دورية أكاديمية
أعرض في EDS

TEAD Proteins Associate With DNA Repair Proteins to Facilitate Cellular Recovery From DNA Damage.

التفاصيل البيبلوغرافية
العنوان: TEAD Proteins Associate With DNA Repair Proteins to Facilitate Cellular Recovery From DNA Damage.
المؤلفون: Calses PC; Departments of Discovery Oncology, Genentech Inc, South San Francisco, California, USA; Department of Microchemistry, Proteomics & Lipidomics, Genentech Inc, South San Francisco, California, USA., Pham VC; Department of Microchemistry, Proteomics & Lipidomics, Genentech Inc, South San Francisco, California, USA., Guarnaccia AD; Departments of Discovery Oncology, Genentech Inc, South San Francisco, California, USA; Department of Microchemistry, Proteomics & Lipidomics, Genentech Inc, South San Francisco, California, USA., Choi M; Department of Microchemistry, Proteomics & Lipidomics, Genentech Inc, South San Francisco, California, USA., Verschueren E; Department of Microchemistry, Proteomics & Lipidomics, Genentech Inc, South San Francisco, California, USA., Bakker ST; UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, California, USA., Pham TH; Departments of Discovery Oncology, Genentech Inc, South San Francisco, California, USA., Hinkle T; Department of Microchemistry, Proteomics & Lipidomics, Genentech Inc, South San Francisco, California, USA., Liu C; Departments of Discovery Oncology, Genentech Inc, South San Francisco, California, USA., Chang MT; Department of Bioinformatics, Genentech Inc, South San Francisco, California, USA., Kljavin N; Department of Molecular Oncology, Genentech Inc, South San Francisco, California, USA., Bakalarski C; Department of Microchemistry, Proteomics & Lipidomics, Genentech Inc, South San Francisco, California, USA., Haley B; Departments of Discovery Oncology, Genentech Inc, South San Francisco, California, USA., Zou J; Department of Biology, Research Service Division, WuXi AppTec, Shanghai, China., Yan C; Department of Biology, Research Service Division, WuXi AppTec, Shanghai, China., Song X; Department of Biology, Research Service Division, WuXi AppTec, Shanghai, China., Lin X; Department of Biology, Research Service Division, WuXi AppTec, Shanghai, China., Rowntree R; Department of Molecular Oncology, Genentech Inc, South San Francisco, California, USA., Ashworth A; UCSF Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, California, USA., Dey A; Departments of Discovery Oncology, Genentech Inc, South San Francisco, California, USA. Electronic address: anweshad@gene.com., Lill JR; Department of Microchemistry, Proteomics & Lipidomics, Genentech Inc, South San Francisco, California, USA. Electronic address: jlill@gene.com.
المصدر: Molecular & cellular proteomics : MCP [Mol Cell Proteomics] 2023 Feb; Vol. 22 (2), pp. 100496. Date of Electronic Publication: 2023 Jan 12.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology Country of Publication: United States NLM ID: 101125647 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1535-9484 (Electronic) Linking ISSN: 15359476 NLM ISO Abbreviation: Mol Cell Proteomics Subsets: MEDLINE
أسماء مطبوعة: Publication: 2021- : [New York, NY] : Elsevier Inc. on behalf of American Society for Biochemistry and Molecular Biology
Original Publication: Bethesda, MD : American Society for Biochemistry and Molecular Biology, [2002-
مواضيع طبية MeSH: DNA Damage* , DNA Repair*/physiology , TEA Domain Transcription Factors*/metabolism, Humans ; Carcinogenesis/metabolism ; DNA-Binding Proteins/metabolism ; Transcription Factors/metabolism
مستخلص: Transcriptional enhanced associate domain family members 1 to 4 (TEADs) are a family of four transcription factors and the major transcriptional effectors of the Hippo pathway. In order to activate transcription, TEADs rely on interactions with other proteins, such as the transcriptional effectors Yes-associated protein and transcriptional co-activator with PDZ-binding motif. Nuclear protein interactions involving TEADs influence the transcriptional regulation of genes involved in cell growth, tissue homeostasis, and tumorigenesis. Clearly, protein interactions for TEADs are functionally important, but the full repertoire of TEAD interaction partners remains unknown. Here, we employed an affinity purification mass spectrometry approach to identify nuclear interacting partners of TEADs. We performed affinity purification mass spectrometry experiment in parallel in two different cell types and compared a wildtype TEAD bait protein to a nuclear localization sequence mutant that does not localize to the nucleus. We quantified the results using SAINT analysis and found a significant enrichment of proteins linked to DNA damage including X-ray repair cross-complementing protein 5 (XRCC5), X-ray repair cross-complementing protein 6 (XRCC6), poly(ADP-ribose) polymerase 1 (PARP1), and Rap1-interacting factor 1 (RIF1). In cellular assays, we found that TEADs co-localize with DNA damage-induced nuclear foci marked by histone H2AX phosphorylated on S139 (γH2AX) and Rap1-interacting factor 1. We also found that depletion of TEAD proteins makes cells more susceptible to DNA damage by various agents and that depletion of TEADs promotes genomic instability. Additionally, depleting TEADs dampens the efficiency of DNA double-stranded break repair in reporter assays. Our results connect TEADs to DNA damage response processes, positioning DNA damage as an important avenue for further research of TEAD proteins.
Competing Interests: Conflict of interest A. A. is co-founder of Tango Therapeutics, Azkarra Therapeutics, Ovibio Corporation; a consultant for SPARC, Bluestar, TopoRx, ProLynx, Earli, Cura, GSK; a member of the SAB of Genentech and GLAdiator; receives grant/research support from SPARC and AstraZeneca; holds patents on the use of PARP inhibitors held jointly with AstraZeneca which he has benefited financially (and may do so in the future). All Genentech authors are employees and shareholders at Roche and have no conflict of interest.
(Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
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معلومات مُعتمدة: P30 CA082103 United States CA NCI NIH HHS
فهرسة مساهمة: Keywords: AP-MS; DNA damage; Hippo pathway; TEAD; transcription
المشرفين على المادة: 0 (DNA-Binding Proteins)
0 (Transcription Factors)
0 (TEAD1 protein, human)
0 (TEA Domain Transcription Factors)
تواريخ الأحداث: Date Created: 20230114 Date Completed: 20230316 Latest Revision: 20240426
رمز التحديث: 20240426
مُعرف محوري في PubMed: PMC9947421
DOI: 10.1016/j.mcpro.2023.100496
PMID: 36640924
قاعدة البيانات: MEDLINE
الوصف
تدمد:1535-9484
DOI:10.1016/j.mcpro.2023.100496