دورية أكاديمية
Single-nuclei transcriptome analysis of Huntington disease iPSC and mouse astrocytes implicates maturation and functional deficits.
العنوان: | Single-nuclei transcriptome analysis of Huntington disease iPSC and mouse astrocytes implicates maturation and functional deficits. |
---|---|
المؤلفون: | Reyes-Ortiz AM; Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92617, USA., Abud EM; Department of Neurobiology & Behavior, University of California, Irvine, Irvine, CA 92617, USA., Burns MS; Department of Neurobiology & Behavior, University of California, Irvine, Irvine, CA 92617, USA., Wu J; Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92617, USA., Hernandez SJ; Department of Neurobiology & Behavior, University of California, Irvine, Irvine, CA 92617, USA., McClure N; Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, Irvine, CA 92617, USA., Wang KQ; Department of Neurobiology & Behavior, University of California, Irvine, Irvine, CA 92617, USA., Schulz CJ; Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, Irvine, CA 92617, USA., Miramontes R; Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, Irvine, CA 92617, USA., Lau A; Department of Psychiatry & Human Behavior, University of California, Irvine, Irvine, CA 92617, USA., Michael N; Department of Neurobiology & Behavior, University of California, Irvine, Irvine, CA 92617, USA., Miyoshi E; Department of Neurobiology & Behavior, University of California, Irvine, Irvine, CA 92617, USA., Van Vactor D; Harvard Medical School, Department of Cell Biology, Boston, MA 02115, USA., Reidling JC; Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, Irvine, CA 92617, USA., Blurton-Jones M; Department of Neurobiology & Behavior, University of California, Irvine, Irvine, CA 92617, USA.; Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, Irvine, CA 92617, USA.; Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, Irvine, CA 92617, USA., Swarup V; Department of Neurobiology & Behavior, University of California, Irvine, Irvine, CA 92617, USA.; Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, Irvine, CA 92617, USA., Poon WW; Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, Irvine, CA 92617, USA., Lim RG; Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, Irvine, CA 92617, USA., Thompson LM; Department of Biological Chemistry, University of California, Irvine, Irvine, CA 92617, USA.; Department of Neurobiology & Behavior, University of California, Irvine, Irvine, CA 92617, USA.; Sue and Bill Gross Stem Cell Research Center, University of California, Irvine, Irvine, CA 92617, USA.; Department of Psychiatry & Human Behavior, University of California, Irvine, Irvine, CA 92617, USA.; Institute for Memory Impairments and Neurological Disorders, University of California, Irvine, Irvine, CA 92617, USA. |
المصدر: | IScience [iScience] 2022 Dec 06; Vol. 26 (1), pp. 105732. Date of Electronic Publication: 2022 Dec 06 (Print Publication: 2023). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Cell Press Country of Publication: United States NLM ID: 101724038 Publication Model: eCollection Cited Medium: Internet ISSN: 2589-0042 (Electronic) Linking ISSN: 25890042 NLM ISO Abbreviation: iScience Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: [Cambridge, MA] : Cell Press, [2018]- |
مستخلص: | Huntington disease (HD) is a neurodegenerative disorder caused by expanded CAG repeats in the huntingtin gene that alters cellular homeostasis, particularly in the striatum and cortex. Astrocyte signaling that establishes and maintains neuronal functions are often altered under pathological conditions. We performed single-nuclei RNA-sequencing on human HD patient-induced pluripotent stem cell (iPSC)-derived astrocytes and on striatal and cortical tissue from R6/2 HD mice to investigate high-resolution HD astrocyte cell state transitions. We observed altered maturation and glutamate signaling in HD human and mouse astrocytes. Human HD astrocytes also showed upregulated actin-mediated signaling, suggesting that some states may be cell-autonomous and human specific. In both species, astrogliogenesis transcription factors may drive HD astrocyte maturation deficits, which are supported by rescued climbing deficits in HD drosophila with NFIA knockdown. Thus, dysregulated HD astrocyte states may induce dysfunctional astrocytic properties, in part due to maturation deficits influenced by astrogliogenesis transcription factor dysregulation. Competing Interests: The authors declare no competing interests. Some authors have additional affiliations as noted below: Andrea M. Reyes-Ortiz: Genentech, Inc., South San Francisco, California. Edsel M. Abud: Scripps Research Translational Institute, La Jolla, California and Division of Allergy, Asthma, and Immunology, Scripps Clinic, San Diego, California. Ryan G. Lim: Modulo Bio, Inc., San Diego, California. Wayne W. Poon: NeuCyte, Inc., Mountain View, California. Patent Pending: GENERATION AND APPLICATION OF FUNCTIONAL HUMAN ASTROCYTES FROM PLURIPOTENT STEM CELLS. Inventors: Edsel Abud, Wayne Poon. (© 2022 The Authors.) |
References: | Acta Neuropathol Commun. 2020 Feb 18;8(1):19. (PMID: 32070434) Nat Commun. 2022 Dec 21;13(1):7791. (PMID: 36543778) Neural Regen Res. 2021 Sep;16(9):1702-1710. (PMID: 33510058) Mol Neurobiol. 2017 Mar;54(2):1577-1586. (PMID: 27221610) Elife. 2021 Apr 19;10:. (PMID: 33871358) Cell Rep. 2021 Jul 6;36(1):109308. (PMID: 34233199) Acta Neuropathol. 2010 Jan;119(1):7-35. (PMID: 20012068) Cell Stem Cell. 2012 Aug 3;11(2):264-78. (PMID: 22748968) Hum Mol Genet. 2010 Aug 1;19(15):3053-67. (PMID: 20494921) Matrix Biol Plus. 2021 Oct 23;12:100089. (PMID: 34786551) Mol Cell. 2009 Mar 27;33(6):717-26. (PMID: 19217333) Cell Stem Cell. 2018 Oct 4;23(4):557-571.e8. (PMID: 30290178) Neurobiol Dis. 2013 Oct;58:192-9. (PMID: 23756199) Nat Neurosci. 2015 Jul;18(7):942-52. (PMID: 26108722) Front Mol Neurosci. 2021 Mar 31;14:654031. (PMID: 33867936) Hum Mol Genet. 2018 Dec 15;27(24):4303-4314. (PMID: 30239724) Hum Mol Genet. 2002 Aug 15;11(17):1911-26. (PMID: 12165554) Nat Neurosci. 2016 Apr;19(4):623-33. (PMID: 26900923) Acta Neuropathol Commun. 2014 Dec 20;2:179. (PMID: 25526803) J Neurosci. 2014 Sep 3;34(36):11929-47. (PMID: 25186741) Neuron. 1996 Mar;16(3):675-86. (PMID: 8785064) BMC Bioinformatics. 2013 Apr 15;14:128. (PMID: 23586463) Mol Cell. 2004 Sep 24;15(6):853-65. (PMID: 15383276) Arch Neurol. 1986 Oct;43(10):1058-63. (PMID: 2428340) Cell. 2015 Nov 19;163(5):1064-1078. (PMID: 26590417) J Neurosci. 2016 Mar 23;36(12):3453-70. (PMID: 27013675) J Neurosci Methods. 2008 Jun 30;171(2):239-47. (PMID: 18471889) Genome Res. 2015 Oct;25(10):1491-8. (PMID: 26430159) Hum Mol Genet. 2015 Jun 1;24(11):3257-71. (PMID: 25740845) J Neuropathol Exp Neurol. 2001 Feb;60(2):161-72. (PMID: 11273004) Nat Neurosci. 2017 May;20(5):648-660. (PMID: 28319609) Proc Natl Acad Sci U S A. 2009 Dec 29;106(52):22480-5. (PMID: 20018729) Cell. 1996 Nov 1;87(3):493-506. (PMID: 8898202) Cold Spring Harb Protoc. 2012 Jan 01;2012(1):1-17. (PMID: 22194269) Hum Mol Genet. 2006 Mar 15;15(6):965-77. (PMID: 16467349) Neurobiol Dis. 2013 Feb;50:160-70. (PMID: 23089356) Nat Rev Neurosci. 2006 Jan;7(1):41-53. (PMID: 16371949) Neuron. 1994 Sep;13(3):713-25. (PMID: 7917301) BMC Bioinformatics. 2011 Aug 31;12:357. (PMID: 21880147) Neuron. 2012 Apr 12;74(1):79-94. (PMID: 22500632) J Neuropathol Exp Neurol. 1991 Nov;50(6):729-42. (PMID: 1836225) J Neurosci. 2009 Mar 11;29(10):3276-87. (PMID: 19279265) Trends Neurosci. 2006 Feb;29(2):82-90. (PMID: 16377000) Lancet Neurol. 2011 Jan;10(1):83-98. (PMID: 21163446) Neurobiol Dis. 2008 Jun;30(3):365-374. (PMID: 18417352) Proc Natl Acad Sci U S A. 2014 Nov 25;111(47):16889-94. (PMID: 25385587) Acta Neuropathol. 2021 Mar;141(3):399-413. (PMID: 33517535) Cell Metab. 2019 Jun 4;29(6):1258-1273.e11. (PMID: 30930170) Neuron. 2016 Jan 6;89(1):37-53. (PMID: 26687838) Hum Mol Genet. 2012 Dec 15;21(24):5280-93. (PMID: 22965876) Nat Neurosci. 2014 May;17(5):694-703. (PMID: 24686787) Trends Biochem Sci. 2003 Aug;28(8):425-33. (PMID: 12932731) Cell Syst. 2018 Jul 25;7(1):28-40.e4. (PMID: 29936182) Proc Natl Acad Sci U S A. 2005 Mar 8;102(10):3777-81. (PMID: 15716359) Ment Retard Dev Disabil Res Rev. 2001;7(3):153-7. (PMID: 11553930) Sci Transl Med. 2019 Oct 16;11(514):. (PMID: 31619545) Stem Cell Reports. 2015 Dec 8;5(6):1023-1038. (PMID: 26651603) Nat Genet. 1998 Oct;20(2):198-202. (PMID: 9771716) J Neurosci. 1995 Mar;15(3 Pt 1):1835-53. (PMID: 7891138) J Nucl Med. 2006 Feb;47(2):215-22. (PMID: 16455626) Neuron. 2010 Jan 28;65(2):178-90. (PMID: 20152125) Hum Mol Genet. 2015 Mar 1;24(5):1441-56. (PMID: 25480889) Hum Mol Genet. 2010 Feb 15;19(4):573-83. (PMID: 19933700) ACS Chem Neurosci. 2014 Jul 16;5(7):494-6. (PMID: 24842291) Neurosci Lett. 1986 Jun 18;67(2):198-202. (PMID: 2873534) J Neurosci. 2003 Mar 15;23(6):2193-202. (PMID: 12657678) Cell Stem Cell. 2019 Jan 3;24(1):107-122.e7. (PMID: 30554964) Physiol Rev. 2010 Jul;90(3):905-81. (PMID: 20664076) Ann Neurol. 2015 Aug;78(2):178-92. (PMID: 25914140) Front Neurosci. 2019 Jun 28;13:669. (PMID: 31316341) Sci Rep. 2018 Jul 27;8(1):11355. (PMID: 30054496) Cell. 2019 Jun 13;177(7):1888-1902.e21. (PMID: 31178118) NPJ Schizophr. 2019 Jan 29;5(1):3. (PMID: 30696826) Science. 2013 Jan 11;339(6116):197-200. (PMID: 23307741) Front Cell Neurosci. 2015 Aug 03;9:278. (PMID: 26283915) Hum Mol Genet. 2001 Jan 15;10(2):137-44. (PMID: 11152661) Proc Natl Acad Sci U S A. 2019 May 7;116(19):9622-9627. (PMID: 31015293) Nucleic Acids Res. 2016 Jul 8;44(W1):W90-7. (PMID: 27141961) Cell. 1993 Mar 26;72(6):971-83. (PMID: 8458085) Cell Rep. 2017 May 16;19(7):1365-1377. (PMID: 28514657) Stem Cell Reports. 2020 Mar 10;14(3):406-419. (PMID: 32109367) Neuron. 1988 Oct;1(8):623-34. (PMID: 2908446) Nat Neurosci. 2012 Jun 17;15(7):979-87. (PMID: 22706267) Brain Res Bull. 2016 Jul;125:1-18. (PMID: 27021168) Nat Rev Dis Primers. 2015 Apr 23;1:15005. (PMID: 27188817) Nat Methods. 2014 Oct;11(10):982-4. (PMID: 25264773) Mol Biol Cell. 2014 Jan;25(1):169-83. (PMID: 24173718) Mol Brain. 2012 May 21;5:17. (PMID: 22613578) Brain Res. 1982 Aug 5;245(1):117-25. (PMID: 6214299) Trends Neurosci. 2017 Jul;40(7):422-437. (PMID: 28578789) J Anat. 1953 Oct;87(4):387-406. (PMID: 13117757) Nat Methods. 2021 Apr;18(4):374-377. (PMID: 33795878) Genome Biol. 2006;7(10):R100. (PMID: 17076895) Science. 2020 Aug 14;369(6505):787-793. (PMID: 32675289) |
معلومات مُعتمدة: | S10 OD021718 United States OD NIH HHS; R35 NS116872 United States NS NINDS NIH HHS; S10 RR025496 United States RR NCRR NIH HHS; R21 NS123207 United States NS NINDS NIH HHS; R01 NS089076 United States NS NINDS NIH HHS; RF1 AG071683 United States AG NIA NIH HHS; T32 NS082174 United States NS NINDS NIH HHS; S10 OD010794 United States OD NIH HHS; P01 NS092525 United States NS NINDS NIH HHS; P30 CA062203 United States CA NCI NIH HHS; R01 AG071683 United States AG NIA NIH HHS |
فهرسة مساهمة: | Keywords: Neurology; Omics |
تواريخ الأحداث: | Date Created: 20230102 Latest Revision: 20240321 |
رمز التحديث: | 20240321 |
مُعرف محوري في PubMed: | PMC9800269 |
DOI: | 10.1016/j.isci.2022.105732 |
PMID: | 36590162 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2589-0042 |
---|---|
DOI: | 10.1016/j.isci.2022.105732 |