دورية أكاديمية
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Improved Whole-Cell Biocatalyst for the Synthesis of Vitamin E Precursor 2,3,5-Trimethylhydroquinone.

التفاصيل البيبلوغرافية
العنوان: Improved Whole-Cell Biocatalyst for the Synthesis of Vitamin E Precursor 2,3,5-Trimethylhydroquinone.
المؤلفون: Ji J; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China.; Nanjing Key Laboratory of Quality and Safety of Agricultural Products, College of Food Science, Nanjing XiaoZhuang University, Nanjing 211171, Jiangsu, People's Republic of China., Zeng C; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China., Wu P; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China., Wang Y; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China., Chen X; Shanghai Fisheries Research Institute, Shanghai Fisheries Technical Extension Station, Shanghai 200433, People's Republic of China., Yan X; Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, Jiangsu, People's Republic of China.
المصدر: Journal of agricultural and food chemistry [J Agric Food Chem] 2023 Jan 18; Vol. 71 (2), pp. 1162-1169. Date of Electronic Publication: 2023 Jan 09.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: American Chemical Society Country of Publication: United States NLM ID: 0374755 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5118 (Electronic) Linking ISSN: 00218561 NLM ISO Abbreviation: J Agric Food Chem Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Washington, American Chemical Society.
مواضيع طبية MeSH: Diazonium Compounds* , Pyridines*, Vitamin E
مستخلص: 2,3,5-Trimethylhydroquinone (2,3,5-TMHQ) is the key precursor in the synthesis of vitamin E. It is still a major challenge to produce 2,3,5-TMHQ under mild reaction conditions by chemical methods. The monooxygenase system MpdAB can specifically catalyze the conversion of 2,3,6-trimethylphenol (2,3,6-TMP) to 2,3,5-TMHQ. However, the weak catalytic capacity of wild-type MpdA and the cytotoxicity of the substrate limited the production efficiency of 2,3,5-TMHQ. Here, homologous modeling and saturation mutation were performed to increase the catalytic activity of MpdA. Two variants, L128A and L128K, with higher activity toward 2,3,6-TMP (1.86-1.87-fold) were obtained. On the other hand, an evolved strain B5-4M-evolved with enhanced resistance to 2,3,6-TMP (8.15-fold higher for 1000 μM 2,3,6-TMP) was obtained through adaptive laboratory evolution. Subsequently, a 5.29-fold (or 4.87-fold) improvement in 2,3,5-TMHQ production was achieved by a strain B5-4M-evolved harboring L128K (or L128A) and MpdB, in comparison with that of the wild type (strain B5-4M expressing MpdAB). This study provides better genetic resources for producing 2,3,5-TMHQ and proves that the synthesis efficiency of 2,3,5-TMHQ can be improved through enzyme modification and adaptive laboratory evolution.
فهرسة مساهمة: Keywords: 2,3,5-trimethylhydroquinone; adaptive laboratory evolution; site-directed mutagenesis; whole-cell catalyst
المشرفين على المادة: 117141-31-8 (3-(2'-pyridyldithio)benzyldiazoacetate)
GSY6340N4A (2,3,5-trimethylquinol)
0 (Diazonium Compounds)
0 (Pyridines)
1406-18-4 (Vitamin E)
تواريخ الأحداث: Date Created: 20230109 Date Completed: 20230119 Latest Revision: 20230119
رمز التحديث: 20230120
DOI: 10.1021/acs.jafc.2c07768
PMID: 36621524
قاعدة البيانات: MEDLINE
الوصف
تدمد:1520-5118
DOI:10.1021/acs.jafc.2c07768