دورية أكاديمية
Evaluation of a rapid susceptibility test of polymyxin B by MALDI-TOF.
العنوان: | Evaluation of a rapid susceptibility test of polymyxin B by MALDI-TOF. |
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المؤلفون: | Barth PO; LABRESIS - Laboratório de Pesquisa em Resistência Bacteriana, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; PPGCM - Programa de Pós-Graduação em Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Volpato FCZ; LABRESIS - Laboratório de Pesquisa em Resistência Bacteriana, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; PPGCF - Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Moreira NK; LABRESIS - Laboratório de Pesquisa em Resistência Bacteriana, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; PPGCF - Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Wink PL; LABRESIS - Laboratório de Pesquisa em Resistência Bacteriana, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; PPGCF - Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., de Souza ÂC; PPGCM - Programa de Pós-Graduação em Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil., Barth AL; LABRESIS - Laboratório de Pesquisa em Resistência Bacteriana, Hospital de Clínicas de Porto Alegre, Porto Alegre, Brazil.; PPGCM - Programa de Pós-Graduação em Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.; PPGCF - Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. |
المصدر: | Frontiers in microbiology [Front Microbiol] 2022 Dec 19; Vol. 13, pp. 1075650. Date of Electronic Publication: 2022 Dec 19 (Print Publication: 2022). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Lausanne : Frontiers Research Foundation |
مستخلص: | Introduction: Infections caused by multidrug-resistant microorganisms have become increasingly common in hospital environments around the world. Gram-negative bacilli stands out among multidrug-resistant bacteria mostly due to the production of carbapenemase enzymes which lead to resistance to most β-lactam antibiotics including the carbapenems. As a consequence, polymyxins have been reintroduced in the clinic as a last resort to treat infections caused by Gram-negative bacilli resistant to carbapenems. However, the only reliable method to evaluate the susceptibility to polymyxins is the broth microdilution, a laborious and time-consuming technique. Among infections caused by multidrug-resistant bacteria, bloodstream infections are the most worrisome as they can lead to sepsis and septic shock with high mortality rates. Objective: Considering the severity of sepsis and the need for a treatment guided for the susceptibility test in vitro , this work aimed to evaluate a rapid method of polymyxins susceptibility either from colonies grown on agar or directly from positive blood culture bottles using the technology of MALDI-TOF. Methods: The method was based on the "direct on target microdroplets growth assay" (DOT-MGA) originally developed by Idelevich and collaborators with some modifications (Adapted DOT-MGA). Isolates of Enterobacterales and non-fermenting Gram-negative bacilli resistant to carbapenems were obtained from patients attending a tertiary care hospital in southern Brazil and tested as follows: 122 isolates from colonies grown on agar plates and 117 isolates directly from spiked positive blood cultures. Results: The adapted DOT-MGA presented 95 and 100% of categorical agreement considering the colonies grown on agar plates and directly from positive blood cultures, respectively. Discussion: The adapted DOT-MGA test proved to be a reliable technique to evaluate the susceptibility to polymyxins to be used in microbiology laboratories with the MALDI-TOF equipment. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Barth, Volpato, Moreira, Wink, de Souza and Barth.) |
References: | Diagnostics (Basel). 2021 Sep 29;11(10):. (PMID: 34679499) Front Microbiol. 2018 Aug 06;9:1744. (PMID: 30127772) Clin Microbiol Infect. 2010 Feb;16(2):112-22. (PMID: 20085605) J Clin Microbiol. 2021 Nov 18;59(12):e0181419. (PMID: 34133892) Clin Microbiol Infect. 2018 Jul;24(7):738-743. (PMID: 29079147) Lancet Infect Dis. 2019 Jun;19(6):561-562. (PMID: 31047851) J Glob Antimicrob Resist. 2018 Mar;12:124-136. (PMID: 28962863) J Clin Microbiol. 2016 Sep;54(9):2273-7. (PMID: 27307457) Crit Care Med. 2006 Jun;34(6):1589-96. (PMID: 16625125) Lancet Infect Dis. 2018 Mar;18(3):318-327. (PMID: 29276051) Sci Rep. 2017 Jul 11;7(1):5071. (PMID: 28698568) Chest. 2009 Nov;136(5):1237-1248. (PMID: 19696123) Front Cell Infect Microbiol. 2022 Mar 15;12:823684. (PMID: 35372099) Clin Microbiol Rev. 2012 Oct;25(4):682-707. (PMID: 23034326) Front Med (Lausanne). 2019 Apr 16;6:74. (PMID: 31041313) mSphere. 2019 Apr 10;4(2):. (PMID: 30971443) Braz J Infect Dis. 2022 Nov 30;27(1):102721. (PMID: 36462577) |
فهرسة مساهمة: | Keywords: Gram-negative bacilli; MALDI-TOF; blood cultures; polymyxin B; sepsis |
تواريخ الأحداث: | Date Created: 20230105 Latest Revision: 20230111 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC9806129 |
DOI: | 10.3389/fmicb.2022.1075650 |
PMID: | 36601408 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1664-302X |
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DOI: | 10.3389/fmicb.2022.1075650 |