دورية أكاديمية
Binding of Equine Seminal Lactoferrin/Superoxide Dismutase (SOD-3) Complex Is Biased towards Dead Spermatozoa.
العنوان: | Binding of Equine Seminal Lactoferrin/Superoxide Dismutase (SOD-3) Complex Is Biased towards Dead Spermatozoa. |
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المؤلفون: | Alghamdi AS; Departments of Agriculture and Natural Resources, University of Minnesota Crookston, Crookston, MN 56716, USA., Fedorka CE; Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA., Scoggin KE; Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA., Esteller-Vico A; Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA., Beatty K; Departments of Agriculture and Natural Resources, University of Minnesota Crookston, Crookston, MN 56716, USA., Davolli G; Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA., Ball BA; Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA., Troedsson MHT; Gluck Equine Research Center, University of Kentucky, Lexington, KY 40546, USA. |
المصدر: | Animals : an open access journal from MDPI [Animals (Basel)] 2022 Dec 23; Vol. 13 (1). Date of Electronic Publication: 2022 Dec 23. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Molecular Diversity Preservation International Country of Publication: Switzerland NLM ID: 101635614 Publication Model: Electronic Cited Medium: Print ISSN: 2076-2615 (Print) Linking ISSN: 20762615 NLM ISO Abbreviation: Animals (Basel) Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Basel, Switzerland : Molecular Diversity Preservation International, 2011- |
مستخلص: | Sperm-neutrophil binding is an important facet of breeding and significantly impacts fertility. While a specific seminal plasma protein has been found to reduce this binding and improve fertility (CRISP-3), additional molecule(s) appear to promote binding between defective sperm and neutrophils. Recent work has suggested one of these proteins is lactoferrin (LF), an 80 kDa iron-binding protein found throughout the body, but the purity of the protein was not confirmed. It is unknown if LF binds to sperm selectively based on viability, and if receptors for LF are located on equine sperm. To evaluate this, we attempted to purify equine seminal LF from five stallions (n = 5), biotinylate LF, and evaluate potential binding site(s) on spermatozoa. LF was consistently associated with superoxide dismutase (SOD-3), and all attempts to separate the two proteins were unsuccessful. Flow cytometric and microscopic analyses were used to compare LF/SOD-3 binding to viable and nonviable spermatozoa. Additionally, various methods of biotinylation were assessed to optimize this methodology. Biotinylation of seminal plasma protein was an effective and efficient method to study seminal plasma protein properties, and the binding site for LF/SOD-3 was found to be broadly localized to the entire sperm cell surface as well as selective towards nonviable/defective sperm. Although we were not able to determine if the binding to equine spermatozoa was through LF or SOD-3, we can conclude that equine seminal LF is tightly bound to SOD-3 and this protein complex binds selectively to nonviable spermatozoa, possibly to mark them for elimination by neutrophil phagocytosis. |
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فهرسة مساهمة: | Keywords: binding; equine; lactoferrin; seminal plasma; spermatozoa |
تواريخ الأحداث: | Date Created: 20230108 Latest Revision: 20230111 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC9817809 |
DOI: | 10.3390/ani13010052 |
PMID: | 36611662 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2076-2615 |
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DOI: | 10.3390/ani13010052 |