دورية أكاديمية
أعرض في EDS

Enhancing Techniques for Determining Inflammatory Edema Formation and Neutrophil Accumulation in Murine Skin.

التفاصيل البيبلوغرافية
العنوان: Enhancing Techniques for Determining Inflammatory Edema Formation and Neutrophil Accumulation in Murine Skin.
المؤلفون: Zarban AA; Section of Vascular Biology and Inflammation, British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, King's College London, London, United Kingdom.; Department of Pharmacology and Toxicology, College of Pharmacy, Jazan University, Jazan, Saudi Arabia., Chaudhry H; Section of Vascular Biology and Inflammation, British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, King's College London, London, United Kingdom., Maselli D; James Black Centre, British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, King's College London, London, United Kingdom., Kodji X; Agency for Science, Technology and Research (ASTAR) - Skin Research Institute of Singapore (SRIS), Singapore, Singapore., de Sousa Valente J; Section of Vascular Biology and Inflammation, British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, King's College London, London, United Kingdom., Joachim J; James Black Centre, British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, King's College London, London, United Kingdom., Trevelin SC; James Black Centre, British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, King's College London, London, United Kingdom., van Baardewijk J; Ray Global Education, Shanghai, China., Argunhan F; Section of Vascular Biology and Inflammation, British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, King's College London, London, United Kingdom., Ivetic A; James Black Centre, British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, King's College London, London, United Kingdom., Nandi M; School of Cancer & Pharmaceutical Sciences, Faculty of Life Sciences & Medicine, King's College London, London, United Kingdom., Brain SD; Section of Vascular Biology and Inflammation, British Heart Foundation Centre of Research Excellence, School of Cardiovascular and Metabolic Medicine & Sciences, King's College London, London, United Kingdom.
المصدر: JID innovations : skin science from molecules to population health [JID Innov] 2022 Sep 07; Vol. 3 (1), pp. 100154. Date of Electronic Publication: 2022 Sep 07 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V. on behalf of the Society for Investigative Dermatology Country of Publication: Netherlands NLM ID: 101776173 Publication Model: eCollection Cited Medium: Internet ISSN: 2667-0267 (Electronic) Linking ISSN: 26670267 NLM ISO Abbreviation: JID Innov Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: {Amsterdam] : Elsevier B.V. on behalf of the Society for Investigative Dermatology, [2021]-
مستخلص: Inflammatory edema formation and polymorphonuclear leukocyte (neutrophil) accumulation are common components of cutaneous vascular inflammation, and their assessment is a powerful investigative and drug development tool but typically requires independent cohorts of animals to assess each. We have established the use of a mathematical formula to estimate the ellipsoidal-shaped volume of the edematous wheal or bleb after intradermal injections of substances in mice pretreated intravenously with Evans blue dye (which binds to plasma albumin) to act as an edema marker. Whereas previous extraction of Evans blue dye with formamide is suitable for all strains of mice, we report this quicker and more reliable assessment of edema volume in situ. This therefore allows neutrophil accumulation to be assessed from the same mouse using the myeloperoxidase assay. Importantly, we examined the influence of Evans blue dye on the spectrometry readout at the wavelength at which myeloperoxidase activity is measured. The results indicate that it is feasible to quantify edema formation and neutrophil accumulation in the same mouse skin site. Thus, we show techniques that can assess edema formation and neutrophil accumulation at the same site in the same mouse, allowing paired measurements and reducing the total use of mice by 50%.
(© 2022 The Authors.)
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معلومات مُعتمدة: PG/19/14/34268 United Kingdom BHF_ British Heart Foundation
فهرسة مساهمة: Keywords: CGRP, calcitonin-gene related peptide; EB, Evans blue; MPO, myeloperoxidase; OD, optical density; SP, substance P; TMB, 3,3′,5,5′-tetramethylbenzidine; i.d., intradermally; i.v., intravenously
تواريخ الأحداث: Date Created: 20221223 Latest Revision: 20230322
رمز التحديث: 20230322
مُعرف محوري في PubMed: PMC9763761
DOI: 10.1016/j.xjidi.2022.100154
PMID: 36561914
قاعدة البيانات: MEDLINE
الوصف
تدمد:2667-0267
DOI:10.1016/j.xjidi.2022.100154