دورية أكاديمية
Wolbachia Promotes Its Own Uptake by Host Cells.
| العنوان: | Wolbachia Promotes Its Own Uptake by Host Cells. |
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| المؤلفون: | Nevalainen LB; Department of Biology, Indiana University, Bloomington, Indiana, USA., Layton EM; Department of Biology, Indiana University, Bloomington, Indiana, USA., Newton ILG; Department of Biology, Indiana University, Bloomington, Indiana, USA. |
| المصدر: | Infection and immunity [Infect Immun] 2023 Feb 16; Vol. 91 (2), pp. e0055722. Date of Electronic Publication: 2023 Jan 17. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0246127 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1098-5522 (Electronic) Linking ISSN: 00199567 NLM ISO Abbreviation: Infect Immun Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington, DC : American Society For Microbiology Original Publication: [Bethesda, Md.] American Society for Microbiology. |
| مواضيع طبية MeSH: | Wolbachia*, Animals ; Vacuoles ; Actins ; Symbiosis ; Drosophila melanogaster/microbiology |
| مستخلص: | Wolbachia pipientis is an incredibly widespread bacterial symbiont of insects, present in an estimated 25 to 52% of species worldwide. Wolbachia is faithfully maternally transmitted both in a laboratory setting and in the wild. In an established infection, Wolbachia is primarily intracellular, residing within host-derived vacuoles that are associated with the endoplasmic reticulum. However, Wolbachia also frequently transfers between host species, requiring an extracellular stage to its life cycle. Indeed, Wolbachia has been moved between insect species for the precise goal of controlling populations. The use of Wolbachia in this application requires that we better understand how it initiates and establishes new infections. Here, we designed a novel method for live tracking Wolbachia cells during infection using a combination of stains and microscopy. We show that live Wolbachia cells are taken up by host cells at a much faster rate than dead Wolbachia cells, indicating that Wolbachia bacteria play a role in their own uptake and that Wolbachia colonization is not just a passive process. We also show that the host actin cytoskeleton must be intact for this to occur and that drugs that disrupt the actin cytoskeleton effectively abrogate Wolbachia uptake. The development of this live infection assay will assist in future efforts to characterize Wolbachia factors used during host infection. |
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| معلومات مُعتمدة: | R01 AI144430 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: Drosophila; Wolbachia; cytochalasin D; infection |
| المشرفين على المادة: | 0 (Actins) |
| تواريخ الأحداث: | Date Created: 20230117 Date Completed: 20230222 Latest Revision: 20230718 |
| رمز التحديث: | 20230718 |
| مُعرف محوري في PubMed: | PMC9933726 |
| DOI: | 10.1128/iai.00557-22 |
| PMID: | 36648231 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1098-5522 |
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| DOI: | 10.1128/iai.00557-22 |