دورية أكاديمية
Comparison of trace element concentrations in paired formalin-fixed paraffin-embedded and frozen human placentae.
| العنوان: | Comparison of trace element concentrations in paired formalin-fixed paraffin-embedded and frozen human placentae. |
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| المؤلفون: | Coyte RM; School of Earth Sciences, The Ohio State University, Columbus, OH, 43210, USA., Darrah TH; School of Earth Sciences, The Ohio State University, Columbus, OH, 43210, USA; Global Water Institute, The Ohio State University, Columbus, OH, 43210, USA., Barrett E; Department of Obstetrics and Gynecology, University of Rochester School of Medicine and Dentistry, Rochester, NY, 14642, USA; Department of Biostatistics and Epidemiology, Rutgers School of Public Health, Environmental and Occupational Health Sciences Institute, Piscataway, NJ, USA., O'Connor TG; Department of Obstetrics and Gynecology, University of Rochester School of Medicine and Dentistry, Rochester, NY, 14642, USA; Department of Psychiatry, University of Rochester, School of Medicine and Dentistry, Rochester, NY, 14642, USA; Department of Neuroscience, University of Rochester, School of Medicine and Dentistry, Rochester, NY, 14642, USA., Olesik JW; School of Earth Sciences, The Ohio State University, Columbus, OH, 43210, USA., Salafia CM; Placental Modulation Laboratory, Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA; Placental Analytics LLC, Larchmont, NY, USA., Shah R; Placental Modulation Laboratory, Institute for Basic Research in Developmental Disabilities, Staten Island, NY, USA; Placental Analytics LLC, Larchmont, NY, USA., Love T; Department of Biostatistics and Computational Biology, University of Rochester, Rochester, NY, 14627, USA., Miller RK; Department of Obstetrics and Gynecology, University of Rochester School of Medicine and Dentistry, Rochester, NY, 14642, USA; Departments of Pediatrics, Pathology and Environmental Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY, 14642, USA. Electronic address: R.K.Miller@Rochester.edu. |
| المصدر: | Placenta [Placenta] 2023 Jan; Vol. 131, pp. 98-103. Date of Electronic Publication: 2022 Dec 15. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 8006349 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-3102 (Electronic) Linking ISSN: 01434004 NLM ISO Abbreviation: Placenta Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2010- > : Amsterdam : Elsevier Original Publication: London W.B. Saunders. |
| مواضيع طبية MeSH: | Trace Elements*/analysis , Mercury*, Pregnancy ; Humans ; Female ; Cadmium ; Paraffin Embedding ; Lead ; Placenta/chemistry ; Manganese ; Chromium ; Formaldehyde |
| مستخلص: | Introduction: There is increasing interest in measuring metals concentrations in human placentas to better understand physiology, disease, and toxic and diagnostic exposures. For these purposes, formalin-fixed paraffin embedded (FFPE) tissues obtained at clinical pathology examination represent a valuable potential store of well-characterized tissues for analysis. However, the limited data that exist comparing metal concentrations in FFPE tissue to recently collected frozen tissues paints a confusing picture, and there is no published data directly comparing frozen and FFPE placental villus tissues. Methods: Paired samples of fresh frozen and FFPE tissue from 22 rapidly processed human singleton placentae were weighed and digested using standard clean laboratory procedures and subsequently analyzed for a suite of 13 metals using a PerkinElmer DRC II ICP-MS. The analytical results were compared using either a paired t-test or a sign test depending on data normality. Results: Concentrations of metals (aluminum (Al), arsenic (As), barium (Ba), cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), gadolinium (Gd), mercury (Hg), manganese (Mn), lead (Pb), strontium (Sr), and zinc (Zn)) measured in both types of tissue preparations (frozen and FFPE) displayed a consistent range with other studies and did not display significantly different values from each of the paired specimens for any of the 13 specific metals analyzed. Discussion: Within placentae, metals concentrations of measured trace, toxic and diagnostic elements (Al, As, Ba, Cd, Cr, Cu, Fe, Gd, Hg, Mn, Pb, Sr, and Zn) are consistent between FFPE and fresh placental villus tissue, without indications of systematic element loss or bias. FFPE from archived pathology specimens may offer an important and convenient alternative for measuring trace metals in human frozen placental tissues. Competing Interests: Declaration of competing interest All authors have no conflict of interests. (Copyright © 2022. Published by Elsevier Ltd.) |
| معلومات مُعتمدة: | R01 HD083369 United States HD NICHD NIH HHS; P30 ES001247 United States ES NIEHS NIH HHS; P30 ES005022 United States ES NIEHS NIH HHS; UH3 OD023271 United States OD NIH HHS; UH3 OD023349 United States OD NIH HHS; UG3 OD023271 United States OD NIH HHS |
| المشرفين على المادة: | 0 (Trace Elements) 00BH33GNGH (Cadmium) 2P299V784P (Lead) 42Z2K6ZL8P (Manganese) 0R0008Q3JB (Chromium) FXS1BY2PGL (Mercury) 1HG84L3525 (Formaldehyde) |
| تواريخ الأحداث: | Date Created: 20221228 Date Completed: 20230110 Latest Revision: 20230930 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.placenta.2022.12.003 |
| PMID: | 36577279 |
| قاعدة البيانات: | MEDLINE |
Full Text via ClinicalKey 
Full Text Finder | تدمد: | 1532-3102 |
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| DOI: | 10.1016/j.placenta.2022.12.003 |