دورية أكاديمية
Feasibility of shape-sensing robotic-assisted bronchoscopy for biomarker identification in patients with thoracic malignancies.
| العنوان: | Feasibility of shape-sensing robotic-assisted bronchoscopy for biomarker identification in patients with thoracic malignancies. |
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| المؤلفون: | Connolly JG; Thoracic Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY., Kalchiem-Dekel O; Section of Interventional Pulmonology, Pulmonary Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY., Tan KS; Biostatistics Service, Department of Epidemiology and Biostatistics, Memorial Sloan Kettering Cancer Center, New York, NY., Dycoco J; Thoracic Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY., Chawla M; Section of Interventional Pulmonology, Pulmonary Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY., Rocco G; Thoracic Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY; Druckenmiller Center for Lung Cancer Research, Memorial Sloan Kettering Cancer Center, New York, NY., Park BJ; Thoracic Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY; Druckenmiller Center for Lung Cancer Research, Memorial Sloan Kettering Cancer Center, New York, NY., Lee RP; Section of Interventional Pulmonology, Pulmonary Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY., Beattie JA; Section of Interventional Pulmonology, Pulmonary Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY., Solomon SB; Interventional Radiology Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY., Ziv E; Interventional Radiology Service, Department of Radiology, Memorial Sloan Kettering Cancer Center, New York, NY., Adusumilli PS; Thoracic Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY; Druckenmiller Center for Lung Cancer Research, Memorial Sloan Kettering Cancer Center, New York, NY., Buonocore DJ; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY., Husta BC; Section of Interventional Pulmonology, Pulmonary Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY., Jones DR; Thoracic Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY; Druckenmiller Center for Lung Cancer Research, Memorial Sloan Kettering Cancer Center, New York, NY., Baine MK; Department of Pathology, Memorial Sloan Kettering Cancer Center, New York, NY., Bott MJ; Thoracic Service, Department of Surgery, Memorial Sloan Kettering Cancer Center, New York, NY; Druckenmiller Center for Lung Cancer Research, Memorial Sloan Kettering Cancer Center, New York, NY. Electronic address: bottm@mskcc.org. |
| المصدر: | The Journal of thoracic and cardiovascular surgery [J Thorac Cardiovasc Surg] 2023 Jul; Vol. 166 (1), pp. 231-240.e2. Date of Electronic Publication: 2022 Nov 25. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Mosby Country of Publication: United States NLM ID: 0376343 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-685X (Electronic) Linking ISSN: 00225223 NLM ISO Abbreviation: J Thorac Cardiovasc Surg Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: St. Louis, MO : Mosby Original Publication: St. Louis. |
| مواضيع طبية MeSH: | Carcinoma, Non-Small-Cell Lung*/genetics , Carcinoma, Non-Small-Cell Lung*/surgery , Carcinoma, Non-Small-Cell Lung*/chemistry , Lung Neoplasms*/genetics , Lung Neoplasms*/surgery , Lung Neoplasms*/chemistry , Robotic Surgical Procedures* , Thoracic Neoplasms*, Humans ; B7-H1 Antigen ; Bronchoscopy ; Retrospective Studies ; Feasibility Studies ; Biomarkers, Tumor/genetics ; Biomarkers, Tumor/analysis |
| مستخلص: | Objective: Molecular diagnostic assays require samples with high nucleic acid content to generate reliable data. Similarly, programmed death-ligand 1 (PD-L1) immunohistochemistry (IHC) requires samples with adequate tumor content. We investigated whether shape-sensing robotic-assisted bronchoscopy (ssRAB) provides adequate samples for molecular and predictive testing. Methods: We retrospectively identified diagnostic samples from a prospectively collected database. Pathologic reports were reviewed to assess adequacy of samples for molecular testing and feasibility of PD-L1 IHC. Tumor cellularity was quantified by an independent pathologist using paraffin-embedded sections. Univariable and multivariable linear regression models were constructed to assess associations between lesion- and procedure-related variables and tumor cellularity. Results: In total, 128 samples were analyzed: 104 primary lung cancers and 24 metastatic lesions. On initial pathologic assessment, ssRAB samples were deemed to be adequate for molecular testing in 84% of cases; on independent review of cellular blocks, median tumor cellularity was 60% (interquartile range, 25%-80%). Hybrid capture-based next-generation sequencing was successful for 25 of 26 samples (96%), polymerase chain reaction-based molecular testing (Idylla; Biocartis) was successful for 49 of 52 samples (94%), and PD-L1 IHC was successful for 61 of 67 samples (91%). Carcinoid and small cell carcinoma histologic subtype and adequacy on rapid on-site evaluation were associated with higher tumor cellularity. Conclusions: The ssRAB platform provided adequate tissue for next-generation sequencing, polymerase chain reaction-based molecular testing, and PD-L1 IHC in >80% of cases. Tumor histology and adequacy on intraoperative cytologic assessment might be associated with sample quality and suitability for downstream assays. (Copyright © 2022 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.) |
| معلومات مُعتمدة: | K08 CA245206 United States CA NCI NIH HHS; T32 CA009501 United States CA NCI NIH HHS; P30 CA008748 United States CA NCI NIH HHS; R01 CA240472 United States CA NCI NIH HHS; R01 CA217169 United States CA NCI NIH HHS |
| فهرسة مساهمة: | Keywords: molecular testing; next-generation sequencing; pulmonary nodule; robotic-assisted bronchoscopy; thoracic malignancy |
| المشرفين على المادة: | 0 (B7-H1 Antigen) 0 (Biomarkers, Tumor) |
| تواريخ الأحداث: | Date Created: 20230109 Date Completed: 20230616 Latest Revision: 20240702 |
| رمز التحديث: | 20240702 |
| مُعرف محوري في PubMed: | PMC10209350 |
| DOI: | 10.1016/j.jtcvs.2022.10.059 |
| PMID: | 36621452 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1097-685X |
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| DOI: | 10.1016/j.jtcvs.2022.10.059 |