دورية أكاديمية
Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry.
| العنوان: | Hyaluronan-cecropin B interactions studied by ultrasound velocimetry and isothermal titration calorimetry. |
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| المؤلفون: | Jugl A; Brno University of Technology, Faculty of Chemistry, Institute of Physical and Applied Chemistry, Purkyňova 118, Brno, Czech Republic. Electronic address: xcjugl@fch.vut.cz., Pekař M; Brno University of Technology, Faculty of Chemistry, Institute of Physical and Applied Chemistry, Purkyňova 118, Brno, Czech Republic. Electronic address: pekar@fch.vut.cz. |
| المصدر: | International journal of biological macromolecules [Int J Biol Macromol] 2023 Feb 01; Vol. 227, pp. 786-794. Date of Electronic Publication: 2022 Dec 19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 7909578 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0003 (Electronic) Linking ISSN: 01418130 NLM ISO Abbreviation: Int J Biol Macromol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Amsterdam : Elsevier Original Publication: Guildford, Eng., IPC Science and Technology Press. |
| مواضيع طبية MeSH: | Hyaluronic Acid* , Cecropins*, Calorimetry/methods ; Water/chemistry ; Rheology ; Thermodynamics |
| مستخلص: | Interactions between hyaluronan and the antimicrobial peptide cecropin B were studied in water and PBS using high-resolution ultrasonic spectroscopy and isothermal titration calorimetry. Although each technique is fundamentally different, they both gave identical results. It was found that the molecular weight of hyaluronan plays an important role in the interactions - in particular, the transition between the rod conformation and the random coil conformation. In water, interactions were saturated in a molar charge ratio of 1.5 and not 1.0 as expected. The later saturation of the interaction probably occurred either for steric reasons or due to the interaction between functional groups in the cecropin structure, which allowed complete dissociation of the antimicrobial peptide. In PBS, in contrast to water, no interactions were observed, irrespective of the molecular weight of hyaluronan. Thus, at a sufficiently high ionic strength, the interactions were suppressed. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cecropin B; High-resolution ultrasonic spectroscopy; Hyaluronan; Interaction; Isothermal titration calorimetry |
| المشرفين على المادة: | 9004-61-9 (Hyaluronic Acid) 0 (Cecropins) 059QF0KO0R (Water) |
| تواريخ الأحداث: | Date Created: 20221222 Date Completed: 20230112 Latest Revision: 20230112 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.ijbiomac.2022.12.144 |
| PMID: | 36549616 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0003 |
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| DOI: | 10.1016/j.ijbiomac.2022.12.144 |