دورية أكاديمية
In vitro assessment of PEG-6000 coated-ZnO nanoparticles: modulating action to the resisted antibiotic activity against APEC.
العنوان: | In vitro assessment of PEG-6000 coated-ZnO nanoparticles: modulating action to the resisted antibiotic activity against APEC. |
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المؤلفون: | Badry A; Veterinarian, Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt., Ibrahim AAEH; Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt., Said MI; Department of Chemistry, Faculty of Science, Assiut University, Asyut, Egypt., Nasr AAE; Poultry Diseases Department, Agriculture Research Center, Animal Health Research Institute, Assiut Lab. Egypt, Asyut, Egypt., Mohamed MA; Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt., Hassan AK; Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt., Safwat MM; Avian and Rabbit Medicine Department, Faculty of Veterinary Medicine, Assiut University, Asyut, Egypt. marwa.mohammed@vet.aun.edu.eg. |
المصدر: | BMC veterinary research [BMC Vet Res] 2023 Jan 04; Vol. 19 (1), pp. 1. Date of Electronic Publication: 2023 Jan 04. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101249759 Publication Model: Electronic Cited Medium: Internet ISSN: 1746-6148 (Electronic) Linking ISSN: 17466148 NLM ISO Abbreviation: BMC Vet Res Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : BioMed Central, 2005- |
مواضيع طبية MeSH: | Escherichia coli* , Zinc Oxide*/pharmacology , Zinc Oxide*/chemistry, Animals ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Microbial Sensitivity Tests/veterinary ; Birds ; Polyethylene Glycols/pharmacology ; Streptomycin/pharmacology ; Surface-Active Agents/pharmacology |
مستخلص: | Background: Avian pathogenic Escherichia coli (APEC) are considered a growing health problem to both poultry and the public, particularly due to its multi-drug resistance. Zinc oxide nanoparticles (ZnO-NPs) are a promising multi-benefit candidate. This study focused on boosting the antimicrobial effect of the chemically synthesized ZnO-NPs using Polyethylene glycol-6000 (PEG-6000) and evaluating their potential to recover the sensitivity of Florfenicol and Streptomycin-resistant APEC to these drugs in a concentration range of 0.1-0.4 mg/mL. Four samples of ZnO-NPs were formulated and tested microbiologically. Results: The physicochemical characterization showed well-crystallized spherical in situ synthesized ZnO-NPs using PEG-6000 (surfactant) and ethanol (co-surfactant) of ∼19-67 nm particle size after coating with PEG-6000 molecules. These ZnO-NPs demonstrated a strong concentration-dependent antibacterial effect against multidrug-resistant APEC strains, with a minimum inhibitory concentration of 0.1 mg/mL, Combining PEG-6000 coated in situ synthesized ZnO-NPs and Florfenicol induced 60% high sensitivity (30 mm inhibitory-zone), 30% intermediate sensitivity, and 10% resistance against APEC strains. The combination with Streptomycin revealed 50% high sensitivity, 30% intermediate sensitivity, and 20% resistance with a 20 mm maximum zone of inhibition using agar well diffusion test. Conclusion: In situ preparation of ZnO-NPs using PEG-6000 and ethanol followed by coating with PEG-6000 enhanced its antibacterial activity in minimum inhibitory concentration and regained the efficacy of Florfenicol and Streptomycin against APEC, referring to a non-antibiotic antimicrobial alternative and an effective combination regimen against multidrug-resistant APEC E. coli in veterinary medicine. (© 2023. The Author(s).) |
References: | Lett Appl Microbiol. 2004;38(3):197-205. (PMID: 14962040) Nanomaterials (Basel). 2020 Mar 15;10(3):. (PMID: 32183496) Biomater Investig Dent. 2020 Jul 23;7(1):105-109. (PMID: 32939454) Int J Nanomedicine. 2019 Dec 02;14:9395-9410. (PMID: 31819439) Nanoscale. 2018 Mar 8;10(10):4927-4939. (PMID: 29480295) Pathog Glob Health. 2015;109(7):309-18. (PMID: 26343252) Int J Med Microbiol. 2013 Dec;303(8):475-83. (PMID: 23891276) J Clin Microbiol. 2004 Aug;42(8):3483-9. (PMID: 15297487) Antibiotics (Basel). 2020 Apr 16;9(4):. (PMID: 32316130) Vet Microbiol. 2005 Oct 31;110(3-4):245-53. (PMID: 16150559) Bioinorg Chem Appl. 2015;2015:536854. (PMID: 26451136) ACS Nano. 2013 Nov 26;7(11):9518-25. (PMID: 24274814) Appl Phys Lett. 2007 May 24;90(213902):2139021-2139023. (PMID: 18160973) Vet J. 2016 Mar;209:174-9. (PMID: 26832810) Nanomedicine (Lond). 2018 Nov;13(21):2675-2690. (PMID: 30346253) Langmuir. 2015 Aug 25;31(33):9155-62. (PMID: 26222950) |
فهرسة مساهمة: | Keywords: APEC; FICI; Florfenicol; MIC; PEG-6000 coated ZnO nanoparticles; Resistance; Streptomycin |
المشرفين على المادة: | 0 (Anti-Bacterial Agents) SOI2LOH54Z (Zinc Oxide) 9J97307Y1H (florfenicol) 30IQX730WE (Polyethylene Glycol 6000) 3WJQ0SDW1A (Polyethylene Glycols) Y45QSO73OB (Streptomycin) 0 (Surface-Active Agents) |
تواريخ الأحداث: | Date Created: 20230103 Date Completed: 20230105 Latest Revision: 20230111 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC9811692 |
DOI: | 10.1186/s12917-022-03562-4 |
PMID: | 36597079 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1746-6148 |
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DOI: | 10.1186/s12917-022-03562-4 |