endocrine Research Unit, Division of Endocrinology, Department of Internal Medicine, Mayo Clinic, Rochester, Minnesota, USA.
المصدر: Physiological reports [Physiol Rep] 2022 Dec; Vol. 10 (24), pp. e15539.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society Country of Publication: United States NLM ID: 101607800 Publication Model: Print Cited Medium: Internet ISSN: 2051-817X (Electronic) Linking ISSN: 2051817X NLM ISO Abbreviation: Physiol Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [Malden MA] : published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society, 2013-
مواضيع طبية MeSH: Leg* , Muscle, Skeletal*/metabolism, Adult ; Humans ; Muscle Proteins/metabolism ; Obesity/metabolism ; Quadriceps Muscle/metabolism ; Male ; Female
مستخلص: Obesity is associated with several skeletal muscle impairments which can be improved through an aerobic exercise prescription. The possibility that exercise responsiveness is diminished in people with obesity has been suggested but not well-studied. The purpose of this study was to investigate how obesity influences acute exercise responsiveness in skeletal muscle and circulating amino metabolites. Non-obese (NO; n = 19; 10F/9M; BMI = 25.1 ± 2.8 kg/m 2 ) and Obese (O; n = 21; 14F/7M; BMI = 37.3 ± 4.6 kg/m 2 ) adults performed 30 min of single-leg cycling at 70% of VO 2 peak. 13 C 6 -Phenylalanine was administered intravenously for muscle protein synthesis measurements. Serial muscle biopsies (vastus lateralis) were collected before exercise and 3.5- and 6.5-h post-exercise to measure protein synthesis and gene expression. Targeted plasma metabolomics was used to quantitate amino metabolites before and 30 and 90 min after exercise. The exercise-induced fold change in mixed muscle protein synthesis trended (p = 0.058) higher in NO (1.28 ± 0.54-fold) compared to O (0.95 ± 0.42-fold) and was inversely related to BMI (R 2  = 0.140, p = 0.027). RNA sequencing revealed 331 and 280 genes that were differentially expressed after exercise in NO and O, respectively. Gene set enrichment analysis showed O had six blunted pathways related to metabolism, cell to cell communication, and protein turnover after exercise. The circulating amine response further highlighted dysregulations related to protein synthesis and metabolism in adults with obesity at the basal state and in response to the exercise bout. Collectively, these data highlight several unique pathways in individuals with obesity that resulted in a modestly blunted exercise response.
(© 2022 The Authors. Physiological Reports published by Wiley Periodicals LLC on behalf of The Physiological Society and the American Physiological Society.)
References: J Clin Endocrinol Metab. 2009 Aug;94(8):3044-50. (PMID: 19470633)
Nat Commun. 2020 Jan 24;11(1):470. (PMID: 31980607)
Am J Physiol Endocrinol Metab. 2019 Sep 1;317(3):E460-E472. (PMID: 31265326)
J Nutr. 2006 Feb;136(2):533S-537S. (PMID: 16424142)
Can J Appl Physiol. 1995 Dec;20(4):480-6. (PMID: 8563679)
Nat Rev Endocrinol. 2014 Dec;10(12):723-36. (PMID: 25287287)
Clin Physiol. 1985 Apr;5(2):155-60. (PMID: 2859940)
Physiol Rep. 2022 Dec;10(24):e15539. (PMID: 36541258)
Am J Clin Nutr. 2016 Oct;104(4):1014-1022. (PMID: 27604771)
Front Nutr. 2019 Jun 13;6:87. (PMID: 31263701)
J Clin Endocrinol Metab. 2017 Sep 1;102(9):3535-3545. (PMID: 28911148)
Physiol Rep. 2018 Jul;6(14):e13799. (PMID: 30009507)
J Physiol. 2008 Aug 1;586(15):3701-17. (PMID: 18556367)
Diabetes Care. 2009 Nov;32 Suppl 2:S157-63. (PMID: 19875544)
Obesity (Silver Spring). 2022 May;30(5):1091-1104. (PMID: 35470975)
Metabolism. 2005 Aug;54(8):1048-55. (PMID: 16092055)
J Physiol. 2005 Sep 15;567(Pt 3):1021-33. (PMID: 16002437)
Am J Physiol Endocrinol Metab. 2021 Jul 1;321(1):E105-E121. (PMID: 33998291)
J Physiol. 1999 Jan 1;514 ( Pt 1):293-302. (PMID: 9831734)
J Appl Physiol (1985). 2007 Jul;103(1):388-95. (PMID: 17303713)
FASEB J. 1998 Oct;12(13):1379-89. (PMID: 9761781)
J Cell Mol Med. 2020 May;24(9):5352-5362. (PMID: 32239667)
Cell Metab. 2012 Dec 5;16(6):777-88. (PMID: 23217257)
J Physiol. 2000 Aug 1;526 Pt 3:663-9. (PMID: 10922016)
Diabetes. 2013 Aug;62(8):2717-27. (PMID: 23610057)
Diabetes Care. 2001 May;24(5):933-41. (PMID: 11347757)
PLoS One. 2010 May 10;5(5):e10538. (PMID: 20479934)
Diabetologia. 1980 Jun;18(6):463-9. (PMID: 6998811)
Biomed Res Int. 2015;2015:418681. (PMID: 25821801)
J Mass Spectrom. 2013 Feb;48(2):269-75. (PMID: 23378099)
Cell Metab. 2014 Jan 7;19(1):96-108. (PMID: 24411942)
BMC Bioinformatics. 2006 Mar 09;7:123. (PMID: 16526949)
J Nutr. 2019 Sep 1;149(9):1533-1542. (PMID: 31174213)
Am J Physiol Endocrinol Metab. 2013 Jun 15;304(12):E1391-403. (PMID: 23632634)
J Physiol. 2009 Nov 15;587(Pt 22):5483-92. (PMID: 19752122)
Cell Calcium. 2016 Oct;60(4):235-44. (PMID: 27292137)
Aging (Albany NY). 2017 Apr;9(4):1096-1129. (PMID: 28379838)
J Clin Endocrinol Metab. 2015 Jan;100(1):43-52. (PMID: 25412336)
J Clin Endocrinol Metab. 2014 Dec;99(12):E2574-83. (PMID: 25222757)
J Biol Chem. 1967 May 10;242(9):2278-82. (PMID: 4290225)
Int J Sports Med. 1989 Aug;10(4):233-6. (PMID: 2606590)
FASEB J. 2001 Feb;15(2):475-82. (PMID: 11156963)
Am J Physiol Endocrinol Metab. 2008 Mar;294(3):E607-14. (PMID: 18182465)
Front Physiol. 2021 Aug 02;12:702742. (PMID: 34408662)
Diabetes. 2005 Jan;54(1):8-14. (PMID: 15616005)
Nat Rev Endocrinol. 2019 May;15(5):288-298. (PMID: 30814686)
Front Nutr. 2021 May 05;8:615849. (PMID: 34026802)
J Exp Biol. 1991 Oct;160:149-65. (PMID: 1960512)
Br J Sports Med. 2019 Sep;53(18):1141-1153. (PMID: 30862704)
J Physiol. 2001 Oct 1;536(Pt 1):273-82. (PMID: 11579175)
Am J Physiol Endocrinol Metab. 2012 Jul 1;303(1):E82-9. (PMID: 22510711)
Front Physiol. 2019 Sep 25;10:1240. (PMID: 31611815)
Science. 2001 Nov 23;294(5547):1704-8. (PMID: 11679633)
Am J Physiol Endocrinol Metab. 2005 May;288(5):E883-91. (PMID: 15613683)
J Clin Invest. 1982 Apr;69(4):785-93. (PMID: 6804492)
Methods. 2001 Dec;25(4):402-8. (PMID: 11846609)
J Physiol. 2018 Nov;596(21):5119-5133. (PMID: 30113718)
J Physiol. 2003 Jan 15;546(Pt 2):327-35. (PMID: 12527721)
Am J Physiol Endocrinol Metab. 2006 Dec;291(6):E1220-7. (PMID: 16835401)
Am J Clin Nutr. 2006 Sep;84(3):475-82. (PMID: 16960159)
Diabetologia. 2009 Sep;52(9):1789-97. (PMID: 19370339)
Skelet Muscle. 2011 Mar 02;1(1):11. (PMID: 21798089)
J Clin Invest. 2013 Oct;123(10):4309-17. (PMID: 24091325)
Integr Med Res. 2014 Dec;3(4):172-179. (PMID: 28664094)
Front Physiol. 2020 Feb 13;11:91. (PMID: 32116795)
Am J Physiol Regul Integr Comp Physiol. 2007 Mar;292(3):R1271-8. (PMID: 17095651)
Obes Res. 2002 Jul;10(7):575-84. (PMID: 12105277)
J Clin Invest. 2009 Mar;119(3):573-81. (PMID: 19188683)
Am J Physiol Regul Integr Comp Physiol. 2010 Nov;299(5):R1254-62. (PMID: 20720176)
معلومات مُعتمدة: UL1 TR002377 United States TR NCATS NIH HHS
فهرسة مساهمة: Keywords: amino acid metabolism; exercise; protein synthesis; transcriptome
المشرفين على المادة: 0 (Muscle Proteins)
تواريخ الأحداث: Date Created: 20221221 Date Completed: 20221230 Latest Revision: 20240216
رمز التحديث: 20240216
مُعرف محوري في PubMed: PMC9768637
DOI: 10.14814/phy2.15539
PMID: 36541258
قاعدة البيانات: MEDLINE
الوصف
تدمد:2051-817X
DOI:10.14814/phy2.15539