دورية أكاديمية
Antiulcerogenic Potential of the Ethanolic Extract of Ceiba speciosa (A. St.-Hil.) Ravenna Evaluated by In Vitro and In Vivo Studies.
العنوان: | Antiulcerogenic Potential of the Ethanolic Extract of Ceiba speciosa (A. St.-Hil.) Ravenna Evaluated by In Vitro and In Vivo Studies. |
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المؤلفون: | Dörr JA; Graduate Program in Biotechnology (PPGBiotec), University of Vale do Taquari-Univates, Av. Avelino Talini, 171, Lajeado 95914-014, RS, Brazil., Majolo F; Graduate Program in Medicine Sciences (PPGCM), University of Vale do Taquari-Univates, Av. Avelino Talini, 171, Lajeado 95914-014, RS, Brazil., Bortoluzzi L; Graduate Program in Biotechnology (PPGBiotec), University of Vale do Taquari-Univates, Av. Avelino Talini, 171, Lajeado 95914-014, RS, Brazil., de Vargas EZ; Graduate Program in Biotechnology (PPGBiotec), University of Vale do Taquari-Univates, Av. Avelino Talini, 171, Lajeado 95914-014, RS, Brazil., Silva J; MARE-Marine and Environmental Sciences Centre/ARNET-Aquatic Research Network, Polytechnic of Leiria, 2520-630 Peniche, Portugal., Pasini M; Graduate Program in Biotechnology (PPGBiotec), University of Vale do Taquari-Univates, Av. Avelino Talini, 171, Lajeado 95914-014, RS, Brazil., Stoll SN; Graduate Program in Biotechnology (PPGBiotec), University of Vale do Taquari-Univates, Av. Avelino Talini, 171, Lajeado 95914-014, RS, Brazil., da Rosa RL; Institute of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre 91501-970, RS, Brazil., Figueira MM; Graduate Program in Pharmaceutical Sciences, Natural Products Laboratory, Vila Velha University, Vila Velha 29102-920, ES, Brazil., Fronza M; Graduate Program in Pharmaceutical Sciences, Natural Products Laboratory, Vila Velha University, Vila Velha 29102-920, ES, Brazil., Beys-da-Silva WO; Institute of Pharmacy, Universidade Federal do Rio Grande do Sul, Porto Alegre 91501-970, RS, Brazil., Martins A; MARE-Marine and Environmental Sciences Centre/ARNET-Aquatic Research Network, Polytechnic of Leiria, 2520-630 Peniche, Portugal., Gaspar H; BioISI-Biosystems and Integrative Sciences Institute, Faculty of Sciences, University of Lisbon, 1749-016 Lisboa, Portugal., Pedrosa RP; MARE-Marine and Environmental Sciences Centre/ARNET-Aquatic Research Network, Polytechnic of Leiria, 2520-630 Peniche, Portugal., Laufer S; Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, Eberhard Karls Universität Tübingen, D 72076 Tuebingen, Germany.; Tuübingen Center for Academic Drug Discovery-TÜCADD, Eberhard Karls Universität Tübingen, D 72076 Tübingen, Germany.; Cluster of Excellence iFIT (EXC 2180) 'Image-Guided and Functionally Instructed Tumor Therapies', Eberhard Karls Universität Tübingen, D 72076 Tübingen, Germany., Goettert MI; Graduate Program in Biotechnology (PPGBiotec), University of Vale do Taquari-Univates, Av. Avelino Talini, 171, Lajeado 95914-014, RS, Brazil.; Graduate Program in Medicine Sciences (PPGCM), University of Vale do Taquari-Univates, Av. Avelino Talini, 171, Lajeado 95914-014, RS, Brazil.; Department of Pharmaceutical and Medicinal Chemistry, Institute of Pharmacy, Eberhard Karls Universität Tübingen, D 72076 Tuebingen, Germany. |
المصدر: | International journal of molecular sciences [Int J Mol Sci] 2022 Dec 09; Vol. 23 (24). Date of Electronic Publication: 2022 Dec 09. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
مواضيع طبية MeSH: | Anti-Ulcer Agents*/pharmacology , Ceiba*/chemistry , Plant Extracts*/chemistry , Plant Extracts*/pharmacology , Stomach Ulcer*/chemically induced , Stomach Ulcer*/drug therapy, Animals ; Humans ; Rats ; Antioxidants/pharmacology ; Carrageenan/adverse effects ; Tumor Necrosis Factor-alpha/metabolism ; Ulcer |
مستخلص: | Gastrointestinal diseases, such as peptic ulcers, are caused by a damage in the gastric mucosa provoked by several factors. This stomach injury is regulated by many inflammatory mediators and is commonly treated with proton-pump inhibitors, histamine H2 receptor blockers and antacids. However, various medicinal plants have demonstrated positive effects on gastric ulcer treatment, including plants of the Ceiba genus. The aim of this study was to evaluate the antiulcer and anti-inflammatory activities of the stem bark ethanolic extract of Ceiba speciosa (A. St.-Hil.) Ravenna. We performed a preliminary quantification of phenolic compounds by high-performance liquid chromatography-diode array detection (HPLC-DAD), followed by the prospection of other chemical groups through nuclear magnetic resonance (NMR) spectroscopy. A set of in vitro assays was used to evaluate the extract potential regarding its antioxidant activity (DPPH: 19.83 ± 0.34 µg/mL; TPC: 307.20 ± 6.20 mg GAE/g of extract), effects on cell viability and on the release of TNF-α in whole human blood. Additionally, in vivo assays were performed to evaluate the leukocyte accumulation and total protein quantification in carrageenan-induced air pouch, as well as the antiulcerogenic effect of the extract on an ethanol-induced ulcer in rats. The extract contains flavonoids and phenolic compounds, as well as sugars and quinic acid derivatives exhibiting potent antioxidant activity and low toxicity. The extract reduced the release of TNF-α in human blood and inhibited the activity of p38α (1.66 µg/mL), JAK3 (5.25 µg/mL), and JNK3 (8.34 µg/mL). Moreover, it reduced the leukocyte recruitment on the pouch exudate and the formation of edema, reverting the effects caused by carrageenan. The extract presented a significant prevention of ulcer formation and a higher reduction than the reference drug, Omeprazole. Therefore, C. speciosa extract has demonstrated relevant therapeutic potential for the treatment of gastric diseases, deserving the continuation of further studies to unveil the mechanisms of action of plant bioactive ingredients. |
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معلومات مُعتمدة: | 001 Coordenação de Aperfeicoamento de Pessoal de Nível Superior; 420381/2018-0. National Council for Scientific and Technological Development; UIDP/04292/2020; UIDB/04292/2020 MARE-Marine and Environmental Sciences Center; UIDP/Multi/04046/2020; UIDB/04046/2020 BioSystems and Integrative Sciences Institute; 2019.00277.CBM FCT/DRI/CAPES; 2019 Univates |
فهرسة مساهمة: | Keywords: Ceiba genus; anti-inflammatory; antioxidant activity; gastrointestinal diseases; inflammation; peptic ulcer |
المشرفين على المادة: | 0 (Anti-Ulcer Agents) 0 (Antioxidants) 9000-07-1 (Carrageenan) 0 (Plant Extracts) 0 (Tumor Necrosis Factor-alpha) |
تواريخ الأحداث: | Date Created: 20221223 Date Completed: 20230103 Latest Revision: 20230103 |
رمز التحديث: | 20230104 |
مُعرف محوري في PubMed: | PMC9779658 |
DOI: | 10.3390/ijms232415634 |
PMID: | 36555275 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1422-0067 |
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DOI: | 10.3390/ijms232415634 |