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Guidelines for DC preparation and flow cytometric analysis of human lymphohematopoietic tissues.

التفاصيل البيبلوغرافية
العنوان: Guidelines for DC preparation and flow cytometric analysis of human lymphohematopoietic tissues.
المؤلفون: Heger L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Hartmannstraße 14, D-91052, Erlangen, Germany., Dudziak D; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Hartmannstraße 14, D-91052, Erlangen, Germany.; Medical Immunology Campus Erlangen (MICE), D-91054, Erlangen, Germany.; Deutsches Zentrum Immuntherapie (DZI), D-91054, Erlangen, Germany., Amon L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Hartmannstraße 14, D-91052, Erlangen, Germany., Hatscher L; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Hartmannstraße 14, D-91052, Erlangen, Germany., Kaszubowski T; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Hartmannstraße 14, D-91052, Erlangen, Germany., Lehmann CHK; Laboratory of Dendritic Cell Biology, Department of Dermatology, University Hospital Erlangen, Hartmannstraße 14, D-91052, Erlangen, Germany.; Medical Immunology Campus Erlangen (MICE), D-91054, Erlangen, Germany.
المصدر: European journal of immunology [Eur J Immunol] 2023 Dec; Vol. 53 (12), pp. e2249917. Date of Electronic Publication: 2022 Dec 23.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 1273201 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-4141 (Electronic) Linking ISSN: 00142980 NLM ISO Abbreviation: Eur J Immunol Subsets: MEDLINE
أسماء مطبوعة: Publication: <2005->: Weinheim : Wiley-VCH
Original Publication: Weinheim, Verlag Chemie GmbH.
مواضيع طبية MeSH: Thymus Gland* , Spleen*, Humans ; Flow Cytometry/methods ; Cell Separation/methods ; Dendritic Cells
مستخلص: This article is part of the Dendritic Cell Guidelines article series, which provides a collection of state-of-the-art protocols for the preparation, phenotype analysis by flow cytometry, generation, fluorescence microscopy, and functional characterization of mouse and human dendritic cells (DC) from lymphoid organs and various non-lymphoid tissues. Within this article, detailed protocols are presented that allow for the generation of single cell suspensions from human lymphohematopoietic tissues including blood, spleen, thymus, and tonsils with a focus on the subsequent analysis of DC via flow cytometry, as well as flow cytometric cell sorting of primary human DC. Further, prepared single cell suspensions as well as cell sorter-purified DC can be subjected to other applications including cellular enrichment procedures, RNA sequencing, functional assays, and many more. While all protocols were written by experienced scientists who routinely use them in their work, this article was also peer-reviewed by leading experts and approved by all co-authors, making it an essential resource for basic and clinical DC immunologists.
(© 2022 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.)
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معلومات مُعتمدة: CRC1181-TPA7 261193037 German Research Foundation; DU548/5-1 German Research Foundation; TRR305-TPB5 German Research Foundation; RTG2504-401821119 German Research Foundation; RTG2599 421758891 German Research Foundation; IZKF-A80 Interdisziplinäres Zentrum für klinische Forschung; IZKF-A87 Interdisziplinäres Zentrum für klinische Forschung; Bayresq.Net-IRIS Bavarian State Ministry of Science and Art; DU548/6-1 Agence Nationale de la Recherche
فهرسة مساهمة: Keywords: DC subpopulations; Dendritic cells; flow cytometry; human; lymphoid tissues; organ preparation
تواريخ الأحداث: Date Created: 20221223 Date Completed: 20231220 Latest Revision: 20231220
رمز التحديث: 20231220
DOI: 10.1002/eji.202249917
PMID: 36563130
قاعدة البيانات: MEDLINE
الوصف
تدمد:1521-4141
DOI:10.1002/eji.202249917