دورية أكاديمية
Improvement of sperm quality of the depik fish, rasbora tawarensis, after cryopreservation using antioxidant.
| العنوان: | Improvement of sperm quality of the depik fish, rasbora tawarensis, after cryopreservation using antioxidant. |
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| المؤلفون: | Muchlisin ZA; Department of Aquaculture, Faculty of Marine and Fisheries Sciences; Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia. muchlisinza@unsyiah.ac.id., Afriani D; Faculty of Mathematics and Natural Sciences, Unversitas Syiah Kuala, Banda Aceh 23111, Indonesia., Eriani K; Faculty of Mathematics and Natural Sciences, Unversitas Syiah Kuala, Banda Aceh 23111, Indonesia., Hasri I; Veterinary and Fisheries Affair of Aceh Tengah District, Takengon, Indonesia., Nur FM; Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia., Maulida S; Department of Aquaculture, Faculty of Marine and Fisheries Sciences; Graduate School of Mathematics and Applied Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia., Handayani LS; Department of Aquaculture, Faculty of Marine and Fisheries Sciences, Universitas Syiah Kuala, Banda Aceh 23111, Indonesia., Kocabas FK; Tunceli University, Fisheries Faculty, 62000 Tunceli, Turkey., Siti-Azizah MS; Institute of Marine Biotechnology, University Malaysia Terengganu, Kuala Terengganu, Malaysia. |
| المصدر: | Cryo letters [Cryo Letters] 2023 Jan-Feb; Vol. 44 (1), pp. 13-19. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: CryoLetters Country of Publication: England NLM ID: 9891832 Publication Model: Print Cited Medium: Print ISSN: 0143-2044 (Print) Linking ISSN: 01432044 NLM ISO Abbreviation: Cryo Letters Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London, Eng. : CryoLetters Original Publication: [London : Royal Veterinary College, |
| مواضيع طبية MeSH: | Semen Preservation*/veterinary , Cypriniformes*, Animals ; Male ; Antioxidants/pharmacology ; Ringer's Solution ; Cryopreservation/veterinary ; Semen ; Sperm Motility ; Spermatozoa ; Glutathione/pharmacology ; Cryoprotective Agents/pharmacology |
| مستخلص: | Background: The cryopreservation of the sperm of the depik fish, Rasbora tawarensis, has previously been developed. However, the quality of the sperm post cryopreservation was not satisfactory and might be improved through the application of antioxidants. Objective: To determine the most suitable antioxidant for the cryopreservation of the depik fish spermatozoa. Materials and Methods: A completely randomized design with a non-factorial experiment was used and the tested antioxidants were glutathione, beta-carotene, ascorbic acid, and butylated hydroxytoluene (BHT) at 6 % concentrations. All treatments had three replications. The sperms were collected from 10 male fishes and diluted with Ringer solution in a ratio of 1: 20 (v/v, sperm: Ringer solution). Then 5% DMSO and 5 % egg yolk were added to the diluted sperms. Furthermore, 6 % of the tested antioxidants were added to the diluents, and then, cryopreservation was carried out in liquid nitrogen for 14 days. Results: The ANOVA test showed that the application of antioxidants significantly affected the sperm motility, fertility, and hatching rates of the eggs (P < 0.05). Furthermore, the antioxidants also protected the sperm cells during cryopreservation, with glutathione being the best antioxidant. Conclusion: The application of antioxidants during the cryopreservation of depik fish sperm had a significant effect on motility, fertility and hatchability of eggs post-cryo. Furthermore, glutathione was the most suitable antioxidant. doi.org/10.54680/fr23110110312. |
| المشرفين على المادة: | 0 (Antioxidants) 8026-10-6 (Ringer's Solution) GAN16C9B8O (Glutathione) 0 (Cryoprotective Agents) |
| تواريخ الأحداث: | Date Created: 20230111 Date Completed: 20230113 Latest Revision: 20230516 |
| رمز التحديث: | 20231215 |
| PMID: | 36629837 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0143-2044 |
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