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Guidelines for DC preparation and flow cytometry analysis of mouse lymphohematopoietic tissues.

التفاصيل البيبلوغرافية
العنوان: Guidelines for DC preparation and flow cytometry analysis of mouse lymphohematopoietic tissues.
المؤلفون: Amon L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany., Dudziak D; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany.; Medical Immunology Campus Erlangen (MICE), Erlangen, Germany.; Deutsches Zentrum Immuntherapie (DZI), Germany.; Friedrich-Alexander University Erlangen-Nürnberg, Erlangen, Germany., Backer RA; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.; Institute for Molecular Medicine, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany., Clausen BE; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.; Institute for Molecular Medicine, Paul Klein Center for Immune Intervention, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany., Gmeiner C; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany., Heger L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany., Jacobi L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany., Lehmann CHK; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany.; Medical Immunology Campus Erlangen (MICE), Erlangen, Germany., Probst HC; Research Center for Immunotherapy (FZI), University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany.; Institute of Immunology, University Medical Center Mainz, Mainz, Germany., Seichter A; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany., Tchitashvili G; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany., Tochoedo NR; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany., Trapaidze L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany., Vurnek D; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Erlangen, Germany.
المصدر: European journal of immunology [Eur J Immunol] 2023 Dec; Vol. 53 (12), pp. e2249893. Date of Electronic Publication: 2022 Dec 23.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 1273201 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-4141 (Electronic) Linking ISSN: 00142980 NLM ISO Abbreviation: Eur J Immunol Subsets: MEDLINE
أسماء مطبوعة: Publication: <2005->: Weinheim : Wiley-VCH
Original Publication: Weinheim, Verlag Chemie GmbH.
مواضيع طبية MeSH: Dendritic Cells* , Thymus Gland*, Humans ; Flow Cytometry ; Spleen ; Phenotype
مستخلص: This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human DC from lymphoid organs, and various non-lymphoid tissues. Within this chapter, detailed protocols are presented that allow for the generation of single-cell suspensions from mouse lymphohematopoietic tissues including spleen, peripheral lymph nodes, and thymus, with a focus on the subsequent analysis of DC by flow cytometry. However, prepared single-cell suspensions can be subjected to other applications including sorting and cellular enrichment procedures, RNA sequencing, Western blotting, and many more. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.
(© 2022 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.)
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فهرسة مساهمة: Keywords: dendritic cells; high-parameter flow cytometry; mouse lymphoid tissues; myeloid cells; single-cell suspension preparation
تواريخ الأحداث: Date Created: 20221223 Date Completed: 20231220 Latest Revision: 20231220
رمز التحديث: 20231220
DOI: 10.1002/eji.202249893
PMID: 36563125
قاعدة البيانات: MEDLINE
الوصف
تدمد:1521-4141
DOI:10.1002/eji.202249893