دورية أكاديمية
أعرض في EDS

In vitro assessment of PEG-6000 coated-ZnO nanoparticles: modulating action to the resisted antibiotic activity against APEC.

التفاصيل البيبلوغرافية
العنوان: In vitro assessment of PEG-6000 coated-ZnO nanoparticles: modulating action to the resisted antibiotic activity against APEC.
المؤلفون: Badry A; Veterinarian, Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt., Ibrahim AAEH; Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt., Said MI; Department of Chemistry, Faculty of Science, Assiut University, Asyut, Egypt., Nasr AAE; Poultry Diseases Department, Agriculture Research Center, Animal Health Research Institute, Assiut Lab. Egypt, Asyut, Egypt., Mohamed MA; Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt., Hassan AK; Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt., Safwat MM; Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt. marwa.mohammed@vet.aun.edu.eg.
المصدر: BMC veterinary research [BMC Vet Res] 2023 Jan 04; Vol. 19 (1), pp. 1. Date of Electronic Publication: 2023 Jan 04.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, 2005-
مواضيع طبية MeSH: Escherichia coli* , Zinc Oxide*/pharmacology , Zinc Oxide*/chemistry, Animals ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Microbial Sensitivity Tests/veterinary ; Birds ; Polyethylene Glycols/pharmacology ; Streptomycin/pharmacology ; Surface-Active Agents/pharmacology
مستخلص: Background: Avian pathogenic Escherichia coli (APEC) are considered a growing health problem to both poultry and the public, particularly due to its multi-drug resistance. Zinc oxide nanoparticles (ZnO-NPs) are a promising multi-benefit candidate. This study focused on boosting the antimicrobial effect of the chemically synthesized ZnO-NPs using Polyethylene glycol-6000 (PEG-6000) and evaluating their potential to recover the sensitivity of Florfenicol and Streptomycin-resistant APEC to these drugs in a concentration range of 0.1-0.4 mg/mL. Four samples of ZnO-NPs were formulated and tested microbiologically.
Results: The physicochemical characterization showed well-crystallized spherical in situ synthesized ZnO-NPs using PEG-6000 (surfactant) and ethanol (co-surfactant) of ∼19-67 nm particle size after coating with PEG-6000 molecules. These ZnO-NPs demonstrated a strong concentration-dependent antibacterial effect against multidrug-resistant APEC strains, with a minimum inhibitory concentration of 0.1 mg/mL, Combining PEG-6000 coated in situ synthesized ZnO-NPs and Florfenicol induced 60% high sensitivity (30 mm inhibitory-zone), 30% intermediate sensitivity, and 10% resistance against APEC strains. The combination with Streptomycin revealed 50% high sensitivity, 30% intermediate sensitivity, and 20% resistance with a 20 mm maximum zone of inhibition using agar well diffusion test.
Conclusion: In situ preparation of ZnO-NPs using PEG-6000 and ethanol followed by coating with PEG-6000 enhanced its antibacterial activity in minimum inhibitory concentration and regained the efficacy of Florfenicol and Streptomycin against APEC, referring to a non-antibiotic antimicrobial alternative and an effective combination regimen against multidrug-resistant APEC E. coli in veterinary medicine.
(© 2023. The Author(s).)
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فهرسة مساهمة: Keywords: APEC; FICI; Florfenicol; MIC; PEG-6000 coated ZnO nanoparticles; Resistance; Streptomycin
المشرفين على المادة: 0 (Anti-Bacterial Agents)
SOI2LOH54Z (Zinc Oxide)
9J97307Y1H (florfenicol)
30IQX730WE (Polyethylene Glycol 6000)
3WJQ0SDW1A (Polyethylene Glycols)
Y45QSO73OB (Streptomycin)
0 (Surface-Active Agents)
تواريخ الأحداث: Date Created: 20230103 Date Completed: 20230105 Latest Revision: 20230111
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC9811692
DOI: 10.1186/s12917-022-03562-4
PMID: 36597079
قاعدة البيانات: MEDLINE
الوصف
تدمد:1746-6148
DOI:10.1186/s12917-022-03562-4