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أعرض في EDS

Differential Sodium Current Remodelling Identifies Distinct Cellular Proarrhythmic Mechanisms in Paroxysmal vs Persistent Atrial Fibrillation.

التفاصيل البيبلوغرافية
العنوان: Differential Sodium Current Remodelling Identifies Distinct Cellular Proarrhythmic Mechanisms in Paroxysmal vs Persistent Atrial Fibrillation.
المؤلفون: Casini S; Amsterdam UMC, location University of Amsterdam, Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands. Electronic address: s.casini@amsterdamumc.nl., Marchal GA; Amsterdam UMC, location University of Amsterdam, Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands., Kawasaki M; Amsterdam UMC, location University of Amsterdam, Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands., Fabrizi B; Amsterdam UMC, location University of Amsterdam, Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands., Wesselink R; Amsterdam UMC, location University of Amsterdam, Department of Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands., Nariswari FA; Amsterdam UMC, location University of Amsterdam, Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands., Neefs J; Amsterdam UMC, location University of Amsterdam, Department of Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands., van den Berg NWE; Amsterdam UMC, location University of Amsterdam, Department of Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands., Driessen AHG; Amsterdam UMC, location University of Amsterdam, Department of Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands., de Groot JR; Amsterdam UMC, location University of Amsterdam, Department of Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands., Verkerk AO; Amsterdam UMC, location University of Amsterdam, Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands; Amsterdam UMC, location University of Amsterdam, Department of Medical Biology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands., Remme CA; Amsterdam UMC, location University of Amsterdam, Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Heart failure & Arrhythmias, Amsterdam, The Netherlands. Electronic address: s.casini@amsterdamumc.nl.
المصدر: The Canadian journal of cardiology [Can J Cardiol] 2023 Mar; Vol. 39 (3), pp. 277-288. Date of Electronic Publication: 2022 Dec 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 8510280 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1916-7075 (Electronic) Linking ISSN: 0828282X NLM ISO Abbreviation: Can J Cardiol Subsets: MEDLINE
أسماء مطبوعة: Publication: Jan./Feb. 2011- : Oxford, UK : Elsevier
Original Publication: [Oakville, Ont. : Canadian Cardiology Publications Inc., c1984-
مواضيع طبية MeSH: Atrial Fibrillation* , Atrial Appendage*, Humans ; Sodium ; Myocytes, Cardiac/metabolism ; Sodium Channels
مستخلص: Background: The cellular mechanisms underlying progression from paroxysmal to persistent atrial fibrillation (AF) are not fully understood, but alterations in (late) sodium current (I Na ) have been proposed. Human studies investigating electrophysiological changes at the paroxysmal stage of AF are sparse, with the majority employing right atrial appendage cardiomyocytes (CMs). We here investigated action potential (AP) characteristics and (late) I Na remodelling in left atrial appendage CMs (LAA-CMs) from patients with paroxysmal and persistent AF and patients in sinus rhythm (SR), as well as the potential contribution of the "neuronal" sodium channel SCN10A/Na V 1.8.
Methods: Peak I Na , late I Na and AP properties were investigated through patch-clamp analysis on single LAA-CMs, whereas quantitative polymerase chain reaction was used to assess SCN5A/SCN10A expression levels in LAA tissue.
Results: In paroxysmal and persistent AF LAA-CMs, AP duration was shorter than in SR LAA-CMs. Compared with SR, peak I Na and SCN5A expression were significantly decreased in paroxysmal AF, whereas they were restored to SR levels in persistent AF. Conversely, although late I Na was unchanged in paroxysmal AF compared with SR, it was significantly increased in persistent AF. Peak or late Na v 1.8-based I Na was not detected in persistent AF LAA-CMs. Similarly, expression of SCN10A was not observed in LAAs at any stage.
Conclusions: Our findings demonstrate differences in (late) I Na remodeling in LAA-CMs from patients with paroxysmal vs persistent AF, indicating distinct cellular proarrhythmic mechanisms in different AF forms. These observations are of particular relevance when considering potential pharmacologic approaches targeting (late) I Na in AF.
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
التعليقات: Comment in: Can J Cardiol. 2023 Mar;39(3):289-291. (PMID: 36586482)
المشرفين على المادة: 9NEZ333N27 (Sodium)
0 (Sodium Channels)
تواريخ الأحداث: Date Created: 20221231 Date Completed: 20230307 Latest Revision: 20230317
رمز التحديث: 20240628
DOI: 10.1016/j.cjca.2022.12.023
PMID: 36586483
قاعدة البيانات: MEDLINE
الوصف
تدمد:1916-7075
DOI:10.1016/j.cjca.2022.12.023