دورية أكاديمية
Autogenous dentin combined with mesenchymal stromal cells as an alternative alveolar bone graft: an in vivo study.
| العنوان: | Autogenous dentin combined with mesenchymal stromal cells as an alternative alveolar bone graft: an in vivo study. |
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| المؤلفون: | Barreiro BOB; School of Health and Life Sciences, Post-Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil., Koth VS; School of Health and Life Sciences, Post-Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil., Sesterheim P; Experimental Cardiology Center, Institute of Cardiology of Rio Grande do Sul, Porto Alegre, RS, Brazil., Salum FG; School of Health and Life Sciences, Post-Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil., Rübensam G; Toxicology and Pharmacology Research Center (INTOX), School of Health and Life Sciences, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil., Augustin AH; Institute of Petroleum and Natural Resources (IPR), Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil., Cherubini K; School of Health and Life Sciences, Post-Graduate Program in Dentistry, Pontifical Catholic University of Rio Grande do Sul, Porto Alegre, RS, Brazil. karen.cherubini@pucrs.br.; Serviço de Estomatologia, Hospital São Lucas PUCRS, Av. Ipiranga, 6690 Sala 231, Bairro Jardim Botânico, CEP: 90.610-000, Porto Alegre, RS, Brazil. karen.cherubini@pucrs.br. |
| المصدر: | Clinical oral investigations [Clin Oral Investig] 2023 May; Vol. 27 (5), pp. 1907-1922. Date of Electronic Publication: 2022 Dec 27. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 9707115 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1436-3771 (Electronic) Linking ISSN: 14326981 NLM ISO Abbreviation: Clin Oral Investig |
| أسماء مطبوعة: | Publication: Berlin : Springer-Verlag Original Publication: Berlin : Springer, c1997- |
| مواضيع طبية MeSH: | Alveolar Bone Grafting* , Mesenchymal Stem Cells* , Bone Substitutes*, Rats ; Animals ; Calcium ; X-Ray Microtomography ; Rats, Wistar ; Minerals ; Bone Regeneration ; Dentin ; Phosphorus |
| مستخلص: | Objective: Considering the chemical and structural properties of dentin, this study was aimed at evaluating the effect of dentin matrix alone or combined with mesenchymal stromal cells (MSC) on postextraction alveolar bone regeneration. Material and Methods: Wistar rats were subjected to tooth extraction with osteotomy and allocated into groups according to the graft inserted: (1) Gelita-Spon ® , (2) Bio-Oss ® , (3) Dentin, (4) MSC, (5) Dentin/MSC, and (6) Control. Maxillae were analyzed by means of hematoxylin and eosin (H&E) staining, immunohistochemical (IHC) analysis, microcomputed tomography (micro-CT), and scanning electron microscopy (SEM). Serum levels of calcium and phosphorus were quantified. Results: The Bio-Oss group showed less bone than Gelita-Spon and Dentin/MSC; no other significant differences were seen in H&E analysis. The Bio-Oss group showed higher expression of collagen type I compared to the Dentin and Dentin/MSC groups and also higher osteocalcin expression than the Dentin/MSC group. There was a tendency of higher expression of osteopontin in the MSC, Dentin, and Dentin/MSC groups and higher VEGF in the MSC group. On micro-CT analysis, the Bio-Oss and the Dentin/MSC groups exhibited greater bone volume than the Control. Serum calcium and phosphorus levels did not significantly differ between the groups. SEM analysis depicted particles of Bio-Oss and dentin in the respective groups, as well as significant cellularity in the MSC group. Conclusion: Autogenous nondemineralized dentin is an alternative for alveolar bone grafting, which can be improved by combination with MSC. Clinical Relevance: This work provides support for the clinical applicability of dentin graft alone or combined with MSC. (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.) |
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| فهرسة مساهمة: | Keywords: Alveolar ridge; Dentin; Graft; Mesenchymal stromal cells; Stem cells; Tooth |
| المشرفين على المادة: | 0 (Bio-Oss) SY7Q814VUP (Calcium) 0 (Minerals) 27YLU75U4W (Phosphorus) 0 (Bone Substitutes) |
| تواريخ الأحداث: | Date Created: 20221227 Date Completed: 20230508 Latest Revision: 20230508 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s00784-022-04840-z |
| PMID: | 36574044 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1436-3771 |
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| DOI: | 10.1007/s00784-022-04840-z |