دورية أكاديمية
Molecular identification of Trypanosoma theileri in cattle from the Ecuadorian Amazon.
| العنوان: | Molecular identification of Trypanosoma theileri in cattle from the Ecuadorian Amazon. |
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| المؤلفون: | De la Cadena E; Research Group of Emerging and Neglected Diseases, Ecoepidemiology and Biodiversity, Health Science Faculty, Universidad Internacional SEK (UISEK), Quito 170120, Ecuador; Program of Master in Biomedicine, Health Sciences Faculty, Universidad Internacional SEK (UISEK), Quito 170120, Ecuador., Camacho M; Research Group of Emerging and Neglected Diseases, Ecoepidemiology and Biodiversity, Health Science Faculty, Universidad Internacional SEK (UISEK), Quito 170120, Ecuador; Program of Master in Biomedicine, Health Sciences Faculty, Universidad Internacional SEK (UISEK), Quito 170120, Ecuador., Vaca F; Unidad de Entomología Aplicada, Instituto de Investigaciones en Zoonosis (CIZ), Universidad Central del Ecuador, Quito, Ecuador., Enríquez S; Unidad de Entomología Aplicada, Instituto de Investigaciones en Zoonosis (CIZ), Universidad Central del Ecuador, Quito, Ecuador., Eleizalde MC; Centro de Estudios Biomédicos y Veterinarios (CEBIV), Instituto de Estudios Científicos y Tecnológicos (IDECYT), Universidad Nacional Experimental Simón Rodríguez (UNESR), Apartado Postal 47925, Caracas, Venezuela., Arrivillaga-Henríquez J; Unidad de Entomología Aplicada, Instituto de Investigaciones en Zoonosis (CIZ), Universidad Central del Ecuador, Quito, Ecuador; Línea de Salud Ambiental, Calidad y Biodiversidad, Área Ambiente y Territorio, THC-FACSO, Universidad Central del Ecuador, Quito, Ecuador., Mendoza M; Centro de Estudios Biomédicos y Veterinarios (CEBIV), Instituto de Estudios Científicos y Tecnológicos (IDECYT), Universidad Nacional Experimental Simón Rodríguez (UNESR), Apartado Postal 47925, Caracas, Venezuela., Navarro JC; Research Group of Emerging and Neglected Diseases, Ecoepidemiology and Biodiversity, Health Science Faculty, Universidad Internacional SEK (UISEK), Quito 170120, Ecuador; Program of Master in Biomedicine, Health Sciences Faculty, Universidad Internacional SEK (UISEK), Quito 170120, Ecuador., Ramírez-Iglesias JR; Research Group of Emerging and Neglected Diseases, Ecoepidemiology and Biodiversity, Health Science Faculty, Universidad Internacional SEK (UISEK), Quito 170120, Ecuador; Program of Master in Biomedicine, Health Sciences Faculty, Universidad Internacional SEK (UISEK), Quito 170120, Ecuador. Electronic address: jose.ramirez@uisek.edu.ec. |
| المصدر: | Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2023 Jan; Vol. 37, pp. 100824. Date of Electronic Publication: 2022 Dec 17. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101680410 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2405-9390 (Electronic) Linking ISSN: 24059390 NLM ISO Abbreviation: Vet Parasitol Reg Stud Reports Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam : Elsevier, [2015]- |
| مواضيع طبية MeSH: | Cattle Diseases*/parasitology , Trypanosoma*/genetics , Trypanosomiasis*/epidemiology , Trypanosomiasis*/veterinary , Trypanosomiasis*/parasitology, Cattle ; Animals ; Ecuador/epidemiology ; Ruminants |
| مستخلص: | Trypanosoma theileri is a cosmopolitan opportunistic haemoparasite described in wild and domestic ruminants, and also in arthropod vectors. The presence of this parasite has been reported in several South American countries, including Amazonian regions. Despite the importance of livestock production, Ecuador possesses scarce studies about trypanosomosis and no T. theileri reports in its territory. Here, we showed molecular evidences of the presence of T. theileri in cattle from a province located in the Ecuadorian Amazon. Bovine blood samples were collected from 2014 to 2019, during campaigns to detect haemoparasites in the Ecuadorian provinces of Orellana and Sucumbíos. DNA was extracted from the buffy coat and used in PCR assays with three different molecular markers, ITS1, 18S and Cathepsin L-like. T. theileri was detected only in the Sucumbíos province, with a specific molecular prevalence of 8.6% (3/35) using the three primers and an additional animal detected as positive (11.4% prevalence) only by the ITS1 marker. DNA sequences derived from the generated amplicons were subjected to phylogenetics maximum parsimony and maximum likelihood analysis, which indicate the presence of TthI and TthII genotypes circulating in the evaluated animals. Molecular surveillance should be continually implemented in Ecuador in order to deepen the epidemiological and evolutionary knowledge about T. theileri as well other haemoparasites in the amazon parts of the country. Competing Interests: Declaration of Competing Interest The authors declare that they have no competing interests. (Copyright © 2022 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cattle; Ecuadorian Amazon; Molecular surveillance; Phylogeny; Trypanosoma theileri |
| تواريخ الأحداث: | Date Created: 20230109 Date Completed: 20230111 Latest Revision: 20230222 |
| رمز التحديث: | 20230223 |
| DOI: | 10.1016/j.vprsr.2022.100824 |
| PMID: | 36623904 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2405-9390 |
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| DOI: | 10.1016/j.vprsr.2022.100824 |