دورية أكاديمية
Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR.
| العنوان: | Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR. |
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| المؤلفون: | Sales ML; Departamento de Medicina Veterinária Preventiva, Escola de Veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Fonseca Júnior AA; Laboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Brazil. antonio.fonseca@agro.gov.br.; UNIFEMM - Centro Universitário, Sete Lagoas, Brazil. antonio.fonseca@agro.gov.br., Pinheiro de Oliveira TF; Laboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Brazil., de Araújo Lopes A; Laboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Brazil., Rivetti Júnior AV; Laboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Brazil., Camargos MF; Laboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Brazil., Dos Reis JKP; Departamento de Medicina Veterinária Preventiva, Escola de Veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, Brazil. |
| المصدر: | Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2023 Mar; Vol. 54 (1), pp. 491-497. Date of Electronic Publication: 2023 Jan 16. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Brazil NLM ID: 101095924 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1678-4405 (Electronic) Linking ISSN: 15178382 NLM ISO Abbreviation: Braz J Microbiol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Switzerland, AG : Springer International Publishing Original Publication: Rio de Janeiro, RJ, Brasil : Sociedade Brasileira de Microbiologia |
| مواضيع طبية MeSH: | Vesiculovirus*/genetics , Vesicular Stomatitis*, Animals ; Reverse Transcriptase Polymerase Chain Reaction ; Phylogeny ; RNA, Viral/genetics |
| مستخلص: | Vesicular stomatitis caused by Alagoas vesiculovirus (VSAV) has generated disease outbreaks in Brazil, mainly in the northeast region. Phylogenetic studies divide the isolates into three distinct genotypes (A, B, and C). However, there is no description of how this genetic divergence reflects on the phenotype of VSAV isolates such as in vitro replication fitness. Therefore, the objective of this work was to evaluate the ability of three distinct genotypes of Brazilian isolates of VSAV to grow in different cell-culture lines (BHK-21, Vero, and NCI-H1299). Quantification of viral RNA was performed using RT-PCR digital droplet from supernatant of cell culture collected every 4 h for a period of 24 h of viral growth in three different cell lines (BHK-21, Vero, and NCI-H1299). It was observed that the genotype C isolate has the lowest replication efficiency among the three analyzed viruses, without major changes in the copies of viral RNA over the entire time of the study. (© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.) |
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| فهرسة مساهمة: | Keywords: Alagoas vesiculovirus; Cell culture; Vesicular diseases; Viral growth |
| المشرفين على المادة: | 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20230116 Date Completed: 20230224 Latest Revision: 20230224 |
| رمز التحديث: | 20230224 |
| مُعرف محوري في PubMed: | PMC9841932 |
| DOI: | 10.1007/s42770-023-00902-w |
| PMID: | 36645640 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1678-4405 |
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| DOI: | 10.1007/s42770-023-00902-w |