دورية أكاديمية
أعرض في EDS

UPLC-MS-ESI-QTOF Analysis and Antifungal Activity of Aqueous Extracts of Spondias tuberosa .

التفاصيل البيبلوغرافية
العنوان: UPLC-MS-ESI-QTOF Analysis and Antifungal Activity of Aqueous Extracts of Spondias tuberosa .
المؤلفون: Santos ATLD; Pimenta Campus, Regional University of Cariri-URCA, Crato 63105-010, Brazil., Carneiro JNP; Pimenta Campus, Regional University of Cariri-URCA, Crato 63105-010, Brazil., da Cruz RP; Pimenta Campus, Regional University of Cariri-URCA, Crato 63105-010, Brazil., Sales DL; Pimenta Campus, Regional University of Cariri-URCA, Crato 63105-010, Brazil., Andrade-Pinheiro JC; Brejo Santo Campus, Federal University of Cariri-UFCA, Brejo Santo 63260-000, Brazil., de Freitas MA; Department of Mycology, Federal University of Pernambuco-University City, Recife 50670-901, Brazil., Kerntopf MR; Pimenta Campus, Regional University of Cariri-URCA, Crato 63105-010, Brazil., Delmondes GA; Pimenta Campus, Regional University of Cariri-URCA, Crato 63105-010, Brazil., Ribeiro PRV; Embrapa Tropical Agroindustry, Fortaleza 60511-110, Brazil., de Brito ES; Embrapa Tropical Agroindustry, Fortaleza 60511-110, Brazil., Batista FLA; Pimenta Campus, Regional University of Cariri-URCA, Crato 63105-010, Brazil., Magalhães FEA; Tauá Campus, State University of Ceara, Tauá 63660-000, Brazil., Pita Neto IC; São José Campus, CECAPE-College of Dentistry, Juazeiro do Norte 63024-015, Brazil., Morais-Braga MFB; Pimenta Campus, Regional University of Cariri-URCA, Crato 63105-010, Brazil., Kowalski R; Department of Analysis and Food Quality Assessment, University of Life Sciences in Lublin, 8 Skromna Str., 20-704 Lublin, Poland., Kowalska G; Department of Tourism and Recreation, University of Life Sciences in Lublin, 15 Akademicka Str., 20-950 Lublin, Poland., Szopa A; Department of Clinical Pharmacy and Pharmaceutical Care, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland., Baj T; Department of Pharmacognosy with the Medicinal Plant Garden, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland., Coutinho HDM; Pimenta Campus, Regional University of Cariri-URCA, Crato 63105-010, Brazil.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2022 Dec 30; Vol. 28 (1). Date of Electronic Publication: 2022 Dec 30.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Antifungal Agents*/chemistry , Fluconazole*/pharmacology, Chromatography, Liquid ; Chromatography, High Pressure Liquid ; Plant Extracts/chemistry ; Tandem Mass Spectrometry ; Candida albicans ; Candida tropicalis ; Microbial Sensitivity Tests
مستخلص: This study aimed to identify the chemical composition of the Spondias tuberosa aqueous leaf and root extracts (EALST and EARST) and to evaluate their effect, comparatively, against opportunistic pathogenic fungi. Ultra-Performance Liquid Chromatography Coupled to a Quadrupole/Time of Flight System (UPLC-MS-ESI-QTOF) was employed for chemical analysis. Candida albicans and C. tropicalis standard strains and clinical isolates were used (CA INCQS 40006, CT INCQS 40042, CA URM 5974, and CT URM 4262). The 50% Inhibitory Concentration for the fungal population (IC 50 ) was determined for both the intrinsic action of the extracts and the extract/fluconazole (FCZ) associations. The determination of the Minimum Fungicidal Concentration (MFC) and the verification of effects over fungal morphological transitions were performed by subculture in Petri dishes and humid chambers, respectively, both based on micro-dilution. UPLC-MS-ESI-QTOF analysis revealed the presence of phenolic and flavonoid compounds. The association of the extracts with fluconazole, resulted in IC 50 values from 2.62 µg/mL to 308.96 µg/mL. The MFC of the extracts was ≥16,384 µg/mL for all tested strains, while fluconazole obtained an MFC of 8192 µg/mL against C. albicans strains. A reduction in MFC against CA URM 5974 (EALST: 2048 µg/mL and EARST: 1024 µg/mL) occurred in the extract/fluconazole association.
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فهرسة مساهمة: Keywords: flavonoids; fluconazole; popular medicine; umbu
المشرفين على المادة: 0 (Antifungal Agents)
8VZV102JFY (Fluconazole)
0 (Plant Extracts)
تواريخ الأحداث: Date Created: 20230108 Date Completed: 20230110 Latest Revision: 20230111
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC9822456
DOI: 10.3390/molecules28010305
PMID: 36615503
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules28010305